156 research outputs found

    Protein networks – A driving force for discovery in plant science

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    A Model for the Biosynthesis and Transport of Plasma Membrane-Associated Signaling Receptors to the Cell Surface

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    Intracellular protein transport is emerging as critical in determining the outcome of receptor-activated signal transduction pathways. In plants, relatively little is known about the nature of the molecular components and mechanisms involved in coordinating receptor synthesis and transport to the cell surface. Recent advances in this field indicate that signaling pathways and intracellular transport machinery converge and coordinate to render receptors competent for signaling at their plasma membrane (PM) activity sites. The biogenesis and transport to the cell surface of signaling receptors appears to require both general trafficking and receptor-specific factors. Several molecular determinants, residing or associated with compartments of the secretory pathway and known to influence aspects in receptor biogenesis, are discussed and integrated into a predictive cooperative model for the functional expression of signaling receptors at the PM

    Arabidopsis RTNLB1 and RTNLB2 reticulon-like proteins regulate intracellular trafficking and activity of the FLS2 immune receptor

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    Receptors localized at the plasma membrane are critical for the recognition of pathogens. The molecular determinants that regulate receptor transport to the plasma membrane are poorly understood. In a screen for proteins that interact with the FLAGELIN-SENSITIVE2 (FLS2) receptor using Arabidopsis thaliana protein microarrays, we identified the reticulon-like protein RTNLB1. We showed that FLS2 interacts in vivo with both RTNLB1 and its homolog RTNLB2 and that a Ser-rich region in the N-terminal tail of RTNLB1 is critical for the interaction with FLS2. Transgenic plants that lack RTNLB1 and RTNLB2 (rtnlb1 rtnlb2) or overexpress RTNLB1 (RTNLB1ox) exhibit reduced activation of FLS2-dependent signaling and increased susceptibility to pathogens. In both rtnlb1 rtnlb2 and RTNLB1ox, FLS2 accumulation at the plasma membrane was significantly affected compared with the wild type. Transient overexpression of RTNLB1 led to FLS2 retention in the endoplasmic reticulum (ER) and affected FLS2 glycosylation but not FLS2 stability. Removal of the critical N-terminal Serrich region or either of the two Tyr-dependent sorting motifs from RTNLB1 causes partial reversion of the negative effects of excess RTNLB1 on FLS2 transport out of the ER and accumulation at the membrane. The results are consistent with a model whereby RTNLB1 and RTNLB2 regulate the transport of newly synthesized FLS2 to the plasma membrane. © 2011 American Society of Plant Biologists. All rights reserved

    UNIVARIATE AND MULTIVARIATE ANALYSIS OF SOME GARLIC LANDRACES FOR BULBS TRAITS

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    The objectives of this paper were to evaluate 12 garlic landraces collected fromSouth-Western saline area of Timis County regarding the bulbs traits and interrelationship among them, with a view to exploiting some of these landraces directly in the crop or using these landraces in breeding program. In this region growers use mostly garlic landraces with a high adaptability to specific environmental conditions, like soil salinity.The results prove the existence of a large phenotypic diversity of bulb traits, even between landraces from the same location (Livezile, Sanmartin, Foieni) which suggests that the adaptation to specific environmental conditions have been associated with different morphological features of bulbs. The height followed by the diameter of bulbs has a significant contribution to the achievement of theirweight
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