7 research outputs found


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    Additional file 1: Table S1. The chromosome, position, reference and alternative bases for the 193 SNVs and 17 indels that were verified as being true somatic calls are listed here. The variant read frequency observed in the verification sequencing is also listed here

    Frequency of STR marker detection.

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    <p>T1-2/N1-2, T2-2/N2-2, T3S1-2/T3S2-2/N3-2, T4-2/N4-2 tumor and normal samples are represented by bars only when an STR marker was successfully amplified. Nucleotide size ranges are listed above the corresponding STR marker.</p

    SNP amplicon size does not indicate SNP call failure.

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    <p>All 45–139 bp. One SNP amplicon of 128 bp (dbSNP ID rs733164) does not amplify in all instances. For R1 132, amplification fails for 14 additional SNPs ranging in size from 97–139 bp.</p

    FFPE tumor/normal pair relatedness assessed by Sequenom SNP genotyping.

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    <p>Heat map analysis plots generated by R v2.15.1 visualize the number of matched SNP calls between paired samples. Positive (Pos1-T/N and Pos2-T/N) and negative (Neg1 and Neg2) controls are included in each heat map. Match failures are considered to be in the range of 0–38 SNP matches, while matched samples (38–45 SNP matches) are shown in pink.</p

    Development and Manufacture of a Curtius Rearrangement Using Continuous Flow towards the Large-Scale Manufacture of AZD7648

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    Flow chemistry has been more frequently used in the development and manufacture of pharmaceuticals due to the progression of equipment and the availability of manufacturing facilities. This important tool for manufacture enables chemistries which were previously inefficient, hazardous, or not viable on scale in batch. Described herein is how continuous flow has been used to overcome issues with respect to the handling of hazardous materials in batch. The manufacturing step described is a Curtius reaction using diphenylphosphoryl azide (DPPA) as the azide precursor in the formation of a purinone at high temperatures with inline infrared monitoring