12 research outputs found

    Dendrogram displaying the PFGE profiles of the 43 isolates.

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    <p>The strain number, origin, source, sequence type (ST), and H<sub>2</sub>S phenotype are shown for each strain. +, H<sub>2</sub>S-producing isolate; −, non-H<sub>2</sub>S-producing isolate.</p

    Sequence alignment of the <i>phs</i> gene and the protein.

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    <p>A nonsense mutation at position 208 of the <i>phsA</i> gene results in the replacement of a sense codon (CAG) with a termination codon (UAG) leading to the premature termination of <i>phsA</i>. The first sequence, <i>phsA</i>, is based on <i>S</i>. enterica serotype Typhimurium strain LT2 (GenBank AE006468). *, termination codon; +, H<sub>2</sub>S-producing isolate; −, non-H<sub>2</sub>S-producing isolate.</p

    Additional file 1: of Antibiotic resistance and molecular characterization of the hydrogen sulfide-negative phenotype among diverse Salmonella serovars in China

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    Table S1. Reference strains for phs operon sequence analysis. Table S2. GenBank accession numbers for phs operon sequences from 46 H2S-negative Salmonella isolates. Table S3. Mutations detected in the phsB and phsC genes of H2S-negative Salmonella isolates. (DOC 71 kb

    An outbreak of acute respiratory disease caused by a virus associated RNA II gene mutation strain of human adenovirus 7 in China, 2015

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    <div><p>Human adenovirus 7 (HAdV-7) strains are a major cause of acute respiratory disease (ARD) among adults and children, associated with fatal pneumonia. An ARD outbreak caused by HAdV-7 that involved 739 college students was reported in this article. To better understand the underlying cause of this large-scale epidemic, virus strains were isolated from infected patients and sequence variations of the whole genome sequence were detected. Evolutionary trees and alignment results indicated that the major capsid protein genes hexon and fibre were strongly conserved among serotype 7 strains in China at that time. Instead, the HAdV-7 strains presented three thymine deletions in the virus associated RNA (VA RNA) II terminal region. We also found that the mutation might lead to increased mRNA expression of an adjacent gene, L1 52/55K, and thus promoted faster growth. These findings suggest that sequence variation of VA RNA II gene was a potential cause of such a severe HAdV-7 infection and this gene should be a new-emerging factor to be monitored for better understanding of HAdV-7 infection.</p></div

    Growth kinetics of Wuhan and CDC228 strains.

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    <p>Virus titres were determined in human A549 cells cultured in 12-well plates by detecting virus genomes using real-time PCR (n = 3, mean ± SEM, **<i>P</i> ≤ 0.01, ***<i>P</i> ≤ 0.001 <i>vs</i>. CDC228).</p

    L1 52/55K mRNA expression assay using real-time PCR.

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    <p>L1 52/55K mRNA expression against (a) VA RNA, n = 3, mean ± SEM, **<i>P</i> = 0.0039 <i>vs</i>. CDC228; and (b) E1, n = 3, mean ± SEM, **<i>P</i> = 0.0098 <i>vs</i>. CDC228. CDC228, XY1, and WH correspond to KJ019884, KJ019880, and Wuhan strains, respectively.</p