37 research outputs found

    Alignment of coded cpDNA sequences of the Doronicum clusii aggregate (Asteraceae)

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    Alignment (in fasta format) of three concatenated cpDNA regions, coded for TCS analysis, of the Doronicum clusii aggregate (Asteraceae). The three regions are: ndhF-rpl32 intergenic spacer (positions 1-1057); rpl32-trnL(UAG) intergenic spacer (positions 1058-1914); 3'rps16-5'trnK(UUU) intergenic spacer (positions 1915-2776). Sample names have the following format: Taxon name_population number_individual number

    Alignment of original cpDNA sequences of the Doronicum clusii aggregate (Asteraceae)

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    Alignment (in fasta format) of three concatenated cpDNA regions of the Doronicum clusii aggregate (Asteraceae). The three regions are: ndhF-rpl32 intergenic spacer (positions 1-1099); rpl32-trnL(UAG) intergenic spacer (positions 1100-1959); 3'rps16-5'trnK(UUU) intergenic spacer (positions 1960-2883). Sample names have the following format: Taxon name_population number_individual number

    Doronicum_clusii_aggregate_ITS_alignment

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    Alignment (in fasta format) of nuclear ITS sequences of the Doronicum clusii aggregate (Asteraceae). Sample names have the following format: Taxon name_population number_individual number_clone number (clone numbers only for some individuals)

    AFLP matrix for Doronicum clusii aggregate (Asteraceae)

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    AFLP data matrix (as comma separated file) of the Doronicum clusii aggregate (Asteraceae). Sample names have the following format: Taxon name_population number_individual number

    Sequence statistics of studied DNA regions.

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    <p><sup>a</sup> with / without outgroup sequences.</p><p>Sequence statistics of studied DNA regions.</p

    Mitotic chromosomes of members of the <i>Doronicum clusii</i> aggregate.

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    <p>(a) <i>D. clusii</i> s.s. from Mt. Plose, Dolomiten, Italy (pop. 42; CP1042), 2<i>n</i> = 60; (b) <i>D. stiriacum</i> from Mt. H√∂chstein, Schladminger Tauern, Austria (pop. 34; CP1041), 2<i>n</i> = c. 120; (c) <i>D. glaciale</i> subsp. <i>glaciale</i> from Mt. Gruft, Gurktaler Alpen, Austria (pop. 15; CP1038), 2<i>n</i> = 60. Scale bars = 5 őľm.</p

    Overview of the taxa of the <i>Doronicum clusii</i> aggregate and their diagnostic morphological characters (summarized from [50,58,59,113,114,116]).

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    <p>Overview of the taxa of the <i>Doronicum clusii</i> aggregate and their diagnostic morphological characters (summarized from [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref050" target="_blank">50</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref058" target="_blank">58</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref059" target="_blank">59</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref113" target="_blank">113</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref114" target="_blank">114</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118197#pone.0118197.ref116" target="_blank">116</a>]).</p

    Populations of the <i>Doronicum</i> taxa investigated, sampling locations (voucher information), relative fluorescence, number of investigated individuals, AFLP-derived gene diversity and rarity, Prabclus assignments, plastid haplotype, and GenBank accession numbers.

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    <p><sup>a</sup> countries: A, Austria; BIH, Bosnia and Herzegovina; CH, Switzerland; I, Italy; RO, Romania; SK, Slovakia; collectors: PEG, P. Escobar Garc√≠a; AH, RF, R. Flatscher; BF, B. Frajman; CG, C. Gilli, MG, M. Gina; HPG, H. P. Grohmann; AH, A. Hahnekamp; CP, C. Pachschw√∂ll; JP, J. Pachschw√∂ll; GMS, G. M. Schneeweiss, PS, P. Sch√∂nswetter; MS, M. Sonnleitner.</p><p><sup>b</sup> Estimated with flow cytometry for a pooled sample of 3‚Äď6 individuals and given as ratio of DAPI-stained nuclei compared to the internal standards <i>Pisum sativum</i> (not indicated) or <i>Vicia faba</i> (marked with an asterisk).</p><p><sup>c</sup> Number of individuals used for DAPI flow cytometry and AFLP genotyping, respectively.</p><p><sup>d</sup> Plastid DNA haplotypes derived from <i>ndh</i>F-<i>rpl</i>32, <i>rpl</i>32-<i>trn</i>L<sub>UAG</sub> and 3‚Ä≤<i>rps</i>16‚Äď5‚Ä≤<i>trn</i>K<sub>UUU</sub>-sequences.</p><p><sup>e</sup> Assignment to the four Prabclus groups. calc: <i>D. glaciale</i> subsp. <i>calcareum</i>, glac: <i>D. glaciale</i> subsp. <i>glaciale</i>, clus: <i>D. clusii</i> s.s., stir: <i>D. stiriacum</i>: number of individuals (proportion of correct assignment to the original cluster, i.e. bootstrap support of correct assignment; proportion of assignment to additional clusters within the same species, i.e., oversplitting; proportion of no assignment).</p><p><sup>f</sup><i>ndh</i>F-<i>rpl</i>32; <i>rpl</i>32-<i>trn</i>L<sub>UAG</sub>; 3‚Ä≤<i>rps</i>16‚Äď5‚Ä≤<i>trn</i>K<sub>UUU</sub>; ITS uncloned / ITS cloned.</p><p>Populations of the <i>Doronicum</i> taxa investigated, sampling locations (voucher information), relative fluorescence, number of investigated individuals, AFLP-derived gene diversity and rarity, Prabclus assignments, plastid haplotype, and GenBank accession numbers.</p

    Polymorphic sites from the ITS region in the <i>Doronicum clusii</i> aggregate: nucleotides are those found without cloning and (in parentheses) after cloning.

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    <p><sup>a</sup> morphological intermediates between <i>D. glaciale</i> subsp. <i>calcareum</i> and <i>D. glaciale</i> subsp. <i>glaciale</i>.</p><p>Polymorphic sites from the ITS region in the <i>Doronicum clusii</i> aggregate: nucleotides are those found without cloning and (in parentheses) after cloning.</p
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