18 research outputs found

    Different response to epidermal growth factor of hepatocytes in cultures isolated from male or female rat liver. Inhibitor effect of estrogen on binding and mitogenic effect of epidermal growth factor

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    Deoxyribonucleic acid (DNA) synthesis in hepatocytes isolated from the livers of male and female rats has been compared in monolayer culture. Plating efficiency, DNA and protein content, viability, and morphologic appearance were the same in cultures prepared with hepatocytes isolated from male or female rats. Epidermal growth factor (EGF)-induced DNA synthesis was significantly higher in hepatocytes from male rats than in hepatocytes from female rats. This was the case whether hepatocytes were isolated from normal or partially hepatectomized male or female rats. Hepatocytes isolated from regenerating liver synthesize more DNA than those isolated from normal liver in response to EGF. This increased response to EGF in hepatocytes derived from regenerating liver was relatively the same for male- and female-derived hepatocytes, but the magnitude of the response was considerably higher in male-derived hepatocytes. In contrast, in vivo DNA synthesis in the liver remnant after partial hepatectomy was similar in male and female rats if measured 24 h after the operation. A comparison of EGF binding to male- and female-derived hepatocytes maintained in primary culture indicated a lower number of high-affinity receptors for EGF in the female hepatocytes. The addition of estrogen to primary cultures of hepatocytes isolated from male rats inhibited EGF binding as well as EGF-induced DNA synthesis. Our studies show significant differences in DNA synthesis in response to EGF when male and female hepatocytes are compared in primary culture. The regenerative response after partial hepatectomy, on the other hand, was the same in male and female rats. Thus, our studies indicate that the sex of the donor rat is important when hepatocytes in culture are used for a variety of studies, such as hepatocyte metabolism, induction and control of DNA synthesis, and hepatocarcinogenesis. In addition, our results indicate that caution is advised when inferences are made from in vitro findings for in vivo conditions. © 1987

    Effects of Socio-Cultural Level (SCL) on personality dimensions

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    Gli studi sugli effetti del Livello Socio Culturale (LSC) sui fattori di personalitĂ  Big Five hanno sempre indagato Status Socio-Economico ma non Capitale Culturale (CC) e Capitale Sociale (CS). Nel presente lavoro, abbiamo studiato gli effetti di tutte le dimensioni del LSC sulla personalitĂ  di 121 donne e 106 uomini, della stessa area geografica, e li abbiamo confrontati con quelli osservati in precedenza nei loro figli adolescenti. Sono emersi effetti distinti per genere ed etĂ : la personalitĂ  risultata influenzata dal CC in tutte le donne, dal CS nei maschi adolescenti e dal livello di istruzione negli uomini adulti

    Estudo radiográfico das alterações da coluna cervical na artrite reumatóide e sua associação com sinais e sintomas da doença Radiographic study of cervical spine alterations and its clinical correlation in patients with reumathoid arthritis

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    As alterações radiográficas da coluna cervical de 81 pacientes em tratamento ambulatorial de artrite reumatóide foram estudadas e correlacionadas com dor a cervical e os sinais neurológicos relacionados com a compressão das estruturas nervosas contidas no canal vertebral. A correlação das alterações radiográficas com a classificação em graus e em estágios da doença preconizados pela ARA (American Rheumatism Association) e classificação da capacidade funcional de Steinbroker foi também realizada. A avaliação radiográfica foi realizada por meio de radiografias em AP, perfil e dinâmicas da coluna cervical. As alterações radiográficas (migração superior do processo odontóide, instabilidade atlanto-axial, instabilidade sub-axial ou erosão do processo odontóide) foram observada em 65 (80,2%) dos pacientes. Foi observado correlação estatisticamente significativa entre a capacidade funcional dos pacientes (classificação de Steibroker) e estágios da doença (ARA ) com as alterações radiográficas da coluna cervical dos pacientes. A presença de alterações radiográficas da coluna cervical dos pacientes com artrite reumatóide não apresentou correlação estatisticamente significativa com a dor ou sinais de compressão nervosa.<br>The changes in the cervical spine of patients with reumathoid arthritis were studied in 81 patients. The aim of the study was to determine the presence of changes in the cervical spine of these patients and their association with clinical signs, symptoms, stage as recommended by American Rheumatism Association (ARA) and classification of functional status.Odontoid upward migration, sub-axial instability and erosion of the odontoid process were evaluated by conventional plane Rx. Changes of the cervical spine were observed in 68 (83,9%) patients. Significant association (p<0,05) were observed between the classification of functional states and stages as recommended by ARA with radiological changes in the cervical spine. There was no statistical positive correlation between pain and neurological symptoms with the radiographic changes of the cervical spine
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