19 research outputs found

    Detection of IL-1β-induced nuclear translocation of NF-κB by immunofluorescence in C3842 cells (A–D) and SW1353 cells (E-H).

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    <p>In untreated cells and cells treated with Curcumin or Curcumin+IL-1β, NF-κB was detected in the cytoplasm, not in the nucleus (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0099296#pone-0099296-g006" target="_blank">Figure 6</a>). In cells treated with IL-1β nuclear translocation of NF-κB was evident.</p

    Relative expression of genes associated with NF-κB. RNA was extracted from C3842 cells treated with IL-1β (10 ng/ml), Curcumin (20 µmol/l) or both.

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    <p>qRT-PCR was performed using the NF-κB primer library (RealTimePrimers, Elkins Park, PA, USA).</p><p>See Material and Methods section for details. The gene expression levels were normalized to the expression of ribosomal protein 13a (RPL13a). The Curcumin effect on IL-1β-induced gene expression was calculated as ratio of the gene expression in cells treated with IL-1β+Curcumin and cells treated with IL-1β alone. Relative changes higher than 2-fold are marked bold. Relative changes lower than 0.5-fold are marked italic. Putative housekeeping genes other than RPL13a are indicated by italics.</p

    Effect of Curcumin on VEGF-A expression.

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    <p>IL-1β-induced VEGF-A expression is blocked in C3842 and SW1353 chondrosarcoma cells after incubation with Curcumin.</p

    Illustration of the data presented in table 1, representing the number of genes induced (green) or repressed (red) by treatment with IL-1β, Curcumin or IL-1β+Curcumin.

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    <p>The size of the circles is proportional to the number of genes in each group. The threshold was set over 2 and under 0.5-fold variation in gene expression. Additionally, the Curcumin effect indicating reduced expression is indicated. Numbers in overlaps indicate the number of genes that shared in the groups.</p

    Angiogenesis assay with quantitative image analysis.

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    <p>Cell culture supernatants from C3842 cells treated with IL-1β led to increased number and length of microvessel segments (D). Treatment with Curcumin blocked IL-1β-induced tube formation (F). Controls with supernatants from untreated cells (C) and cells treated with Curcumin (E) were included. Control with FCS-free medium (A). Control with medium supplemented with FCCS (B).</p

    An appropriate concentration of Curcumin is necessary for the inhibitory effect on IL-1 signaling.

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    <p>At least a Curcumin concentration of 15 µmol/l is required to block IκBα phosphorylation in C3842 (A) and SW1353 (B) chondrosarcoma cells.</p