Massive M2M Access with Reliability Guarantees in LTE Systems

Machine-to-Machine (M2M) communications are one of the major drivers of the cellular network evolution towards 5G systems. One of the key challenges is on how to provide reliability guarantees to each accessing device in a situation in which there is a massive number of almost-simultaneous arrivals from a large set of M2M devices. The existing solutions take a reactive approach in dealing with massive arrivals, such as non-selective barring when a massive arrival event occurs, which implies that the devices cannot get individual reliability guarantees. In this paper we propose a proactive approach, based on a standard operation of the cellular access. The access procedure is divided into two phases, an estimation phase and a serving phase. In the estimation phase the number of arrivals is estimated and this information is used to tune the amount of resources allocated in the serving phase. Our results show that the proactive approach is instrumental in delivering high access reliability to the M2M devices.Comment: Accepted for presentation in ICC 201

Random Access for Machine-Type Communication based on Bloom Filtering

We present a random access method inspired on Bloom filters that is suited for Machine-Type Communications (MTC). Each accessing device sends a \emph{signature} during the contention process. A signature is constructed using the Bloom filtering method and contains information on the device identity and the connection establishment cause. We instantiate the proposed method over the current LTE-A access protocol. However, the method is applicable to a more general class of random access protocols that use preambles or other reservation sequences, as expected to be the case in 5G systems. We show that our method utilizes the system resources more efficiently and achieves significantly lower connection establishment latency in case of synchronous arrivals, compared to the variant of the LTE-A access protocol that is optimized for MTC traffic. A dividend of the proposed method is that it allows the base station (BS) to acquire the device identity and the connection establishment cause already in the initial phase of the connection establishment, thereby enabling their differentiated treatment by the BS.Comment: Accepted for presentation on IEEE Globecom 201

Complete Genome Sequence of the First Colistin-Resistant Raoultella electrica Strain.

We present the full genome sequence of a colistin-resistant Raoultella electrica strain (MIC, >4 μg/mL) that was isolated from the stool of a healthy person living in India. The sequence consists of a chromosome and three plasmids (5,455,992-bp and 98,913-bp, 4,232-bp, and 3,961-bp, respectively). No previously described colistin resistance mechanisms were detected

Genome stability during serial sub-culturing in hyperepidemic multidrug-resistant Klebsiella pneumoniae and Escherichia coli.

BACKGROUND Core-genome single-nucleotide variant (cgSNV) analysis represents a powerful tool for epidemiological investigations of multidrug-resistant (MDR) bacteria. However, cgSNV thresholds to confirm whether isolates are the same clone are not formally defined. METHODS We implemented hybrid whole-genome sequencing to study the genomic changes of 4 MDR isolates belonging to hyperepidemic sequence types (STs) during 20 propagation steps (T20) on MacConkey and CHROMID ESBL plates. The following strains were analyzed: K. pneumoniae AE-2247421 (OXA-48/NDM-1-producing, ST101), K. pneumoniae MCL-2017-2 (CTX-M-15-producing, ST307), E. coli Ec-042 (OXA-181-producing, ST410), and E. coli Ec-050 (NDM-5-producing, ST167). The genome assembly at T5 and T20 was compared to that at time point zero (T0) and to two reference genomes. RESULTS At T20, AE-2247421 lost the IncL blaOXA-48-carrying plasmid when grown on CHROMID ESBL plates, while a large fragment encompassing blaNDM-1 was lost from its IncC plasmid when grown on both plates. In contrast, no structural changes were noted for the other 3 strains. With regard to the cgSNVs, the following results were obtained at T5 and T20 (ranges considering the different agar plates and reference genomes): AE-2247421 (1-8 and 2-12 cgSNVs), MCL-2017-2 (both 1-2 cgSNVs), Ec-042 (both 0 cgSNVs), and Ec-050 (0-6 and 0-9 cgSNVs). CONCLUSIONS We showed that structural changes and accumulation of cgSNVs can occur in few propagation steps under laboratory conditions. These changes might also arise in the clinical context in a short time, especially under antibiotics treatment. This phenomenon should be carefully considered because it might affect the final interpretation of epidemiological genomic analyses