1,741 research outputs found

    Cultivo de c茅lulas Schwann y efecto de la neurregulina sobre el mismo

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    Las C茅lulas de Schwann forman parte del tejido glial del sistema nervioso perif茅rico. Su principal funci贸n es la participaci贸n en la regeneraci贸n del mimo cuando ha sufrido una lesi贸n. Por ello,es importante llevar a cabo estudios para perfeccionar la regeneraci贸n de las mismas in vitro, aumentando su rendimiento para su posterior aplicaci贸n en la terap茅utica de enfermedades que afectan al adulto. Llevamos a cabo el cultivo de c茅lulas de Schwann procedentes del Nervio Ci谩tico de una rata Wistar, previamente sometidas a un fen贸meno de pre-degeneraci贸n administrando neurregulina. Podemos observar como la neurregulina aumenta la proliferaci贸n y por tanto el rendimiento de los cultivos de c茅lulas de Schwann en los que se administr贸 la misma y como estas c茅lulas mantienen sus caracter铆sticas t铆picas. De la misma manera, pudimos observar como la neurregulina facilitaba el crecimiento de las c茅lulas de Schwann, pero no de otros tipos celulares como los fibroblastos, y por tanto aumentaba la riqueza de los cultivos.Biolog铆a Celular, Histolog铆a y Farmacolog铆aM谩ster en Investigaci贸n en Ciencias de la Salu

    La atrofia cerebral como predictor de reperfusi贸n endovascular f煤til en el infarto cerebral agudo de circulaci贸n anterior

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    Objetivos: 1) Investigar el impacto pron贸stico de la atrofia cerebral sobre el riesgo de RF en pacientes con ictus isqu茅mico agudo tratados TEV. 2) Investigar si existe interacci贸n entre el grado de atrofia y el volumen del infarto en la determinaci贸n del riesgo de RF. 3) Investigar si existe interacci贸n entre la atrofia y la edad en la determinaci贸n del riesgo de RF. 4)Investigar si existe asociaci贸n entre la atrofia y variables pron贸sticas intermedias tras TEV. M茅todos: se estudiaron de forma consecutiva los pacientes con ictus isqu茅mico agudo con oclusi贸n arterial intracraneal de territorio anterior tratados mediante TEV. La atrofia cerebral fue valorada en el TC basal mediante la escala GCA y el 铆ndice de Evans. El volumen del infarto fue evaluado en el TC de control. La RF fue la principal variable. Interacciones de la atrofia con la edad y el volumen del infarto fueron estudiadas con modelos regresi贸n.Departamento de Medicina, Dermatolog铆a y Toxicolog铆aDoctorado en Investigaci贸n Biom茅dic

    The frequency of rehospitalization and associated factors in Colombian psychiatric patients: a cohort study

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    BACKGROUND: The rehospitalization of patients with mental disorders is common, with rehospitalization rates of up to 80% observed in these patients. This phenomenon negatively impacts families, patients, and the health care system. Several factors have been associated with an increased likelihood of rehospitalization. This study was aimed at determining the frequency and the factors associated with rehospitalization in a psychiatric clinic. METHODS: We performed a prospective cohort study with 361 patients who were hospitalized at the Clinic of Our Lady of Peace in Bogota, Colombia from August-December 2009. We calculated the incidence rates of rehospitalization and the risk factors using Cox regression. RESULTS: Overall, 60% of the patients in this cohort were rehospitalized during the year that followed the index event. The variables associated with rehospitalization were separated, divorced, or single status; higher socio-economic strata; a longer duration of index hospitalization; and a diagnosis of substance abuse, schizophrenia, bipolar disorder, or depression. CONCLUSIONS: The rehospitalization rate in our study was as high as reported in other studies. The associated factors with it in this group, may contribute to the design of programs that will reduce the frequency of rehospitalization among patients with mental disorders, in countries like Colombia. Additionally, these results may be useful in interventions, such as coping skills training, psycho-education, and community care strategies, which have been demonstrated to reduce the frequency of rehospitalization

    Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment

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    This manuscript reports an effective new alternative for the management of bone infection by the 5 development of an antibiotic nanocarrier able to penetrate bacterial biofilm, thus enhancing antimicrobial effectiveness. This nanosystem, also denoted as 鈥渘anoantibiotic鈥, consists in mesoporous silica nanoparticles (MSNs) loaded with an antimicrobial agent (levofloxacin, LEVO) 10 and externally functionalized with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as targeting agent. This amine functionalization provides MSNs of positive charges, which improves the affinity towards the negatively charged bacteria wall and biofilm. Physical and 15 chemical properties of the nanoantibiotic were studied using different characterization techniques, including Xray diffraction (XRD), transmission electron microscopy (TEM), N2 adsorption porosimetry, elemental chemical analysis, dynamic light scattering (DLS), zeta (锟 )-potential 20 and solid-state nuclear magnetic resonance (NMR). 鈥淚n vial鈥 LEVO release profiles and the in vitro antimicrobial effectiveness of the different released doses were investigated. The efficacy of the nanoantibiotic against a S. aureus biofilm was also determined, showing the practically total 25 destruction of the biofilmdue to the high penetration ability of the developed nanosystem. These findings open up promising expectations in the field of bone infection treatment

    Ange铆tis granulomatosa del sistema nervioso central. Informe de autopsia

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    The medical history and post-mortem findings of a 29-year-old male who was admitted to the Neurology Department due to headache and bilateral amaurosis are reported. His evolution was torpid, with sustained headache, behavioral changes, visual hallucinations and psychomotor agitation. The patient died at 18 days of hospital stay. At necropsy, it was found a granulomatous angiitis of the central nervous system with extensive hemorrhagic infarcts in the bilateral temporo-occipital lobes and the brainstem associated with severe cerebral edema.Se presentaron la historia cl铆nica y los hallazgos post-mortem de un var贸n de 29 a帽os de edad que fue admitido en el Servicio de Neurolog铆a por cefalea y amaurosis bilateral. Su evoluci贸n fue t贸rpida, con cefalea mantenida, alteraciones conductuales, alucinaciones visuales y excitaci贸n psicomotriz; falleci贸 a los 18 d铆as de estad铆a hospitalaria. En la necropsia realizada se comprob贸 una ange铆tis granulomatosa del sistema nervioso central con infartos hemorr谩gicos extensos en los l贸bulos temporo-occipitales bilaterales y el tallo encef谩lico asociado a edema cerebral severo

    La escritura de rese帽as una estrategia para el desarrollo del pensamiento cr铆tico

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    78 P谩ginas.Este trabajo, se centra en el an谩lisis de la incidencia de la elaboraci贸n de rese帽as literarias en el desarrollo de habilidades del Pensamiento Cr铆tico. Lo anterior, en el 谩mbito del estudiantado de la formaci贸n inicial de maestros de la Escuela Normal Superior Mar铆a Auxiliadora de Villapinz贸n, Cundinamarca. El trabajo incluye, entre otros elementos un amplio y detallado diagn贸stico de los desempe帽os que caracterizan el Pensamiento Cr铆tico de las estudiantes con quienes se trabaj贸, as铆 como una relaci贸n clara entre lo expuesto en el plano te贸rico, con los datos producto de la propuesta de trabajo en el aula, lo cual permiti贸 un completo an谩lisis de estos 煤ltimos. El trabajo se basa principalmente, en la reivindicaci贸n del maestro y su formaci贸n inicial, explorando una estrategia metodol贸gica, orientada a responder a la necesidad de forjar herramientas que ayuden a cumplir con la responsabilidad de formar personas cr铆ticas. Es entonces valioso proponer la utilizaci贸n de la rese帽a literaria y valorar sus alcances en la formaci贸n inicial de maestros

    Documentaci贸n del Sistema de Gesti贸n de Calidad de un laboratorio de una empresa de inyecci贸n de pl谩sticos seg煤n la norma UNE-EN-ISO 17025:2005

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    El objeto del proyecto es documentar el Sistema de Gesti贸n de Calidad de un laboratorio de ensayos ubicado en una PYME tipo, PLASTICAUTO, dedicada a la fabricaci贸n mediante la tecnolog铆a de inyecci贸n, de piezas de pl谩stico para el sector de la Automoci贸n. Las actividades de ensayo que el laboratorio realiza est谩n destinadas a la validaci贸n y control de calidad de las piezas que PLASTICAUTO suministra a sus clientes, as铆 como al control de recepci贸n de las materias primas necesarias para su fabricaci贸n. Este proyecto incluir谩 el Manual de Gesti贸n del Laboratorio y sus Pol铆ticas, Procedimientos Generales, Instrucciones T茅cnicas, Registros y otros documentos necesarios para satisfacer tanto los requisitos organizativos como los requisitos t茅cnicos contemplados en la norma UNE-EN ISO/IEC 17025:2005

    Low-risk polycythemia vera treated with phlebotomies: clinical characteristics, hematologic control and complications in 453 patients from the Spanish Registry of Polycythemia Vera

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    Myelofibrosis; Phlebotomies; ThrombosisMielofibrosi; Flebotomies; TrombosiMielofibrosis; Flebotom铆as; TrombosisHematological control, incidence of complications, and need for cytoreduction were studied in 453 patients with low-risk polycythemia vera (PV) treated with phlebotomies alone. Median hematocrit value decreased from 54% at diagnosis to 45% at 12 months, and adequate hematocrit control over time (鈥60 years, and microvascular symptoms constituted the main indications for starting cytoreduction. Median duration without initiating cytoreduction was significantly longer in patients younger than 50 years (<鈥0.0001). The incidence rate of thrombosis under phlebotomies alone was 0.8% per year and the estimated probability of thrombosis at 10 years was 8.5%. The probability of arterial thrombosis was significantly higher in patients with arterial hypertension whereas there was a trend to higher risk of venous thrombosis in cases with high JAK2V617F allele burden. Rates of major bleeding and second primary neoplasm were low. With a median follow-up of 9 years, survival probability at 10 years was 97%, whereas the probability of myelofibrosis at 10 and 20 years was 7% and 20%, respectively. Progression to acute myeloid leukemia was documented in 3 cases (1%). Current management of low-risk PV patients is associated with low rate of thrombosis and long survival. New treatment strategies are needed for improving hematological control and, in the long term, reducing progression to myelofibrosis.This work was supported by PI18/01472, PI18/00205, and PI21/00231 from the Instituto de Salud Carlos III (ISCIII), through the Plan Estatal de Investigaci贸n Cient铆fica y T茅cnica y de Innovaci贸n. GEMFIN received a grant from Novartis for the development of the Spanish Registry of Polycythemia Vera and for conducting the present project

    Mesoporous Silica Nanoparticles Decorated with Polycationic Dendrimers for Infection Treatment

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    This work aims to provide an effective and novel solution for the treatment of infection by using nanovehicles loaded with antibiotics capable of penetrating the bacterial wall, thus increasing the antimicrobial effectiveness. These nanosystems, named "nanoantibiotics", are composed of mesoporous silica nanoparticles (MSNs), which act as nanocarriers of an antimicrobial agent (levofloxacin, LEVO) localized inside the mesopores. To provide the nanosystem of bacterial membrane interaction capability, a polycationic dendrimer, concretely the poly(propyleneimine) dendrimer of third generation (G3), was covalently grafted to the external surface of the LEVOloaded MSNs. After physicochemical characterization of this nanoantibiotic, the release kinetics of LEVO and the antimicrobial efficacy of each released dosage were evaluated. Besides, internalization studies of the MSNs functionalized with the G3 dendrimer were carried out, showing a high penetrability throughout Gram-negative bacterial membranes. This work evidences that the synergistic combination of polycationic dendrimers as bacterial membrane permeabilization agents with LEVO-loaded MSNs triggers an efficient antimicrobial effect on Gram-negative bacterial biofilm. These positive results open up very promising expectations for their potential application in new infection therapies

    Caffeinating the biofuels market:Effect of the processing conditions during the production of biofuels and high-value chemicals by hydrothermal treatment of residual coffee pulp

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    5 figures, 4 tables, supplementary information.-- 漏 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/The manufacturing of coffee, one of the most popular beverages globally, renders enormous amounts of by-products and wastes, which may trigger severe environmental issues if not treated appropriately. The coffee pulp, resulting from the wet processing of coffee, is the predominant by-product, with around 10 Mt annually produced worldwide. For the first time, this work addresses the hydrothermal treatment of coffee pulp to produce biofuels and platform molecules, scrutinising the influence of the processing conditions (temperature, pressure, reaction time and solid/water ratio) on the process. This strategy allowed the transformation of coffee pulp into bio-crude and hydrochar in different yields (10鈥26% and 10鈥42%, respectively), depending on the conditions. The bio-crude included a pool of alkanes, carboxylic acids, ketones, phenols and nitrogen species, with varying quantities of C (54鈥71 wt%), H (6鈥7 wt%), O (18鈥34 wt%) and N (3鈥5 wt%) and a calorific value shifting from 23 to 32 MJ/kg. The hydrochar contained different proportions of C (57鈥72 wt%), H (4鈥6 wt%), O (20鈥35 wt%) and N (2鈥3 wt%) and had a calorific value between 22 and 29 MJ/kg. Process optimisation showed that up to 45% of the coffee pulp could be simultaneously converted into energy-rich (29 MJ/kg), merchantable liquid (20% bio-crude) and solid (24% hydrochar) biofuels during the treatment of a 15 wt% coffee pulp suspension at 320 掳C and 162 bar for 1 h. At the same time, a bio-crude with a high proportion of profitable phenolic derivatives (42%) can be attained in high yield (25%) when a 5 wt% suspension is treated at 280 掳C and 120 bar for 2 h. These promising results, along with the bespoke nature of this hydrothermal treatment, are a landmark achievement for the economy and sustainability of coffee producer countries, thus representing a pioneering step change towards the sustainable management of early-stage coffee leftovers.The authors wish to express their gratefulness to FEDER, the Spanish Ministry of Science, Innovation and Universities (Grant Number ENE2017-83854-R) and the Spanish National Research Council (CSIC) (Grant Number COOPA20367) for providing financial support. Besides, Javier Rem贸n is very grateful to the Spanish Ministry of Science, Innovation and Universities for the Juan de la Cierva (JdC) fellowships (Grant Numbers FJCI-2016-30847 and IJC2018-037110-I) awarded. Lorena Pedraza-Segura and Pedro Arcelus-Arrillaga would like to acknowledge the financial support of INIAT and DINV at Universidad Iberoamericana for their research.Peer reviewe
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