17 research outputs found

    The Effect of Intra-Muscular Injections of Alpha-tocopherol on the Activity of Phospho- Fructokinase in the Slow- and Fast-Twitch Skeletal Muscles of Metabolic Stress-Induced and Malnourished Rats

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    It was been hypothesized that critical illnesswill change the activity of phospho-fructokinase (PFK)and that intramuscular (i.m) injections of theantioxidant ╬▒-tocopherol would protect this ratelimitingenzyme of glycolysis in the slow-twitch soleusand in the fast-twitch extensor digitorum longus (EDL)muscles of metabolically-stressed and malnourishedrats. Twenty eight male Wistar rats were divided intofour groups: controls (CNs), zymosan-injected (ZY), ╬▒-tocopherol-injected (ZYE), and pair-fed (PF). Rats werefed Osmolite-HN, and were sacrificed on day three. PFKactivity was measured in EDL and soleus muscles usinga fluorometric assay, and was found to be significantlylower in both the soleus and EDL of ZY rats. The PFKactivity in the EDL of ZYE rats was comparable to thePFK of PF rats and was significantly higher than ZYrats on day three. Glycolysis in the slow- and fast-twitchmuscle of ZY rats is adversely affected, and three daysof i.m injections of ╬▒-tocopherol significantly (p<0.05)protected PFK activity, in fast-twitch but not in slowtwitchmuscles