15 research outputs found

    summary_statistics

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    This file contains the details about the number of raw reads and the mapping statistics such as uniquely mapped reads, mapped to multiple loci and unmapped reads for all the samples

    Differentially expressed genes

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    Each spread sheet contains the list of differentially expressed genes (FDR <5%) at each time point and concentration

    KEGG and Gene Ontology Pathway analysis of the downregulated genes in the brain

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    This file contains the list of genes associated with each enriched KEGG and GO terms in the whole brain samples of 0.3nM and 1.2nM PCB126 exposed adult fish. These GO and KEGG terms are enriched in the downregulated gene set

    Common genes

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    This is the list of genes that are common to both 0.3 nM and 1.2 nM PCB126 exposed adult whole brain samples

    KEGG and Geno Ontology Pathway analysis of upregulated genes in the brain

    No full text
    This file contains the list of genes that are represented under each enriched GO and KEGG pathway terms. These genes are differentially expressed in the adult brain samples exposed to either 0.3nM or 1.2nM PCB126

    GSE98741

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    Strand-specific RNAsequencing data from zebrafish embryos, larvae and adult brain tissue developmentally exposed to two different concentrations of PCB126. Raw data files (fastq) were pre-processed, reads were mapped to the zebrafish genome (GRCz10; ensembl version 87) using STAR aligner. Read counts were obtained using HTSeq-count and differential gene expression analysis was done using edgeR

    Common genes

    No full text
    This is the list of genes that are common to both 0.3 nM and 1.2 nM PCB126 exposed adult whole brain samples

    Impact of bisphenol A (BPA) on survival and growth.

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    <p>(A) Percent survival of trout embryos was calculated at the end of the experimental period (400-dpf). (B) Temporal changes in average body mass (g), measured every two weeks after the time of first feed (65 dpf), in the control and BPA exposed groups during development, and (C) in juveniles at 400 dpf. Oocytes were exposed to either control (vehicle alone) or BPA at 30 µg.ml<sup>−1</sup> (low) or 100 µg.ml<sup>−1</sup> (high) for 3 h and fertilized with untreated sperm. Fertilized eggs were incubated at 8.5°C and were sampled at various time points during development; values represent mean + SEM (n = 6); bars with different letters are statistically significant (two-way ANOVA for temporal changes and one-way ANOVA for single time point; p<0.05).</p

    Impact of bisphenol A (BPA) on growth hormone (GH) content and gene expressions.

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    <p>Temporal changes in GH content in the control and BPA treated groups during early development (A) and plasma GH levels at 400-dpf (B); Temporal changes in GH1 (C) and GH2 (D) mRNA abundances in trout exposed to control and two different concentrations of BPA. Maternal transcript levels were measured in freshly fertilized eggs and represented as 0-dpf (dark colored bar). GH was determined using trout specific anti-GH antibody as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010741#s4" target="_blank">materials and methods</a>. Two-way ANOVA was used to determine the effect of time, treatment and interaction effects on GH levels and transcript levels during development (Bonferonni posthoc test; p<0.05). Different letters represent differences between treatments at each time point. Asterisk (*) represent effect of time on GH content. One way ANOVA was used to determine the effect of BPA on plasma GH levels. All values represent mean + SEM (n = 7).</p

    Impact of bisphenol A (BPA) on insulin-like growth factors (IGFs) and their receptors gene expressions.

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    <p>Temporal changes in IGF-1 (A) and IGF-2 (B) and IGF-1 receptor a (IGF-1 Ra; C) and b (IGF-I Rb; D) mRNA abundances in rainbow trout embryos in response to BPA treatment. See the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010741#pone-0010741-g002" target="_blank">Fig. 2</a> legend for experimental details. Maternal transcript levels were measured in freshly fertilized eggs and represented as 0-dpf (dark colored bar). Two-way ANOVA was used to determine the effect of time, treatment and interaction effects on the transcript levels during development (Bonferonni post hoc test; p<0.05). Different letters represent differences between treatments at each time point. Asterisk (*) represent effect of time on mRNA abundances. All values represent mean + SEM (n = 7).</p
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