697 research outputs found

    Heterochromatin protein 1 (HP1) is associated with induced gene expression in Drosophila euchromatin

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    Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein, which is involved in heterochromatin formation and gene silencing in many organisms. In addition, it has been shown that HP1 is also involved in telomere capping in Drosophila. Here, we show a novel striking feature of this protein demonstrating its involvement in the activation of several euchromatic genes in Drosophila. By immunostaining experiments using an HP1 antibody, we found that HP1 is associated with developmental and heat shock–induced puffs on polytene chromosomes. Because the puffs are the cytological phenotype of intense gene activity, we did a detailed analysis of the heat shock–induced expression of the HSP70 encoding gene in larvae with different doses of HP1 and found that HP1 is positively involved in Hsp70 gene activity. These data significantly broaden the current views of the roles of HP1 in vivo by demonstrating that this protein has multifunctional roles

    ADHD and illegal conduct: a survey in Juvenile Justice Services in Puglia

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    Obiettivo. Obiettivo del nostro studio è stato quello di individuare possibili fattori psicopatologici correlati alla messa in atto di condotte antigiuridiche da parte di minori al loro primo reato, nonché eventuali altre variabili individuali, familiari, economiche e socioculturali correlate al fenomeno indagato. Materiali e metodi. È stato condotto uno studio longitudinale su minori della Regione Puglia (Distretto della Corte di Appello di Bari e Lecce) al loro primo reato. Lo studio ha comportato l’acquisizione di una serie di informazioni anagrafiche, familiari, scolastiche, comportamentali e cliniche dei minori arruolati lungo un arco di tempo di oltre un anno e seguiti in follow-up per 18 mesi. Per le sue finalità lo studio prevedeva l’utilizzo di due scale cliniche standardizzate (Youth Self-Report e Conners Adolescent Self Report Scale). Risultati. L’indagine ha fatto emergere alcune variabili psicologiche e psicopatologiche correlate alle condotte antisociali di minori al loro primo contatto con l’Autorità Giudiziaria, nonché altre familiari, sociali e scolastiche. Conclusioni. I risultati ottenuti confermano la necessità di una prevenzione primaria e secondaria su minori a rischio, attraverso interventi multidisciplinari, precoci, selettivi

    Heterochromatin Protein 1 (HP1a) Positively Regulates Euchromatic Gene Expression through RNA Transcript Association and Interaction with hnRNPs in Drosophila

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    Heterochromatin Protein 1 (HP1a) is a well-known conserved protein involved in heterochromatin formation and gene silencing in different species including humans. A general model has been proposed for heterochromatin formation and epigenetic gene silencing in different species that implies an essential role for HP1a. According to the model, histone methyltransferase enzymes (HMTases) methylate the histone H3 at lysine 9 (H3K9me), creating selective binding sites for itself and the chromodomain of HP1a. This complex is thought to form a higher order chromatin state that represses gene activity. It has also been found that HP1a plays a role in telomere capping. Surprisingly, recent studies have shown that HP1a is present at many euchromatic sites along polytene chromosomes of Drosophila melanogaster, including the developmental and heat-shock-induced puffs, and that this protein can be removed from these sites by in vivo RNase treatment, thus suggesting an association of HP1a with the transcripts of many active genes. To test this suggestion, we performed an extensive screening by RIP-chip assay (RNA–immunoprecipitation on microarrays), and we found that HP1a is associated with transcripts of more than one hundred euchromatic genes. An expression analysis in HP1a mutants shows that HP1a is required for positive regulation of these genes. Cytogenetic and molecular assays show that HP1a also interacts with the well known proteins DDP1, HRB87F, and PEP, which belong to different classes of heterogeneous nuclear ribonucleoproteins (hnRNPs) involved in RNA processing. Surprisingly, we found that all these hnRNP proteins also bind heterochromatin and are dominant suppressors of position effect variegation. Together, our data show novel and unexpected functions for HP1a and hnRNPs proteins. All these proteins are in fact involved both in RNA transcript processing and in heterochromatin formation. This suggests that, in general, similar epigenetic mechanisms have a significant role on both RNA and heterochromatin metabolisms

    Transverse momentum spectra of b jets in pPb collisions at sqrt(s[NN]) = 5.02 TeV

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    We present a measurement of b jet transverse momentum (pt) spectra in proton-lead (pPb) collisions using a dataset corresponding to about 35 inverse nanobarns collected with the CMS detector at the LHC. Jets from b quark fragmentation are found by exploiting the long lifetime of hadrons containing a b quark through tagging methods using distributions of the secondary vertex mass and displacement. Extracted cross sections for b jets are scaled by the effective number of nucleon-nucleon collisions and are compared to a reference obtained from PYTHIA simulations of pp collisions. The PYTHIA-based estimate of the nuclear modification factor is found to be 1.22 +/- 0.15 (stat + syst pPb) +/- 0.27 (syst PYTHIA) averaged over all jets with pt between 55 and 400 GeV/c and with abs(eta[lab]) < 2. We also compare this result to predictions from models using perturbative calculations in quantum chromodynamics

    Searches for electroweak production of charginos, neutralinos, and sleptons decaying to leptons and W, Z, and Higgs bosons in pp collisions at 8 TeV

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    Searches for the direct electroweak production of supersymmetric charginos, neutralinos, and sleptons in a variety of signatures with leptons and W, Z, and Higgs bosons are presented. Results are based on a sample of proton-proton collision data collected at center-of-mass energy sqrt(s) = 8 TeV with the CMS detector in 2012, corresponding to an integrated luminosity of 19.5 inverse femtobarns. The observed event rates are in agreement with expectations from the standard model. These results probe charginos and neutralinos with masses up to 720 GeV, and sleptons up to 260 GeV, depending on the model details

    Search for the associated production of the Higgs boson with a top-quark pair

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    A search for the standard model Higgs boson produced in association with a top-quark pair (t tbar H) is presented, using data samples corresponding to integrated luminosities of up to 5.1 inverse femtobarns and 19.7 inverse femtobarns collected in pp collisions at center-of-mass energies of 7 TeV and 8 TeV respectively. The search is based on the following signatures of the Higgs boson decay: H to hadrons, H to photons, and H to leptons. The results are characterized by an observed t tbar H signal strength relative to the standard model cross section, mu = sigma/sigma[SM], under the assumption that the Higgs boson decays as expected in the standard model. The best fit value is mu = 2.8 +/- 1.0 for a Higgs boson mass of 125.6 GeV

    Search for quark contact interactions and extra spatial dimensions using dijet angular distributions in proton-proton collisions at sqrt(s) = 8 TeV

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    A search is presented for quark contact interactions and extra spatial dimensions in proton-proton collisions at sqrt(s) = 8 TeV using dijet angular distributions. The search is based on a data set corresponding to an integrated luminosity of 19.7 inverse femtobarns collected by the CMS detector at the CERN LHC. Dijet angular distributions are found to be in agreement with the perturbative QCD predictions that include electroweak corrections. Limits on the contact interaction scale from a variety of models at next-to-leading order in QCD corrections are obtained. A benchmark model in which only left-handed quarks participate is excluded up to a scale of 9.0 (11.7) TeV for destructive (constructive) interference at 95% confidence level. Lower limits between 5.9 and 8.4 TeV on the scale of virtual graviton exchange are extracted for the Arkani-Hamed--Dimopoulos--Dvali model of extra spatial dimensions

    Differential cross section measurements for the production of a W boson in association with jets in proton-proton collisions at sqrt(s) = 7 TeV

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    Measurements are reported of differential cross sections for the production of a W boson, which decays into a muon and a neutrino, in association with jets, as a function of several variables, including the transverse momenta (pt) and pseudorapidities of the four leading jets, the scalar sum of jet transverse momenta (HT), and the difference in azimuthal angle between the directions of each jet and the muon. The data sample of pp collisions at a centre-of-mass energy of 7 TeV was collected with the CMS detector at the LHC and corresponds to an integrated luminosity of 5.0 inverse femtobarns. The measured cross sections are compared to predictions from Monte Carlo generators, MADGRAPH + PYTHIA and SHERPA, and to next-to-leading-order calculations from BLACKHAT + SHERPA. The differential cross sections are found to be in agreement with the predictions, apart from the pt distributions of the leading jets at high pt values, the distributions of the HT at high-HT and low jet multiplicity, and the distribution of the difference in azimuthal angle between the leading jet and the muon at low values

    Performance of photon reconstruction and identification with the CMS detector in proton-proton collisions at sqrt(s) = 8 TeV

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    A description is provided of the performance of the CMS detector for photon reconstruction and identification in proton-proton collisions at a centre-of-mass energy of 8 TeV at the CERN LHC. Details are given on the reconstruction of photons from energy deposits in the electromagnetic calorimeter (ECAL) and the extraction of photon energy estimates. The reconstruction of electron tracks from photons that convert to electrons in the CMS tracker is also described, as is the optimization of the photon energy reconstruction and its accurate modelling in simulation, in the analysis of the Higgs boson decay into two photons. In the barrel section of the ECAL, an energy resolution of about 1% is achieved for unconverted or late-converting photons from H to gamma gamma decays. Different photon identification methods are discussed and their corresponding selection efficiencies in data are compared with those found in simulated events
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