8 research outputs found

    DR2539 binds to the MntH promoter DNA fragment in a Mn(II)- and Fe(II)-dependent manner.

    No full text
    <p>(A) Schematic of <i>dr1709</i> promoter (p1709a and p1709b) DNA sequence region. The inverted repeat region is shown by the inverted arrows. (B) DR2539 binding to p1709b with increasing quantities of DR2539 and 25 ┬ÁM Mn(II). (C) and (D) EMSA analysis was performed using DR2539 and p1709b with increasing concentration of Mn(II) or Fe(II). RBS, ribosome binding site; Start, transcription start codon. p1709a and p1709b sequence regions are underlined by straight lines and dashed lines, respectively.</p

    Mn(II) and Fe(II) modulate the binding activity of DR2539 <i>in vivo</i>.

    No full text
    <p>(A) Effects of divalent metals (50 ┬ÁM) on expression of pRAZH in <i>D. radiodurans</i>. Data shown are the means ┬▒ standard deviations of three independent experiments. (B) Effects of Mn(II) (squares) and Fe(II) (circles) on the expression of pRAZH in wild-type samples expressing DR2539. (C) Effects of Mn(II) (squares) and Fe(II) (circles) on the expression of pRAZH in the <i>dr2539</i> null mutant. (D) Rea-time PCR analysis of the <i>dr1709</i> gene expression using <i>dr0089</i> as internal control gene. Longitudinal axes indicate the change fold of <i>dr1709</i> mRNA relative to controls. Control cells were cultured in medium without Mn(II). *, <i>P</i><0.05 relative to control. The data are the means ┬▒ standard deviations of three independent experiments.</p

    Sequence alignment of the metal binding sites of DR2539 with other DxtR/MntR family members.

    No full text
    <p>ScaR (<i>Streptococcus gordonii</i>), SloR (<i>Streptococcus suis</i>), LCAS (<i>Lactobacillus casei</i>), SirR (<i>Corynebacterium glutamicum</i>), TroR (<i>Treponema pallidum</i>), TroR (<i>Treponema denticola</i>), IdeR (<i>Mycobacterium tuberculosis</i>), DtxR (<i>Corynebacterium diptheriae</i>), DR2539 (<i>Deinococcus radiodurans</i>), MntR (<i>Staphylococcus aureus</i>), MntR (<i>Escherichia coli</i>), and MntR (<i>Bacillus subtilis</i>). The sequences were aligned using the CLUSTAL W software. Residues shaded with black represent metal-binding sites that have been studied while residues shaded with grey represent predicted metal binding sites.</p

    DR0865 binds to the promoter of MntABC in an ion-dependent manner.

    No full text
    <p>(A) and (B) DR0865 binding to p2523 and p2284 as the concentration of DR0865 increased. (C) Wild-type R1, <i>dr2539</i> null mutant (<b>Δ</b><i>dr2539</i>), and <i>dr0865</i> null mutant (<b>Δ</b><i>dr0865</i>) were cultured on TGY plates overlaid with filter discs saturated with 1 M solution MnCl<sub>2</sub>. (D) The zone of inhibition was measured from edge of disc after three days. *, <i>P</i><0.05. Data represent the means±deviations of three independent experiments.</p

    His98 plays an important role in DNA binding activity of DR2539.

    No full text
    <p>(A) 10 ┬Ál cell dilution was dripped on the TGY plate to which 6 mM of Mn(II) had been added. The cells were cultured for 3 days. (B) H98Y mutant and wild-type DR2539 proteins were incubated with p1709b at different concentrations of Mn(II). (C) Quantification of the fluorescence intensity of binding bands was performed using ImageJ. *, <i>P</i><0.05.</p

    H<sub>2</sub>O<sub>2</sub> sensitivity in <i>D. radiodurans</i> treated with dAMP, dTMP, dCMP or dGMP.

    No full text
    <p>Sensitivity of <i>D. radiodurans</i> to 50 mM H<sub>2</sub>O<sub>2</sub> with the addition of DNA fragments (3.6 mg/ml), dNMPs, dAMP, dTMP, dCMP or dGMP (10 mM for each). Each data point represents the mean┬▒SD of three replicates. R1, <i>D. radiodurans</i> wild type strain.</p

    Addition of extracellular dGMP increases the activity of KatA in <i>D. radiodurans</i>.

    No full text
    <p>(A) Extracellular dGMP (2.5 mM) increased the activity of KatA, but not KatB. (B) Extracellular dGMP (2.5 mM) had no effect on the activity of SOD. (C) and (D) Quantification of the intensity of bands was performed using ImageJ. Each sample contains 80 ┬Ág of total protein. Values are the mean ┬▒ standard deviation of three independent measurements. R1, <i>D. radiodurans</i> wild type strain; KatA, catalase A; KatB, catalase B.</p

    H<sub>2</sub>O<sub>2</sub> sensitivity in <i>D. radiodurans</i> and <i>E. coli</i> treated with DNA fragments or dNMPs.

    No full text
    <p>(A) Sensitivity of <i>D. radiodurans</i> to H<sub>2</sub>O<sub>2</sub> after the addition of 3.6 mg/ml DNA fragments or dNMPs. (B) Sensitivity of <i>E. coli</i> K-12 to H<sub>2</sub>O<sub>2</sub> after the addition of 3.6 mg/ml DNA fragments or dNMPs. Each data point represents the mean┬▒SD of three replicates. R1, <i>D. radiodurans</i> wild type strain.</p
    corecore