14 research outputs found

    Gambaran Histopatologik Testis Tikus Wistar (Rattus Norvegicus) Setelah Pemberian Monosodium Glutamate (MSG)

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    : Monosodium glutamate (MSG) is a sodium salt from glutamic acid which is currently very popular to be used as a food flavoring ingredient to stimulate appetite. The objective of the study to discover the histologic findings of wistar rats\u27 testicles after MSG administration. This was an experimental laboratoric study, using 20 wistar rats that has been divided into 4 groups. The negative control group is given standard pallet and drinking water for 40 days; it devided to 2 smaller groups, K1 and K2, each of the group was terminated on the 21st and 41st day. The intervention group was given MSG according to average consumption dose in Indonesia; it devided to two smaller groups, P1 and P2, each group was terminated on the 21st and 41st day. The result showed on group K1 and K2, a normal finding of seminiferous tubules, spermatogenic cell layer arrays, and interstitial cell density was found. On group P1, showed seminiferous tubules with decrease of spermatogenic cells development, causing the tubules compartment to appear vacant; the spermatogonia layers appeared sparse on basal membrane, and fewer interstitial cells. On group P2, the findings were not of much difference with group P1, but in this group, one testicle specimen showed calcification cells inside its seminiferous tubules was found. Conclusion: Administration of MSG according to average consumption dose in Indonesia causes decrease of seminiferous tubules\u27 diameter and decrease of the number of spermatogenic cells and interstitial cells

    Pengaruh Pemberian Minyak Kanola Terhadap Gambaran Histopatologik Aorta Dan Kadar Kolesterol Tikus Wistar Dengan Diet Tinggi Lemak

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    : Canola oil is a source of vegetable oils which contains high unsaturated fats that can reduce the risk of coronary heart disease, especially atherosclerosis. One of the trigger factors of atherosclerosis is hypercholesterolemia. This study aimed to evaluate the histopathological features of aorta and the levels of LDL and HDL cholesterols in Wistar rats fed with high fat diet and canola oil. There were 20 Wistar rats in this study, divided into four groups: group A, the negative control; group B with high-fat diet (1 ml lard and 1 ml yolk daily); group C with high-fat diet and 0.1 ml of canola oil daily; and group D with high-fat diet and simvastatin 0.2 mg daily. The results showed elevated levels of LDL and HDL cholesterols in the treated groups compared to the control group. The histopathological features revealed many foam cells in the aorta wall (intima and media layers) of group B, whereas group C had fewer foam cells. No foam cells were found in group D. Conclusion: Although the mean LDL and HDL cholesterol levels of all treated groups were higher than of the control group, canola oil could reduce foam cell formation

    Efek Pemberian Anabolik Androgenik Steroid Injeksi Dosis Rendah Dan Tinggi Terhadap Gambaran Histopatologi Hati Dan Otot Rangka Tikus Wistar (Rattus Novergicus)

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    : Androgenic-anabolic steroids (AAS) are synthetic derivatives of the male hormone endogenous testosterone that stimulates anabolic (protein synthesis) and androgenic effects (masculinization). Long-term USAge of AAS can result in liver damage. However, physiological concentrations of testosterone can stimulate protein synthesis which lead to an increase in muscle size, body mass, and endurance. This study aimed to determine the histopathology of liver and skeletal muscles of wistar rats that were given low dose and high dose injection of AAS. Subjects were 21 wistar rats divided into 7 groups. Group A was given standard pellets for 56 days (negative control), terminated on days 29,43, and 57. Group B was treated with low-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group C was treated with low-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group D was treated with low-dose AAS injection and standard pellets for 56 days, terminated on day 57. Group E was treated with high-dose AAS injection and standard pellets for 28 days, terminated on day 29. Group F was treated with high-dose AAS injection and standard pellets for 42 days, terminated on day 43. Group G was treated with high-dose AAS injection and standard pellets for 56 days, terminated on day 57. The results showed that the histopathology of liver and muscles in group A was still normal. In group B, the architecture of liver was still normal with a few inflammatory cells around the Kiernan triangle while in muscle the ratio of myofibril diameter was 1.28:1. In group C and group D, there were widening of the hepatic artery, bile duct, and portal vein containing blood fibrin, and inflammatory cells around the Kiernan triangle. The ratio of myofibril diameter was 1.43:1 in group C and 2.14:1 in group D. In group E, F and G, there were micro-vesicular fatty cells in the peripheral part of the liver meanwhile the myofibril diameter ratio of the muscles in group E was 1.43:1, group F 2.1:1, and group G 2.28:1. Conclusion: Administration of AAS injection of low dose and high dose for less than 4 weeks could result in inflammation, dilation of the portal vein, hepatic artery and bile duct meanwhile administration of AAS for over 4 weeks could ressult in focal fatty liver (steatosis). The administration of AAS injection of low dose and high dose for 4,6 and 8 weeks reslutid in enlargement of skeletal muscle (muscle hypertrophy)

    Gambaran Histopatologis Lambung Tikus Wistar (Rattus Novergicus) Yang Diberikan Alkohol

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    : Alcohol has become aglobal problem. When it is consumed alcohol can disrupt the structure and function of the digestive tract. Objective: To reveal the histopathological features of the gastric of Wistar rats that are administered with bir, wine, whisky and cap tikus. Method: Experimental research using 15 rats that ware fasted and divided into 5 groups those are group control group which was treated by water, group A which was treated by beer (4.9% alcohol),group B which was treated by wine (14% alcohol), group C which was treated by whiskey (43% alcohol),and group D rats which wastreated by cap tikus(70% alcohol). The treatment of the test animals is done in 5 days. Results:In the control group on mucosa, submucosa, muscularis and serosa layers,inflammatory cells are also seen.In group Ainflammatory cells found on submucosal layer, intestinal metaplasia, and dilation of blood vessels. In group B inflammatory cell, intestinal metaplasia and dilation of blood vessels are found.In group C erosion, inflammatory cells, intestinal metaplasia and dilation of blood vessels are found.In group D submucosal necrosis, inflammatory cells, intestinal metaplasia and hyperemia are found. Conclusions: The provision of alcoholic drinks (beer, wine, whiskey, and cap tikus) in wistar can cause acute gastritis and of the high alcohol content (70% alcohol content)will be accompanied by gastric necrosis

    Pengaruh Pemberian Ekstrak Kunyit Terhadap Gambaran Histopatologi Aorta Tikus Wistar (Rattus Novergicus) Hiperlipidemia

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    : Atherosclerosis is a chronic inflammatory process that can be triggered by one of the risk factors of hyperlipidemia. This research was conducted to see the aorta associated with the provision of margarine that induce hyperlipidemia and administration of curcumin turmeric-containing compounds that act as antioxidants and anti-inflammatory. This research is experimental. The purpose of this study to determine the effect of extract of turmericon Wistar rat aorta histopathology induced hyperlipidemia with margarine. This study used 20 Wistar rats were divided into 5 groups, each group consisting of 4 rats. Group A (negative control group) is given only standard pellets, group B (positive control group) was given margarine 5gr/head /dayfor 28 days, group C was given margarine 5gr/head /day for 28 days followed by administration of standard pellets for 7 day, group D given margarine 5gr/head/day for 28 days followed by administration of turmeric extract 50 mg /head/day for 7 days, and group E are given margarine 5gr/head/day along with turmeric extract 50 mg/head/dayfor 28days. The results shows that microscopically visible aortic wall with foam cells in the tunica intima and tunica media in the positive control group and the administration of 50 mg of turmeric extract reduced foam cells. Conclusion: Turmeric extract showed foam cells in the intima and media less than that of margarine

    Gambaran Histopatologi Hati Tikus Wistar Yang Diberikan Boraks

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    : Borax is an ingredient that is widely used for industrial or antiseptic cleaning agents that are toxic, but borax is used as an additive ind foods and any experience accumulated in the liver that can cause liver disfumction. Methods: The aim of research is to see macroscopic and microscopic of live wistar rats were given different doses of borax. Rats were used as much as 10 animals were divided into 4 groups. The control group (K1) 1 rat, K2 ( borax 20mg), K3 (borax 30 mg), K4 (borax 40 mg) each of 3 rats by administration for 1,5 and 10 days. Result: The result showed the rats had grossly enlarged liver size, liver weight gain and blackish brown in the group given 40 mg of borax and microscopically, the cells were exposed to borax liver showed hydropic degeneration, proliferation fibrolas and fibrosis. From this study it can be concluded that the administraion of borax 20mg, 30mg, 40mg for 10 days caused fatty liver and mild fibrosis

    Pengaruh Pemberian Umbi Bengkuang (Pachyrrhizus Erosus L Urban) Terhadap Jumlah Pigmen Melanin Kulit Mencit (Mus Musculus) Yang Dipaparkan Sinar Matahari

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    : Exposure to sunlight causes melanocytes activity increased so as to increase the production of melanin pigment. Yam (Pachyrrhizus erosus l urban ) contains vitamin C and starch that are opaque can be used to protect the skin from sun exposure. This study aimed to determine the effect of yam tubers to the amount of melanin pigment of skin of mice exposed to sunlight. The method used in this study is an experimental laboratory to the subject of 20 mice were divided into 4 groups. A group is a negative control, B group mice were given exposure to the sun for 20 days. C group mice jicama juice smeared then exposed to sunlight for 20 days, group D mice exposed to sunlight and then stopped after 20 days followed by administration of yam 10 days. The results of group A has an average number of pigment 7, group B 89.5, group C 36, and group D 7,5. In conclusion exposure to sunlight increases the amount of melanin pigment and giving yam decreases the amount of melanin pigment

    Pengaruh Pemberian Air Perasan Jeruk Nipis (Citrus Aurantifolia) Terhadap Jumlah Pigmen Melanin Kulit Mencit (Mus Musculus) Yang Dipaparkan Sinar Matahari

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    : Over exposure of light can increase skin pigmentation. The aim of this study is to know the effect of lime and light exposure to the amount of melanin in mice's skin. This is an experimental research and used mices as the subject research, which was randomly divided into 5 mice of control group and 15 mices of treatment group. Group A (K-) were given pellets for 20 days, Group B (K+) were given pellets and light exposure for 1 hour in 20 days, Group C were given pellets, smeared by lime on the back, then exposed to the light for 1 hour in 20 days, Group D were given pellets and exposed to light for 1 hour in 20 days then smeared by lime the next 10 days. The results showed that light exposure increases melanin pigment in group B compared to group A. For group C and group D, the amount of melanin pigment is less than group B. Conclusions: Lime can reduce the amount of melanin pigment in mice's skin that had been exposed to the light

    Pengaruh Pemberian Ekstrak Biji Kakao (Theobroma Cacao) Terhadap Jumlah Pigmen Melanin Kulit Tikus Wistar (Rattus Novergicus) Yang Dipapar Sinar Matahari

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    : Excessive exposure of sunlight may cause hyperpigmentation. Cocoa is a beneficial plant to the skin. This study was aimed to reveal the effect of cocoa bean extracts on the number of melanin pigments in rat skin after sun exposure. This was an experimental study using 25 Wistar rats (Rattus norvegicus) divided into one control group and four treatment groups. The treatment consisted of sunlight exposure for one hour/day and application of cocoa bean extract 1600 mg/day that varied among treatment groups. Group A was the negative control group (terminated at day 21). Group B, the treatment group 1, was exposed to sunlight for 30 days (terminated at day 31). Group C, the treatment group 2, was exposed to sunlight for 20 days (terminated at day 31). Group D, the treatment group 3, was divided into group D1 consisted of 2 rats and group D2 consisted of 3 rats. Group D1 was exposed to sunlight 30 minutes after the application of cocoa bean extract for 20 days (terminated at day 21). Group D2 was exposed to sunlight 30 minutes after the application of cocoa bean extract for 30 days (terminated at day 31). Group E was exposed to sunlight for 20 days and continued with the application of cocoa bean extract for the next 10 days (terminated at day 3). The results showed that sunlight exposure increased the number of melanin pigments in group B and C compared to group A. Group D showed fewer melanin pigments than group B and C. Group E showed fewer melanin pigments than group B, C, and D. Conclusion: Cocoa bean extract could reduce the number of skin melanin pigments in rats exposed to sunlight
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