13 research outputs found

    Direct Determination of Heavy Metals in Honey by Potentiometric Stripping Analysis

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    Honey is a valuable nutritious food rich in simple sugars, aminoacids, proteins and minerals. In addition, this food product contains natural phenols and flavonoids, responsible for some bioactive properties. Due to known health benefits of honey and its exquisite flavour this foodstuff is readily consumed by children and wide population imposing necessity of strict quality control. One of quality criteria is the content of heavy metals. Elevated content of heavy metals in honey may result from environmental or processing contamination, or may originate from soil or plant species that honey is derived from.In this work twenty five samples of honey from Serbia, Montenegro and Bosnia and Herzegovina were analysed in respect to zinc, cadmium, lead and copper content. Honey samples were analysed directly, without sample preparation, by applying potentiometric stripping analysis. Zinc, cadmium and lead were quantitated by using indium as an internal standard, whereas copper was determined by standard addition method. Method accuracy was confirmed by blind analysis of standard samples and with good recovery results which, for analysed samples, were in the range 91-101%. In all analysed sampled determined metals contents were in the range permitted by Serbian regulations. Cadmium was not detected in any of the analysed samples, whereas the content of other metals were in the range of 0.01-3.6 mg/g for zinc, 0.02-0.8 mg/g for lead and 0.1-2.4 mg/g for copper, respectively

    Polyphenolic Profile of Sambucus Ebulus Root, Leaf and Fruit Extracts

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    Sambucus ebulus L. is a perennial herbaceous plant popular in folk medicine in Western Europe, Balkan and Middle East regions. Its preparations and extracts have shown wide range of biological activities against various disease and conditions. Isolation of the phenolic components was conducted using microwave-assisted extraction technique, while identification and quantification of isolated compounds were conducted using HPLC-DAD analysis. Fourteen compounds were detected and quantified in the extracts of the plant root, leaves and fruit, whereby rutin was predominant compound in all three samples. Minor compound in root sample was ferulic acid, in the leaves extract was chlorogenic acid, while in fruit sample was luteolin. Contrary, protocatehuic acid, caffeic acid, luteolin glycoside and apigenin glycoside were not detected in any of analyzed extracts

    Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum offcinale L.) Root Extracts Obtained by Different Extraction Techniques

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    In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of S. offcinale root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more effcient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 ºC. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.This work was funded by the project financially supported by the Serbian Ministry of Education, Science and Technological Development (TR 31014), the Andalusian Regional Government Council of Innovation and Science (P11-CTS-7625), the Spanish Ministry of Economy and Competitiveness (MINECO) (AGL2015-67995-C3-2, IJCI-2015-26789, and PTQ-13-06429), and the European Social Fund (FSE) (PTQ-13-06429)

    Nutritional Insights and Food Safety

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    vii. 426 hal.. 25.5 c

    Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum offcinale L.) Root Extracts Obtained by Different Extraction Techniques

    No full text
    In this work a comparative study on phytochemical profiles of comfrey root extracts obtained by different extraction approaches has been carried out. Chemical profiles of extracts obtained by supercritical fluid (SFE), pressurized liquid (PLE), and conventional solid/liquid extraction were compared and discussed. Phytochemical composition was assessed by high-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) identifying 39 compounds reported for the first time in comfrey root, mainly phenolic acids and fatty acids. The influence of different extraction parameters on phytochemical profiles of S. offcinale root was investigated for all applied techniques. PLE and maceration, using alcohol-based solvents (aqueous methanol or ethanol), were shown to be more effcient in the recovery of more polar compounds. Greater numbers of phenolics were best extracted by PLE using 85% EtOH at 63 ºC. The use of SFE and 100% acetone for 30 min enabled good recoveries of nonpolar compounds. SFE using 15% EtOH as a cosolvent at 150 bar produced the best recoveries of a significant number of fatty acids. The main compositional differences between extracts obtained by different extraction techniques were assigned to the solvent type. Hence, these results provided comprehensive approaches for treating comfrey root enriched in different phytochemicals, thereby enhancing its bioaccessibility.This work was funded by the project financially supported by the Serbian Ministry of Education, Science and Technological Development (TR 31014), the Andalusian Regional Government Council of Innovation and Science (P11-CTS-7625), the Spanish Ministry of Economy and Competitiveness (MINECO) (AGL2015-67995-C3-2, IJCI-2015-26789, and PTQ-13-06429), and the European Social Fund (FSE) (PTQ-13-06429)
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