41 research outputs found

    Utilization Rate of Outsourcing in Selected Municipalities

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    Cílem bakalářské práce je míra využití outsourcingu v obcích Olomouckého kraje. Zvolený problém jsem vyřešila pomocí metodiky dotazníkového šetření a analýzy získaných dat. Podařilo se získat dostatečné množství vzorků a tím potvrdit, že obce outsourcing využívají. Hlavním přínosem této práce je zjištění, že obce outsourcing využívají ve velké míře. Většina obcí, které odpověděly na dotazník, své činnosti zajišťují externím dodavatelem. Z práce je taky zřejmé, že nejčastější outsourcované činnosti jsou informačních technologie, komunální odpad a právní služby. Obce k zavedení outsourcingu vedou různé důvody, nejčastějším důvodem je přístup k technologiím a lidským zdrojům externím dodavatele. U každého smluvního vztahu mohou vzniknout rizika. Dalším zjištěním je, že nejčastějším rizikem je kvalita poskytované služby a kvalifikace pracovníků dodavatelské firmy.The aim of this thesis is ulitization rate of outsourcing in the selected municipalities of the Olomouc region. The chosen problem I solved using the methodology of the questionnaire survey and the analysis of the obtained data. It was managed to get a sufficient number of samples and thus to confirm that the municipalities use outsourcing. The main contribution of this thesis is findig out that the level of outsourcing use in selected municipalities is very often. The major part of municipalities, which took part in questionnaire survey, make use of external suppliers for their activities. According to questionnaire survey results the most common outsourced activities are information technology, municipal waste and legal services. The municipalities use outsourcing because of a variety of reasons, the most common reason is access to the technology and human resources of external contractor. For each contractual relationship risks can arise. Another finding is that the most common risk is the quality of the provided services and the qualifications of the contractor's staff.153 - Katedra veřejné ekonomikyvýborn

    Plasma concentrations of Lys, Met, Phe, Pro, Ser, Tau, and Thr in response to single-dose duodenal infusions of glucose (DIG), leucine (DIL), or saline (SAL) in dairy cows.

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    <p>Plasma concentrations of Lys, Met, Phe, Pro, Ser, Tau, and Thr in response to single-dose duodenal infusions of glucose (DIG), leucine (DIL), or saline (SAL) in dairy cows.</p

    Significant plasma metabolites (mean ± SE) identified by t-test at 50 and 120 min after duodenal bolus infusions of glucose (DIG) as compared to saline (SAL) in dairy cows.

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    <p>Significant plasma metabolites (mean ± SE) identified by t-test at 50 and 120 min after duodenal bolus infusions of glucose (DIG) as compared to saline (SAL) in dairy cows.</p

    Characteristics of the primers and the real-time PCR conditions.

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    <p><sup>1</sup> Adiponectin, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Lemor1" target="_blank">[45]</a>.</p><p><sup>2</sup> Adiponectin receptor 1, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Lemor1" target="_blank">[45]</a>.</p><p><sup>3</sup> Adiponectin receptor 2, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Lemor1" target="_blank">[45]</a>.</p><p><sup>4</sup> Leptin, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Yuen1" target="_blank">[93]</a>.</p><p><sup>5</sup> Leptin receptor isoform b, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Lemor1" target="_blank">[45]</a>.</p><p><sup>6</sup> Leptin receptor.</p><p><sup>7</sup> Peroxisome proliferator-activated receptor <i>gamma2.</i></p><p><sup>8</sup> Peroxisome proliferator-activated receptor <i>gamma</i>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086211#pone.0086211-Hosseini1" target="_blank">[94]</a>.</p><p><sup>9</sup> Interleukin-6.</p><p><sup>10</sup> Tumor necrosis factor-α.</p><p><sup>11</sup> bp: base pairs.</p><p><sup>12</sup> Cq: Quantification cycle.</p><p><sup>13</sup> Based on slaughter experiment.</p><p><sup>14</sup> Initial denaturation  = 10 min at 95°C; denaturation  = 30 s at 95°C; extension  = 30 s at 72°C, except for <i>TNF-α</i>, <i>PPAR</i>γ2, <i>PPAR</i>γ, <i>LEPR</i>, and <i>LEPRB</i> (60 s at 72°C).</p><p><sup>15</sup> Based on liver and s.c. fat biopsies from Trial 1.</p

    Longitudinal mRNA expression of genes related to insulin sensitivity in pluriparous cows during lactation I.

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    <p>Longitudinal mRNA expression of adiponectin receptors (ADIPOR1 and ADIPOR2) in liver and s.c. tail head biopsies of dairy cows together with adiponectin (ADIPOQ) and interleukin-6 (IL-6) mRNA abundance in s.c. adipose tissue and in liver of Trial 1, respectively. Cows were fed with conjugated linoleic acids (CLA, Lutrell® Pure, BASF SE, Ludwigshafen, Germany) at 100 g/day CLA or a control fat supplement (Silafat®, BASF SE) from day 1 until day 182 postpartum in Trial 1. Primiparous cow samples are not included (There are no sample in the CLA group at days 1, 70, 182, and 224) [Control: n = 10, CLA: n = 11]. Cumulative mRNA expression of both control and CLA is shown because CLA effect was insignificant. For normalisation, lipoprotein receptor-related protein 10 (LRP10), RNA Polymerase II (POLR2A) and eukariotic translation initiation factor 3 (EIF3K) in liver and LRP10, glyceraldehyde-phosphate-dehydrogenase (GAPDH), and POLR2A in s.c. adipose tissue were used as reference genes. Different letters indicate significant differences between days relative to parturition (<i>P</i>≤0.05; mean ± SEM). Area between vertical lines corresponds to the CLA supplementation period.</p

    Metabolic capacity of HeLa compared to A549.

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    <p>Fatty acid profiles were determined by GC-FID analysis of cellular lipid extracts and are expressed as % of total FAME. Data represent means ± SD.</p>a<p>Cells were incubated without (−) or with (+) 33 µM of C18∶2<i>n</i>−6 and C18∶3<i>n</i>−3, respectively, for 24 h. Both are substrates for FADS2.</p>b<p>Cells were incubated without (−) or with (+) 33 µM of the FADS2 products C18∶3<i>n</i>−6 and C18∶4<i>n</i>−3 for 24 h.</p>c<p>Considered Δ8-desaturation products.</p>d<p>Synthesis requires upstream a second step of <i>FADS2</i>-encoded activity.</p>e<p>After 72 h, significant increase in A459 to 2.7±0.2 when incubated with C18∶3<i>n</i>−3 (FA treatment×cell line: p = .002; see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115610#pone-0115610-g001" target="_blank">Figure 1B</a>, peak 14) and to 2.8±0.1 when incubated with C18∶4<i>n</i>−3 (FA treatment×cell line: p = .011).</p><p>Metabolic capacity of HeLa compared to A549.</p
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