Resampling and requantization of band-limited Gaussian stochastic signals with flat power spectrum

A theoretical analysis, aimed at characterizing the degradation induced by the resampling and requantization processes applied to band-limited Gaussian signals with flat power spectrum, available through their digitized samples, is presented. The analysis provides an efficient algorithm for computing the complete {joint} bivariate discrete probability distribution associated to the true quantized version of the Gaussian signal and to the quantity estimated after resampling and requantization of the input digitized sequence. The use of Fourier transform techniques allows deriving {approximate} analytical expressions for the quantities of interest, as well as implementing their efficient computation. Numerical experiments are found to be in good agreement with the theoretical results, and confirm the validity of the whole approach.Comment: Submitted to Digital Signal Processin

Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue

A one-step enzymatic reaction for improving the collision-induced dissociation (CID)-based tandem mass spectrometry (MS/MS) analysis of phosphorylated peptides in an ion trap is presented. Carboxypeptidase-B (CBP-B) was used to selectively remove C-terminal arginine or lysine residues from phosphorylated tryptic/Lys-C peptides prior to their MS/MS analysis by CID with a Paul-type ion trap. Removal of this basic C-terminal residue served to limit the extent of gas-phase neutral loss of phosphoric acid (H(3)PO(4)), favoring the formation of diagnostic b and y ions as determined by an increase in both the number and relative intensities of the sequence-specific product ions. Such differential fragmentation is particularly valuable when the H(3)PO(4) elimination is so predominant that localizing the phosphorylation site on the peptide sequence is hindered. Improvement in the quality of tandem mass spectral data generated by CID upon CBP-B treatment resulted in greater confidence both in assignment of the phosphopeptide primary sequence and for pinpointing the site of phosphorylation. Higher Mascot ion scores were also generated, combined with lower expectation values and higher delta scores for improved confidence in site assignment; Ascore values also improved. These results are rationalized in accordance with the accepted mechanisms for the elimination of H(3)PO(4) upon low energy CID and insights into the factors dictating the observed dissociation pathways are presented. We anticipate this approach will be of utility in the MS analysis of phosphorylated peptides, especially when alternative electron-driven fragmentation techniques are not available. [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13361-013-0770-2) contains supplementary material, which is available to authorized users

Coupling radio propagation and weather forecast models to maximize Ka-band channel transmission rate for interplanetary missions

Deep space (DS) missions for interplanetary explorations are aimed at acquiring information about the solar system and its composition. To achieve this result a radio link is established between the space satellite and receiving stations on the Earth. Significant channel capacity must be guaranteed to such spacecraft-to-Earth link considering their large separation distance as well. Terrestrial atmospheric impairments on the space-to-Earth propagating signals are the major responsible for the signal degradation thus reducing the link’s channel temporal availability. Considering the saturation and the limited bandwidth of the conventional systems used working at X-band (around 8.4 GHz), frequencies above Ku-band (12-18 GHz) are being used and currently explored for next future DS missions. For example, the ESA mission EUCLID, planned to be launched in 2020 to reach Sun-Earth Lagrange point L2, will use the K-band (at 25.5-27 GHz). The BepiColombo (BC) ESA mission to Mercury, planned to be launched in 2016, will use Ka-band (at 32-34 GHz) with some modules operating at X-band too. The W-band is also being investigated for space communications (Lucente et al., IEEE Systems J., 2008) as well as near-infrared band for DS links (Luini at al., 3rd IWOW, 2014; Cesarone et al., ICSOS, 2011). If compared with X-band channels, K-band and Ka-band can provide an appealing data rate and signal-to-noise ratio in free space due to the squared-frequency law increase of antenna directivity within the downlink budget (for the same physical antenna size). However, atmospheric path attenuation can be significant for higher frequencies since the major source of transmission outage is not only caused by convective rainfall, as it happens for lower frequencies too, but even non-precipitating clouds and moderate precipitation produced by stratiform rain events are detrimental. This means that accurate channel models are necessary for DS mission data link design at K and Ka band. A physical approach can offer advanced radiopropagation models to take into account the effects due to atmospheric gases, clouds and precipitation. The objective of this work is to couple a weather forecast numerical model with a microphysically- oriented radiopropagation model, providing a description of the atmospheric state and of its effects on a DS downlink. This work is developed in the framework of the RadioMeteorological Operations Planner (RMOP) program, aimed at performing a feasibility study for the BC mission (Biscarini et al., EuCAP 2014). The RMOP chain for the link budget computation is composed by three modules: weather forecast (WFM), radio propagation (RPM) and downlink budget (DBM). WFM is aimed at providing an atmospheric state vector. Among the available weather forecast models, for RMOP purposes we have used the Mesoscale Model 5. The output of the WFM is the input of the RPM for the computation of the atmospheric attenuation and sky-noise temperature at the receiving ground station antenna. RPM makes use of radiative transfer solver based on the Eddington approximations well as accurate scattering models. Time series of attenuation and sky-noise temperature coming from the RPM are converted into probability density functions and then ingested by the DBM to compute the received data volume (DV). Using the BC mission as a reference test case for the Ka-band ground station at Cebreros (Spain), this work will show the advantages of using a coupled WFM-RPM approach with respect to climatological statistics in a link budget optimization procedure. The signal degradation due to atmospheric effects in DS links in terms of received DV will be also investigated not only at Ka band, but also at X, K and W for intercomparison. The quality of the DS downlink will be given in terms of received DV and the results at different frequencies compared showing the respective advantages and drawbacks

The Northern Adriatic Forecasting System for Circulation and Biogeochemistry: Implementation and Preliminary Results

This paper described the implementation of a forecasting system of the coupled physical and biogeochemical state of the northern Adriatic Sea and discussed the preliminary results. The forecasting system is composed of two components: the NEMO general circulation model and the BFM biogeochemical model. The BFM component includes an explicit benthic pelagic coupling providing fluxes at the sediment-water interface and the dynamic of the major benthic state variables. The system is forced by atmospheric forcing from a limited-area model and by available land-based (river runoff and nutrient load) data. The preliminary results were validated against available remote and in situ observations. The validation effort indicated a good performance of the system in defining the basin scale characteristics, while locally the forecasting model performance seemed mostly impaired by the uncertainties in the definition of the land-based forcing

Flow regimes study within the Strait of Gibraltar using a high-performance numerical model

A three-dimensional sigma coordinate free-surface high-performance model is used to investigate the flow regimes within the Strait of Gibraltar. High performances are achieved through a directive-based, MPI-like, parallelization of the code, obtained using SMS tool. The model makes use of a coastal-following, curvilinear orthogonal grid, that includes the Gulf of Cadiz and the Alboran Sea, reaching very high resolution in the Strait. Four experiments with different initial salinity conditions representing the present and possible future climate conditions over the Mediterranean basin have been performed. Model results, analysed by means of the three-layer composite Froude number theory, have shown two different possible regimes within the strait; for the present climate condition the strait is subjected to a sub-maximal regimewhilefor possible future climate conditions a maximal regime can be reached

Climate variations in the Northern Hemisphere based on the use of an atmosphere-ocean IPCC model

Forced and natural variability of modelled and observed Atlantic Ocean temperature and Atlantic Meridional Overturning Circulation (AMOC) is studied. In the observations and in a forced climate model run, we find increasing temperature at 1000m in the Atlantic (20N). SVD analysis shows that, for both model data and observations, a high index of North Atlantic Oscillation (NAO) corresponds to negative temperature anomaly at 1000m to the north of 55N, although geographical details of temperature anomaly distribution are different for the model and observations. Particular attention has been paid to the influence of the fresh water flux due to the present global warning on the slowing down of the AMOC. It is shown that fresh water flux change is only a secondary cause of reduced AMOC in global warming conditions, while heat flux change is probably the main reason. Finally, it is shown that internal model AMOC variability is positively correlated with the near-surface air temperature in Atlantic-European Arctic sector on a 10-year time scale