16 research outputs found

    Utilization of Different Concentration Sugarcane Molasses to the Quality of Goat Feces-Chicken Excreta-Coconut Husk Organic Liquid Fertilizer

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    Indonesia is an agricultural country that certainly has the potential for agricultural waste and by-products. Some of those in Indonesia are livestock manure, coconut husk, and sugarcane molasses. Utilization of this waste is limited, which is causing various environmental problems such as environmental pollution. The study is aimed to determine the quality of organic liquid fertilizer made by raw material from goat feces-chicken excreta-coconut husk with various sugarcane molasses concentrations (P0= 0%; P1= 5%; P3= 15%; P4= 20%; P5= 25%). The fermentation was carried out anaerobically for 21 days. The parameters of temperature, pH, organoleptic (color and odor), levels of C-organic, nitrogen (N) total, phosphorus (P) total, potassium (K) total, and biological tests were observed. Biological tests were conducted in the growth of mung bean (Vigna radiata) and spinach (Amaranthus tricolor). Data were analyzed using one-way ANOVA. Variations in the addition of molasses sugarcane did not affect the liquid fertilizer's temperature and pH during fermentation. However, the treatments had positive effect on organoleptic test, C-organic, N total, P total, K total, and biological tests. The addition of 15% sugarcane molasses (P3) showed a dominating performance toward most of the parameters tested. The P3 treatment produced liquid fertilizer with a pleasant smell, and the contents of C-organic, total N, total P, and total K were 2.12%, 0.25%, 0.13%, and 0.13%,  respectively. and produce spinach as a biological test with plant height, number of leaves, leaf width, stem diameter, and root length were 21.82 cm, 6.66 sheets, 3.59 cm, 4.09 mm, and 14.67 cm, respectively

    Pembuatan Asap Cair Guna Mendukung Pertanian Organik di Desa Bulusari, Kecamatan Kalipuro, Kabupaten Banyuwangi

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    Bulusari merupakan desa yang terletak di Kecamatan Kalipuro. Wilayah desa ini terdiri atas permukiman, lahan pertanian, dan perkebunan. Masyarakat Desa Bulusari bermukim di tiga dusun, yaitu Bulupayung, Kopensere, dan Plampang. Permukiman warga memiliki tipikal berupa rumah khas perkebunan. Kopi dan sedikit cengkih banyak ditanam di sekeliling rumah warga. Lahan pertanian ditanami padi, ketela pohon, kelapa, dan buah-buahan. Di Desa Bulusari juga terdapat usaha serkel kayu. Usaha serkel kayu tersebut menyisakan banyak limbah potongan kayu. Tujuan kegiatan ini adalah membantu masyarakat untuk memanfaatkan limbah kayu dan menghasilkan produk unggulan. Metode pelaksanaan kegiatan ini adalah introduksi inovasi pemanfaatan limbah kayu menjadi asap cair; mendesain dan membuat alat; praktik penggunaan alat; dan pengaplikasian penggunaan asap cair. Teknologi yang diterapkan mampu menghasilkan asap cair dengan grade 3 dan grade 2. Kegunaan asap cair ini adalah sebagai pestisida nabati/organik dan pengawet makanan. Pembuatan asap cair diharapkan dapat meningkatkan ketersediaan pestisida organik dan menciptakan lapangan pekerjaan baru bagi masyarakat Desa Bulusar

    The Effect of Young Coconut Husk on the Quality of Goat Manure-Chicken Excreta Bioculture

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    The negative impact of chemical fertilizers use is soil fertility declining. The situation occurs because of biological, physical, and chemical properties of the soil is decreased. Agricultural waste is a large commodity which utilization can still be optimized, for example by using as raw material for organic liquid fertilizer. Agricultural wastes that have good quality as fertilizer include goat manure, chicken excreta, and coconut husk. The utilization of agricultural waste as organic fertilizer is one way of creating sustainable agriculture. This study aims to test the quality of liquid fertilizer (bioculture) of goat manure and chicken excreta by adding various levels of coconut husk. Bioculture is made by anaerobic fermentation for 21 days. The parameters observed included levels of C-organic, N, P, and K bioculture, as well as germination tests to determine the presence of phytotoxins. The data were analyzed using one way ANOVA. The treatment of P5 (5% coconut husk) showed the highest levels of N and P, while the K content was not significantly different from the treatment of P4 (2.5% coconut husk). The germination test showed no phytotoxin activity in bioculture

    SRY Gene Marker Differences in Native and Crossbreed Cattle

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    This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples

    A Maluku Sea intermediate western boundary current connecting Pacific Ocean circulation to the Indonesian Throughflow

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    © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Yuan, D., Yin, X., Li, X., Corvianawatie, C., Wang, Z., Li, Y., Yang, Y., Hu, X., Wang, J., Tan, S., Surinati, D., Purwandana, A., Wardana, A., Ismail, M., Budiman, A., Bayhaqi, A., Avianto, P., Santoso, P., Kusmanto, E., Dirhamsyah, Arifin, Z., & Pratt, L. A Maluku Sea intermediate western boundary current connecting Pacific Ocean circulation to the Indonesian Throughflow. Nature Communications, 13(1), (2022): 2093, https://doi.org/10.1038/s41467-022-29617-6.The Indonesian Throughflow plays an important role in the global ocean circulation and climate. Existing studies of the Indonesian Throughflow have focused on the Makassar Strait and the exit straits, where the upper thermocline currents carry North Pacific waters to the Indian Ocean. Here we show, using mooring observations, that a previous unknown intermediate western boundary current (with the core at ~1000 m depth) exists in the Maluku Sea, which transports intermediate waters (primarily the Antarctic Intermediate Water) from the Pacific into the Seram-Banda Seas through the Lifamatola Passage above the bottom overflow. Our results suggest the importance of the western boundary current in global ocean intermediate circulation and overturn. We anticipate that our study is the beginning of more extensive investigations of the intermediate circulation of the Indo-Pacific ocean in global overturn, which shall improve our understanding of ocean heat and CO2 storages significantly.This study is supported by NSFC (D.Y., Z.W., Y.L., Y.Y., S.T., J.W., and X.L.: 41720104008; D.Y., J.W., Y.L., X.L., Y.Y., S.T., X.H., and X.Y.: 91858204), the National Key Research and Development Program of China (D.Y. and X.L.: 2020YFA0608800), CAS (D.Y., Z.W., J.W., and Y.L.: XDB42000000), projects. Affiliations 1 and 2 share the first position. D.Y. is supported by QMSNL (2018SDKJ0104-02), and Shandong Provincial (U1606402) and the “Kunpeng Outstanding Scholar Program” of the FIO/NMR of China, J.W. supported by NSFC (41776011), Z.W. by NSFC (41876025)

    Pemberdayaan Karang Taruna Melalui Budidaya Lele Dengan Teknologi Bioflok Di Desa Kedungringin Kecamatan Muncar Kabupaten Banyuwangi

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    Desa Kedungringin merupakan salah satu desa di kecamatan Muncar Banyuwangi. Sebagian besar penduduk Desa Kedungringin bermata pencarian sebagai petani, pembudidaya ikan, dan peternak. Sebagaian masyarakat Desa Kedungringin memilih untuk budidaya ikan lele Karena tingginya permintaan konsumen djpasaran selain itu mudah dalam pemeliharaan, dan masa panen yang tergolong cepat sekitar ± 3 bulan. Masyarakat Desa Kedungringin masih menerapkan sistem budidaya lele secara konvensional sehingga memiliki dampak kurang menguntungkan bagi petani ikan. Tujuan dari program pengabdian ini untuk meningkatkan produktivitas budidaya ikan lele sehingga mitra dapat mencukupi permintaan pasar, salah satunya dengan teknik bioflok. Teknik bioflok memiliki banyak kelebihan yaitu dapat mempersingkat waktu panen dan penggunaan pakan yang lebih e/isien sehingga hasil panen meningkat dan mitra bisa mendapatkan keuntungan yang optimal. Program pengabdian kepada masyrakat ini terbagi menjadi 3 metode yaitu penyuluhan, pelatihan, dan pendampingan. Berdasarkan hasil pengabdian mitra menunjukkan tingkat partispasi dan antusiasme yang tinggi dalam menjalankan program ini dan harapannya dengan berjalan program ini secara lancar dapat meningkatkan kesejahteraan mitra dan masyrakat sekitar di Desa Kedungringin. Kata kunci : Budidaya lele, bioflok, pelatiha

    Phylogenic Tree Analysis of Angus Grade Cattle

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    Studies about Indonesian cattle genetic purity should be encourage to determine the value of blood relationship and ancestors prediction, so the effort to improve livestock quality could be done correctly. This can be done by studies based on Cytochrome b gene that is only inherited maternally. The samples are 15 Angus Grade cattles from Sragen, Central Java, which have been reared since 1980s. The comparative datas were 54 Cytochrome b gene reference sequences from various breeds and countries (GenBank). The phylogenetic tree arranged by Mega 6 software with maximum likelihood method (Bootstrap values 1000, Tamura-Nei parameter) used 8 out-group Cytochrome b genes sequence comparators (GenBank). The results obtained that the Angus grade cattle had 2 branches ancestors, that branch belongs to Banteng (Bos javanicus) and branch belong to Domestic Cattle (Bos indicus; Bos taurus), with majority of the sample (n=13) were at the Banteng branch. The result indicates that most of Indonesian cattle presently, both crosses and local, had Banteng maternal ancestor and a small portion had close ancestor with Bos indicus or Bos taurus. Strong presumption that ancestors of native cattle in Indonesia and the surrounding regions are species of Bos javanicus, Bos sauveli, Bos grunniens and Bos frontalis

    Analisis Filogenetik pada Sapi Peranakan Angus

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    Studi terkait kemurnian ternak sapi di Indonesia perlu dilakukan guna mengetahui nilai kekerabatan dan memprediksi nenek moyang, agar usaha pemuliaan ternak dapat dilakukan secara tepat. Hal tersebut dapat dilakukan dengan menggunakan studi kekerabatan berdasar gen Cytochrome b yang hanya diwariskan secara maternal. Penelitian ini menggunakan sampel sebanyak 15 ekor sapi Peranakan Angus, berasal dari Sragen, Jawa Tengah, yang telah dibudidayakan sejak tahun 1980-an oleh peternak rakyat. Materi pembanding berupa 54 data sekuen referensi gen Cytochrome b dari berbagai sapi dan negara (GenBank). Pembuatan pohon filogenetik menggunakan program Mega 6 dengan metode maximum likelihood (1000 nilai Bootstrap, parameter Tamura-Nei) dengan pembanding 8 sekuen gen Cytochrome b individu out-group (GenBank). Hasil yang didapatkan ialah sapi Peranakan Angus yang disampel terbagi dalam 2 cabang moyang, yaitu cabang yang memuat Banteng (Bos javanicus) dan cabang Sapi Domestik (Bos indicus dan Bos taurus), dengan mayoritas sampel (n= 13) berada pada cabang Banteng. Hal ini menunjukkan bahwa sebagian besar sapi Indonesia masa kini, baik persilangan maupun lokal memiliki moyang maternal yang berasal dari Banteng dan sebagian kecil memiliki kedekatan moyang dengan Bos indicus atau Bos taurus. Kuat praduga bahwa moyang sapi asli Indonesia dan sekitarnya ialah dari spesies Bos javanicus, Bos sauveli, Bos grunniens, dan Bos frontalis

    Restriction Enzyme Mapping of MC4R Gene in Bligon Goat Using Bioedit Program

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    Restriction enzymes are a fundamental tool of molecular biology for determination of diversity and in vivo function. Many research used restriction enzyme for genotyping mammals based on MC4R gene. The objective this study to map the restriction enzyme of MC4R gene in Bligon goat. The mapping of restriction enzyme on MC4R gene was carried in Bligon goat (n=10). MC4R gene was amplified using specific primer forward and reverse, resulting 642 bp of amplification products. We discovered 5 enzymes (FatI, NlaIII, RsaI, Acc651 and KpnI) which recognized 2 SNPs region based on restriction mapping using Bioedit program. There were three recommended enzymes (RsaI, Acc651 and KpnI) for genotyping the Bligon goat. In the present study, an individual with CC genotype produced 2 fragments with enzyme RsaI and 1 fragment with Acc651 and KpnI. An individual with CT genotype produced 4 fragments with enzyme RsaI and 3 fragments with Acc651 and KpnI. In conclusion, we suggested that restriction enzymes RsaI, Acc651 and Kpn1 may be used for genotyping of a targeted gene using PCR-RFLP method for Bligon goat in the future research
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