91 research outputs found

    Magnetic Self-Assembling of spherical Co nanoparticles used as building blocks: Syntheses, properties and theory

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    International audienceIn this chapter, we show that thanks to the use of micellar and organometallic approaches, one can favor the growth of uniform spherical Co NPs with controlled surface passivation (dodecanoic acid or oleylamine), tunable size (from around 4 to 9 nm) and tunable nanocrystallinity (from fcc to hcp structure). As a result of the balance between van der Waals attractions between the metallic NPs, magnetic interactions between the magnetic NPs and solvent-mediated interactions between ligands, these uniform colloidal NPs can be used as building units to form a full set of assemblies which morphology depends on the deposition strategy, involving solvent evaporation. In the case of spontaneous self-assembling of magnetic NPs, compact hexagonal 2D arrays and 3D superlattices called supercrystals can form. In the latter case, either face-centered cubic supercrystalline films or single colloidal crystals can be obtained. Mesostructures of hexagonally ordered columns, labyrinths and void structures can result from assisted self-assembling, induced by the application of an external magnetic field. In highly ordered superlattices, individual NPs act as “artificial atoms” and occupy the lattice sites to form repetitive, periodic “artificial planes". From a fundamental point of view, these artificial solids constitute good models for investigating crystallization behavior. Resulting from collective interactions between neighboring NPs, they exhibit novel magnetic properties. The magnitude of these interactions, and then, the magnetic properties, can be tuned by various parameters including (1) the (crystallographic) nature of the magnetic NP, (2) the NP size, (3) the nature of the coating agent, (4) the nature of the solvent, (5) the evaporation rate and (6) if appropriate, the application of an external field during the solvent evaporation. On the one hand, simulations based on a flory-type solvation theory using Hansen solubility colloidal parameters allow to predict the cobalt NP size. On the other hand, Monte Carlo simulations and free energy theories are able to predict the size and type of patterns appearing during the evaporation of a solution of magnetic NPs under a magnetic fiel

    Dextrin-based nanomagnetogel: in vivo biodistribution and stability

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    The biodistribution profile of a new dextrin nanomagnetogel, which consists on -Fe2O3 superparamagnetic nanoparticles loaded within a polymeric matrix of modified dextrin, was studied in mice. The nanomagnetogel bear a monomodal size distribution profile (average diameter 110 nm) close to neutral surface charge and higher relaxivity (r2 = 215-248 mM-1s-1 and r2/r1 = 13-11) than those of commercial formulations (r2 = 160-177 mM-1s-1 and r2/r1 = 4-7). Also the observed blood half-life - approximately 4 hours - is superior to that of similar commercially available formulations, which remain few minutes in circulation. Pegylation resulted in 1.7 and 1.2-fold lower accumulation in the liver and spleen, respectively, within the first 24 h. Noteworthy, a good correlation was obtained between the amount of polymer (quantified by scintigraphy) in the spleen, 48 h after administration, and the amount of iron physically loaded through hydrophobic interactions (quantified by ICP) indicating the absence of iron leakage from the polymeric matrix. This study provides evidence on the in vivo stability of a self-assembled nanomagnetogel, a much relevant feature which is seldom reported in the literature.The authors thank the Project “strong>BioHealth - Biotechnology and Bioengineering approaches to improve health quality”, Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 O Novo Norte), QREN, FEDER. The authors thank the Magnisense Corporation for providing a MIAplex Reader and CFGCG the EU COST TD1004 Action “Theragnostics Imaging and Therapy”. The authors thank Professor Cidália Botelho for the iron analysis by Atomic Absorbance Spectroscopy at the Oporto University, Chemical Engineering Department. C. Gonçalves, J. P. Silva, J. A. Martins, and M. F. M. Ferreira acknowledge FCT Portugal, for postdoc grants SFRH/BPD/70524/2010 and SFRH/BPD/64958/2009, sabbatical grant SFRH/BSAB/ 1328/2013 and PhD grant SFRH/BD/63994/2009, respectively

    Viologen-Templated Arrays of Cucurbit[7]uril-Modified Iron-Oxide Nanoparticles

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    International audienceMagnetic and fluorescent assemblies of iron-oxide nanoparticles (NPs) were constructed by threading a viologen-based ditopic ligand, DPV2+, into the cavity of cucurbituril (CB[7]) macrocycles adsorbed on the surface of the NPs. Evidence for the formation of 1:2 inclusion complexes that involve DPV2+ and two CB[7] macrocycles was first obtained in solution by 1H NMR and emission spectroscopy. DPV2+ was found to induce self-assembly of nanoparticle arrays (DPV2+⊂CB[7]NPs) by bridging CB[7] molecules on different NPs. The resulting viologen-crosslinked iron-oxide nanoparticles exhibited increased saturation magnetization and emission properties. This facile supramolecular approach to NP self-assembly provides a platform for the synthesis of smart and innovative materials that can achieve a high degree of functionality and complexity and that are needed for a wide range of applications

    SiO2 versus chelating agent@ iron oxide nanoparticles: interactions effect in nanoparticles assemblies at low magnetic field

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    International audienceHydrophilic magnetic nanoparticles present many interest for various medical applications due to their unique properties: immunoassays, imaging and hyperthermia. With regards to their applicability in the biomedical field, colloidal stability is a key parameter related to nanoparticle surface functionalization. In this paper, we report the water transfer of hydrophobic oleic acid coated iron oxide nanoparticles comparing two methodologies to obtain water dispersible iron oxide nanoparticles: exchange ligands with small strong chelating agent (caffeic acid) and SiO2 shell passivation. Both strategies are leading to stable aqueous ferrofluid but differing by their interactions. The non linear magnetic behavior at high and low magnetic field and second derivative signature of water dispersed superparamagnetic Fe304 nanoparticles samples are studied using conventional SQUID equipment and miniaturized detector MIAplexÂź device. We demonstrated those samples differing only by their interparticle interactions present different magnetic behavior at very low magnetic field whereas at high magnetic field both samples are very similar

    Structural mechanism underlying capsaicin binding and activation of the TRPV1 ion channel.

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    Capsaicin bestows spiciness by activating TRPV1 channel with exquisite potency and selectivity. Although a capsaicin-bound channel structure was previously resolved by cryo-EM at 4.2- to 4.5-Å resolution, capsaicin was registered as a small electron density, reflecting neither its chemical structure nor specific ligand-channel interactions--important details required for mechanistic understanding. We obtained the missing atomic-level details by iterative computation and confirmed them by systematic site-specific functional tests. We observed that the bound capsaicin takes a 'tail-up, head-down' configuration. The vanillyl and amide groups form specific interactions to anchor its bound position, while the aliphatic tail may sample a range of conformations, making it invisible in cryo-EM images. Capsaicin stabilizes TRPV1's open state by 'pull-and-contact' interactions between the vanillyl group and the S4-S5 linker. Our study provides a structural mechanism for the agonistic function of capsaicin and its analogs, and demonstrates an effective approach to obtain atomic-level information from cryo-EM structures