1,322 research outputs found

    Ala-504 is a determinant of substrate binding affinity in the mouse Na+/dicarboxylate cotransporter

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    AbstractThe Na+/dicarboxylate cotransporters from mouse (mNaDC1) and rabbit (rbNaDC1) differ in their ability to handle adipate, a six-carbon terminal dicarboxylic acid. The mNaDC1 and rbNaDC1 amino acid sequences are 75% identical. The rbNaDC1 does not transport adipate and only succinate produced inward currents under two-electrode voltage clamp. In contrast, oocytes expressing mNaDC1 had adipate-dependent inward currents that were about 60% of those induced by succinate. In order to identify domains involved in adipate transport, we examined the functional properties of a series of chimeric transporters made between mouse and rabbit NaDC1. We find that multiple transmembrane helices (TM), particularly TM 8, 9, and 10, are involved in adipate transport. In TM 10 there is only one amino acid difference between the two proteins, corresponding to Ala-504 in mouse and Ser-512 in rabbit NaDC1. The mNaDC1-A504S mutant had decreased adipate-dependent currents relative to succinate-dependent currents and an increase in the K0.5 for both succinate and glutarate. We conclude that multiple amino acids from TM 8, 9 and 10 contribute to the transport of adipate in NaDC1. Furthermore, Ala-504 in TM 10 is an important determinant of K0.5 for both adipate and succinate

    Pregnancy Outcome in Swiss-Webster Mice Infected With Chlamydia trachomatis

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    Objective: The objective of this study was to observe pregnancy outcomes in mice infected transvaginally with Chlamydia trachomatis

    Análise da savana e queimadas no Parque Indígena de Tumucumaque (PA) através de imagens de satélite Landsat.

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    The Tiriyós Savanna, inserted in the Indian Park of Tumucumaque (PA), and the occurrence of fires were analyzed using satellite images. The savanna common and permanent area in the years of 1986 and 2005 was 4.109,72 km2. The total retraction and expansion areas in the same years were 287,3 km2 and 151,8 km2, respectively. From the burnt area, 38,5% presented only one focus of fire in 9 years (from 1998 August to 2006 October); in 23,6% of the area there was fire each 4 and a half years; 25,3% each 31 months; 10,8% each 18 months and 1,8% each year. The total estimated burnt area was 4.086 km2, at maximum. The fires are considered one of the pressure factors for the advance of the amazon savannas and in the change of the coverage and usage of the earth in the Tiriyós savanna area, but the analysis of the satellite images in the years of 1986 and 2005 showed that there was a retraction of 135,5 km2 in 20 years

    GSK3-mediated raptor phosphorylation supports amino acid-dependent Q2 mTORC1-directed signalling

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    The mammalian or mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) is a ubiquitously expressed multimeric protein kinase complex that integrates nutrient and growth factor signals for the co-ordinated regulation of cellular metabolism and cell growth. Herein, we demonstrate that suppressing the cellular activity of glycogen synthase kinase-3 (GSK3), by use of pharmacological inhibitors or shRNA-mediated gene silencing, results in substantial reduction in amino acid (AA)-regulated mTORC1-directed signalling, as assessed by phosphorylation of multiple downstream mTORC1 targets. We show that GSK3 regulates mTORC1 activity through its ability to phosphorylate the mTOR-associated scaffold protein raptor (regulatory-associated protein of mTOR) on Ser(859). We further demonstrate that either GSK3 inhibition or expression of a S859A mutated raptor leads to reduced interaction between mTOR and raptor and under these circumstances, irrespective of AA availability, there is a consequential loss in phosphorylation of mTOR substrates, such as p70S6K1 (ribosomal S6 kinase 1) and uncoordinated-51-like kinase (ULK1), which results in increased autophagic flux and reduced cellular proliferation

    Espacialização da pecuária de corte sul-mato-grossense.

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    A economia do Estado de Mato Grosso do Sul baseia-se principalmente na agricultura e pecuária, e o estado é subdivido em quatro mesorregiões: Centro-Norte, Pantanal, Sudoeste e Leste. Para espacializar a produção da pecuária bovina sul-mato-grossense, foram usados dados censitários e geocódigos municipais do IBGE e procedimentos do sistema de informação geográfica (SIG). As mesorregiões Leste e Sudoeste comportam o maior número de cabeças, da ordem de seis milhões. A mesorregião do Pantanal possui imensas áreas alagadas e favorece a exploração extensiva, e a mesorregião Centro-Norte, localizada mais próxima aos grandes mercados consumidores, têm cerca de quatro milhões de cabeças. A taxa geométrica de crescimento (TGC) do rebanho bovino de 2006 a 2011 apresenta padrão irregular, com variação de -6,86 a +7,31, -5,60 a +13,33, -2,65 a +6,65 e -5,15 a +2,45 para as regiões Pantanal, Centro-Norte, Sudoeste e Leste, respectivamente. Em termos nacionais, as projeções da TGC (2007 a 2023) para consumo e exportação são da ordem de 30% e 22,4%, respectivamente, enquanto a TGC para produção nesse mesmo período é da ordem de 18,4%. Esses números representam menos oferta interna e potencial aquecimento dos preços domésticos, contribuindo, assim, para um possível aumento da taxa de inflação no período analisado
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