21 research outputs found

    Additional file 2: of The phospholipase DDHD1 as a new target in colorectal cancer therapy

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    Figure S1. DDHD1 silencing. To evaluate DDHD1 silencing a. Real-time PCR and b. Western blot analysis were performed on SW480, HCT116, HS5 and HUVEC transfected for 48 or 72 h with scrambled siRNA or DDHD1 siRNA. (TIFF 6629 kb

    Additional file 4: of The phospholipase DDHD1 as a new target in colorectal cancer therapy

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    Figure S2. Effects of DDHD1-expressing cells conditioned medium on DDHD1-silenced cell growth. Cell viability was measured by MTT assay on DDHD1-silenced SW480 cells in the presence of the conditioned medium (CM) of mock cells and DDHD1 overexpressing cells. (TIFF 3275 kb

    Effects of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray Extract on Adipocyte Differentiation of Human Mesenchymal Stem Cells

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    <div><p><i>Tithonia diversifolia</i> (Hemsl.) A. Gray (Asteraceae) is widely used in traditional medicine. There is increasing interest on the <i>in vivo</i> protective effects of natural compounds contained in plants against oxidative damage caused from reactive oxygen species. In the present study the total phenolic and flavonoid contents of aqueous, methanol and dichloromethane extracts of leaves of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray were determined; furthermore, free radical scavenging capacity of each extract and the ability of these extracts to inhibit <i>in vitro</i> plasma lipid peroxidation were also evaluated. Since oxidative stress may be involved in trasformation of pre-adipocytes into adipocytes, to test the hypothesis that <i>Tithonia</i> extract may also affect adipocyte differentiation, human mesenchymal stem cell cultures were treated with <i>Tithonia diversifolia</i> aqueous extract and cell viability, free radical levels, Oil-Red O staining and western bolt analysis for heme oxygenase and 5'-adenosine monophoshate-activated protein kinase were carried out. Results obtained in the present study provide evidence that <i>Tithonia diversifolia</i> (Hemsl.) A. Gray exhibits interesting health promoting properties, resulting both from its free radical scavenger capacity and also by induction of protective cellular systems involved in cellular stress defenses and in adipogenesis of mesenchymal cells.</p></div

    Total polyphenols and total flavonoids in three different extracts of leaves of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray.

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    <p>* = <i>p</i>< 0.001 vs aqueous extract;</p><p>** = <i>p</i>< 0.005 vs aqueous extract.</p><p>Total polyphenols and total flavonoids in three different extracts of leaves of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray.</p

    Determination of ROS by DCFH method in cultured hMSC: effect of aqueous extract of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray.

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    <p>Each value represents the mean ┬▒ S.D. of 5 experimental determinations. * = p< 0.001 <i>vs</i> the same cells cultured in the absence of plant extract (MSC control). Results are expressed as Fluorescence Intensity (F.I.) x 1 x 10<sup>6</sup>/mg prot).</p

    Representative Western bloting of HO-1 and pAMPK: effect of aqueous extract of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray on HO-1 and pAMPK expression in cultured hMSCs.

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    <p>Results, expressed as arbitrary units (A.U.), represent the mean ┬▒ S.D. of 5 experimental determinations. * = p< 0.005 with respect to tcontrol (C = hMSC control, T = hMSCs treated with 175 ╬╝mg/ml of aqueous extract of <i>Tithonia diversifolia</i> (Hemsl.) A. Gray).</p
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