2 research outputs found

    The development of ALICE-tRNA-sequencing and its use in exploring the role of tRNAs in translational control

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    Sustaining proliferative signalling and loss of translational control is arguably the most fundamental trait of cancer cells, enabling tumour growth and metastatic dissemination. Transfer RNAs (tRNAs) have long been considered abundant “housekeeping” RNAs, functioning to decipher the universal genetic code. However, exhaustive analyses have implicated tRNA participation in a host of regulatory networks including the cellular stress response and protein synthesis. Recent findings suggest that the expression of tRNAs for synonymous codon usage is dependent on the differentiation/proliferation status of the cell and are coordinated with changes in translation. Although the molecular mechanisms that govern these changes are yet to be elucidated, cellular tRNA composition potentially introduces an additional layer of translational control. tRNAs are the most post-transcriptionally modified RNA species, with well over 50 unique modifications identified in eukaryotes. Consequently, isoacceptor identification and the measuring of the tRNA pool using next generation sequencing has long been an area of interest, with many attempts being made in literature. Using the Escherichia coli dealkylating enzyme AlkB and the novel tRNA high throughput sequencing methodology ALICE-tRNA-seq, we have developed a methodology that can accurately measure relative tRNA pools in vitro and in vivo. We show how other published tRNA sequencing protocols show bias towards tRNA sub populations, with our method showing a more realistic distribution across all tRNAs. We also show relative distribution changes in cellular and genetically modified mouse models of cancer, opening up a high resolution approach to establish the role of tRNAs in translational control and cell fate decisions

    Somali Business Review 2013

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    I t has been more than a year that the last edition of SBR was issued. After an unexpected absence of one year, SBR is here again to share with you very interesting articles enlightening technology, business and development. Our respected readers, we apologize the time that our connection in this beautiful knowledge sharing endeavor has stopped. A new commitment starts now and we connect each other again. We are sure that the upcoming issues of SBR will be more exciting and entertaining Since the last time we have published the last edition, many things in business has changed including both ups and downs. The new opportunities to be grabbed or the arising challenges to be eliminated/ managed and the way we do business are a lot better than ever. To see/understand what it looks like, your experiences, insights and invaluable contributions are extremely important for both academicians and business practitioners. Let’s utilize the venue that SBR provides to all of us by sending your articles through an e-mail or hand delivery to Research and Publications Office at the main camps. Finally, comments and constructive criticism are most welcome, and SBR team will take into consideration immediately. The team’s philosophy is that “The believer is a mirror for his brother believer”. Continues improvement is our target which we believe is possible with a collective ideas and vision