59 research outputs found

    Association of serum lipid components and obesity with genetic ancestry in an admixed population of elderly women

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    The prevalence of metabolic disorders varies among ethnic populations and these disorders represent a critical health care issue for elderly women. This study investigated the correlation between genetic ancestry and body composition, metabolic traits and clinical status in a sample of elderly women. Clinical, nutritional and anthropometric data were collected from 176 volunteers. Genetic ancestry was estimated using 23 ancestry-informative markers. Pearsons correlation test was used to examine the relationship between continuous variables and an independent samples t-test was used to compare the means of continuous traits within categorical variables. Overall ancestry was a combination of European (57.49%), Native American (25.78%) and African (16.73%). Significant correlations were found for European ancestry with body mass index (r = 0.165; p = 0.037) and obesity (mean difference (MD) = 5.3%; p = 0.042). African ancestry showed a significant correlation with LDL (r = 0.159, p = 0.035), VLDL (r = -0.185; p = 0.014), hypertriglyceridemia (MD = 6.4%; p = 0.003) and hyperlipidemia (MD = 4.8%; p = 0.026). Amerindian ancestry showed a significant correlation with triglyceride levels (r = 0.150; p = 0.047) and hypertriglyceridemia (MD = 4.5%; p = 0.039). These findings suggest that genetic admixture may influence the etiology of lipid metabolism-related diseases and obesity in elderly women

    Opsoclonus-myoclonus-ataxia syndrome associated with chikungunya and dengue virus co-infection

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-09-19T16:55:58Z No. of bitstreams: 1 Rosario MS Opsoclonus-Myoclonus-Ataxia Syndrome....pdf: 514736 bytes, checksum: 2d645cdbe4121ce8c8d637e1a2c7ed2d (MD5) Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-09-19T17:27:26Z (GMT) No. of bitstreams: 1 Rosario MS Opsoclonus-Myoclonus-Ataxia Syndrome....pdf: 514736 bytes, checksum: 2d645cdbe4121ce8c8d637e1a2c7ed2d (MD5) Made available in DSpace on 2018-09-19T17:27:26Z (GMT). No. of bitstreams: 1 Rosario MS Opsoclonus-Myoclonus-Ataxia Syndrome....pdf: 514736 bytes, checksum: 2d645cdbe4121ce8c8d637e1a2c7ed2d (MD5) Previous issue date: 2018 CNPq-National Council for Scientific and Technological Development (302584/2015-3) and MCTI-Ministry of Science, Technology, Innovation/FINEP‚ÄďFunding Authority for Studies and Projects/FNDCT‚ÄďNational Fund for the Development of Science and Technology (04160060-00/2016) Funda√ß√£o Oswaldo Cruz. Instituto Gon√ßalo Moniz. Salvador, BA, Brasil / Secretaria Estadual da Sa√ļde da Bahia. Hospital Geral Roberto Santos. Salvador, BA, Brasil Funda√ß√£o Oswaldo Cruz. Instituto Gon√ßalo Moniz. Salvador, BA, Brasil / University of Rome Tor Vergata. Rome, Italy Secretaria Estadual da Sa√ļde da Bahia. Hospital Geral Roberto Santos. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil Secretaria Estadual da Sa√ļde da Bahia. Hospital Geral Roberto Santos. Salvador, BA, Brasil University of Oxford. Department of Zoology. Oxford, UK Instituto Evandro Chagas. Centro de Tecnologia e Inova√ß√£o. Ananindeua, PA, Brasil Instituto Evandro Chagas. Centro de Tecnologia e Inova√ß√£o. Ananindeua, PA, Brasil Instituto Evandro Chagas. Centro de Tecnologia e Inova√ß√£o. Ananindeua, PA, Brasil Funda√ß√£o Oswaldo Cruz. Instituto Gon√ßalo Moniz. Salvador, BA, Brasil Funda√ß√£o Oswaldo Cruz. Instituto Gon√ßalo Moniz. Salvador, BA, Brasil Opsoclonus-myoclonus-ataxia syndrome (OMAS) is a rare neurological disorder characterized by irregular multidirectional eye movements, myoclonus, cerebellar ataxia, sleep disturbances, and cognitive dysfunction. Although most commonly related to paraneoplastic syndrome, this condition has occasionally been described following infectious illnesses. This article reports the first case of OMAS in association with chikungunya and dengue virus co-infection. The genetic analysis identified chikungunya virus of East/Central/South African genotype and dengue serotype 4 virus of genotype II. This report represents an unusual clinical syndrome associated with viral co-infection and reinforces the need for clinical vigilance with regard to neurological syndromes in the context of emergent arboviruses

    Assessment of cytotoxicity in three tumor cell lines of the fractions attained from the stem bark of salacia crassifolia (mart. ex. schult.) g. dom (celastraceae)

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    For millennia humans have been using plants for medicinal purposes to treat, cure, or prevent diseases, including cancer. Brazilian ecosystem has a high diversity of plant species. From this perspective, a species found in the Cerrado,¬†Salacia crassifolia¬†(Celastraceae), was selected, for an initial screening to determine the cytotoxic potential of this species "in vitro".¬†S. crassifolia¬†has been widely used in traditional medicine to treat kidney diseases, chronic cough, headaches, healing, ulcerogenic, and for treatment of malaria. The fractions: hydroalcoholic (SCCcM-W), dichloromethane(SCCcM-D), hexane (SCCcM-H) and ethyl acetate (SCCcM-A) from the stem bark were tested for cytotoxicity "in vitro" to within MDA -MB-435(melanoma), HCT-8 (human-colon), and SF-295 (CNS) using the MTT colorimetric method. The hexane (SCCcM-H) and ethyl acetate (SCCcM-A) fractions were those that provided significant cytotoxicity against analyzed tumor cells.A utiliza√ß√£o de plantas pela humanidade com fins medicinais √© uma das formas mais antigas para tratamento, cura e preven√ß√£o de doen√ßas, dentre elas o c√Ęncer, cujas esp√©cies vegetais s√£o encontradas em elevada diversidade no ecossistema. Com essa perspectiva, se optou por um arbusto t√≠pico do Cerrado,¬†Salacia crassifolia¬†(Celastraceae), para um¬†screening¬†inicial na determina√ß√£o do potencial citot√≥xico desta esp√©cie¬†in vitro. Na medicina popular,¬†S. crassifolia√© utilizada no tratamento de problemas renais, tosse cr√īnica, dores de cabe√ßa, cicatrizantes, ulcerog√™nicas e na terapia da malaria. As fra√ß√Ķes: hidroalco√≥lica (SCCcM-W), dicloromet√Ęnica(SCCcM-D), hex√Ęnica (SCCcM-H) e acetato de etila (SCCcM-A) da casca do caule foram submetidas a testes de citotoxicidade¬†in vitro¬†frente √†s linhagens MDA-MB-435(melanoma), HCT-8 (c√≥lon-humano) e SF-295 (sistema nervoso central) utilizando o m√©todo colorim√©trico MTT. As fra√ß√Ķes hex√Ęnica (SCCcM-H) e de acetato de etila(SCCcM-A) foram aquelas que proporcionaram citotoxicidade significativas frente √†s c√©lulas tumorais analisadas

    Insights Into Limnothrix sp. Metabolism Based on Comparative Genomics

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    Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism

    Growth or somatotrophic hormone: new perspectives in isolated GH deficiency after description of the mutation in the GHRH receptor gene in individuals of Itabaianinha county, Brazil

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    Al√©m de influenciar o crescimento corp√≥reo, o horm√īnio do crescimento, ou somatotr√≥fico, desempenha importante papel no metabolismo, composi√ß√£o corporal, perfil lip√≠dico, estado cardiovascular e longevidade. Seu controle √© multi-regulado por horm√īnios, metab√≥litos e pept√≠deos hipotal√Ęmicos. Dados sobre a Defici√™ncia Isolada de GH (DIGH) obtidos a partir da descri√ß√£o da muta√ß√£o IVS1+1G‚ÜíA no gene do receptor do horm√īnio liberador do GH (GHRH-R) em indiv√≠duos da cidade de Itabaianinha, SE, s√£o revisados. S√£o abordadas novas perspectivas sobre o modelo de resist√™ncia ao GHRH, a import√Ęncia do GHRH no controle da secre√ß√£o de GH, a freq√ľ√™ncia das muta√ß√Ķes do gene do GHRH-R, a relev√Ęncia diagn√≥stica do IGF-I e os achados metab√≥licos, cardiovasculares e de qualidade de vida nestes indiv√≠duos._________________________________________________________________________________________ ABSTRACT: In addition to stimulating body growth, growth or somatotrophic hormone plays an important role in metabolism, body composition, lipid profile, cardiovascular status and longevity. Its control is multiregulated by hormones, metabolites and hypothalamic peptides. Obtained data of the isolated growth hormone deficiency (IGHD) after the description of the IVS1+1G‚ÜíA GHRH receptor gene mutation in individuals of Itabaianinha County are reviewed. New perspectives about the growth hormone resistance model, the importance of GHRH in the control of GH secretion, the frequency of GHRH-R gene mutations, the diagnostic relevance of IGF-I and the metabolic, cardiovascular and quality of life findings are approached

    Human pegivirus (HPgV, GBV-C) RNA in volunteer blood donors from a public hemotherapy service in Northern Brazil

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    HEMOPA foundation, Evandro Chagas Institute, Foundation for Scientific and Technological Development in Health (FIOTEC; Project PRES-012-FIO-16) and funded in part by the research productivity project CNPq (302584/2015‚Äď3)Par√° State University. Center for Life Science and Health. Bel√©m, PA, Brazil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Par√° State University. Center for Life Science and Health. Bel√©m, PA, Brazil.Foundation Center for Hemotherapy and Hematology of Par√°. Bel√©m, PA, Brazil.Foundation Center for Hemotherapy and Hematology of Par√°. Bel√©m, PA, Brazil.Ophir Loyola Hospital. Bel√©m, PA, Brazil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Par√° State University. Center for Life Science and Health. Bel√©m, PA, Brazil.Human pegivirus (HPgV)‚ÄĒformerly known as GBV-C‚ÄĒis a member of the Flaviviridae family and belongs to the species Pegivirus C. It is a non-pathogenic virus and is transmitted among humans mainly through the exposure to contaminated blood and is often associated with human immunodeficiency virus (HIV) infection, among other viruses. This study aimed to determine the prevalence of HPgV viremia, its association with HIV and clinical epidemiological factors, as well as the full-length sequencing and genome characterization of HPgV recovered from blood donors of the HEMOPA Foundation in Bel√©m-PA-Brazil. Methods: Plasma samples were obtained from 459 donors, tested for the presence of HPgV RNA by the RT-qPCR. From these, a total of 26 RT-qPCR positive samples were submitted to the NGS sequencing approach in order to obtain the full genome. Genome characterization and phylogenetic analysis were conducted. Results: The prevalence of HPgV was 12.42%. We observed the highest prevalences among donors aged between 18 and 30 years old (16.5%), with brown skin color (13.2%) and men (15.8%). The newly diagnosed HIV-1 prevalence was 26.67%. The HPgV genotype 2 (2a and 2b) was identified. No data on viral load value was found to corroborate the protective effect of HPgV on HIV evolution. Conclusions: This study provided information regarding the HPgV infection among blood donors from HEMOPA Foundation. Furthermore, we genetically characterized the HPgV circulating strains and described by the first time nearly complete genomes of genotype 2 in Brazilian Amazon

    New virus genome sequences of the Guama Serogroup (Genus Orthobunyavirus, Family Bunyaviridae), isolated in the Brazilian Amazon Region

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    Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.This is the first announcement of two nearly complete viral genome sequences belonging to the Guama serogroup (genus Orthobunyavirus, family Bunyaviridae) isolated in the Brazilian Amazon region: Mirim virus (MIRV; BEAN7722) and Ananindeua virus (ANUV; BEAN109303)

    Analysis of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) for yellow fever diagnostic

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    This study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Cient√≠fico e Tecnol√≥gico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0).Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Centro de Inova√ß√Ķes Tecnol√≥gicas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Minist√©rio da Sa√ļde. Secretaria de Vigil√Ęncia em Sa√ļde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Para State. Department of Pathology. Belem, PA, Brazil.University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA.Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas
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