38 research outputs found

    Low vitamin D diet increases the expression of vascular osteoblast differentiation factors in LDLR<sup>−/−</sup> mice.

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    <p>Panel A shows representative aortic cross sections immunohistochemically stained for Bmp2, Msx2 and Runx2 from LDLR<sup>−/−</sup> mice kept for 16 weeks on a <i>Western</i> diet with either low (50 IU/kg), recommended (1,000 IU/kg) or high (10,000 IU/kg) vitamin D<sub>3</sub> levels (×10). Panel B shows the percentage of stained areas relative to the total cross-section. Data are means ± SD of 12 mice per group. <sup>a,b,c</sup> Bars with different superscript letters differ, <i>P</i><0.015.</p

    VDR<sup>−/−</sup> mice have larger calcified lesion areas in their aortic valves than WT mice.

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    <p>Panel A shows representative von Kossa stained aortic root sections of VDR<sup>−/−</sup> mice fed a rescue diet, WT mice fed a rescue diet (WT<sub>RD</sub>) and WT mice fed a normal calcium/phosphorus diet (WT<sub>ND</sub>) for 8 weeks (×10). The bottom panel shows a magnification of the inserts in the top panel. Panel B shows the relative calcification area of the aortic sections and panel C the number of small-, medium- and large-sized calcified areas within an aortic cross-section. Data are means ± SD of 6–9 mice per group. <sup>a,b</sup> Bars with different superscript letters differ, <i>P</i><0.015.</p

    VDR knockout increases the expression of osteoblast differentiation factors in aortic valves.

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    <p>Panel A shows representative aortic root sections immunohistochemically stained for Bmp2, Msx2 and Runx2 from VDR<sup>−/−</sup> mice fed a rescue diet, WT mice fed a rescue diet (WT<sub>RD</sub>) and WT mice fed a normal calcium/phosphorus diet (WT<sub>ND</sub>) for 8 weeks (×10). Panel B shows the percentage of stained areas relative to the total cross-section. Data are means ± SD of 6–9 mice per group. <sup>a,b</sup> Bars with different superscript letters differ, <i>P</i><0.015.</p

    Vitamin D status of LDLR<sup>−/−</sup> mice in response dietary vitamin D levels.

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    <p>Plasma concentrations of 25(OH)D<sub>3</sub>, 24,25(OH)<sub>2</sub>D<sub>3</sub>, 1,25(OH)<sub>2</sub>D<sub>3</sub>, and relative mRNA abundancy of aortic calbindin D<sub>9 k</sub> of LDLR<sup>−/−</sup> mice fed a <i>Western</i> diet with low (50 IU/kg), recommended (1,000 IU/kg), or high (10,000 IU/kg) levels of vitamin D<sub>3</sub> for 16 weeks. Data are means ± SD of 4 probes per group (pools of 3 samples) for analysis of 1,25(OH)<sub>2</sub>D<sub>3</sub> and 12 mice per group for all other analyses. <sup>a,b,c</sup> Bars with different superscript letters differ, <i>P</i><0.05.</p

    Low vitamin D diet stimulates the development of aortic calcification in LDLR<sup>−/−</sup> mice.

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    <p>Panel A shows representative von Kossa stained aortic sections from LDLR<sup>−/−</sup> mice kept for 16 weeks on a <i>Western</i> diet with either low (50 IU/kg), recommended (1,000 IU/kg) or high (10,000 IU/kg) vitamin D<sub>3</sub> levels (×10). The bottom panel shows a magnification of the inserts in the top panel. Panel B shows the relative calcification area of the aortic sections and panel C the number of small-, medium- and large-sized calcified areas within an aortic cross-section. Data are means ± SD of 12 mice per group. <sup>a,b</sup> Bars with different superscript letters differ, <i>P</i><0.015.</p

    Egg weight and egg shell quality in response to UVB exposure and dietary vitamin D<sub>3</sub>.

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    <p>Effects of UVB exposure (UVB) and dietary vitamin D<sub>3</sub> (D<sub>3</sub>) on egg weight (A), egg shell thickness (B), and egg shell stability (C) over 4 weeks. Data in the panels represented means (n = 9). Data were analyzed by two-way ANOVA with the classification factors UVB exposure, vitamin D<sub>3</sub> in the diet, and the interaction between both factors. (A) No significant difference. (B) Effect of vitamin D<sub>3</sub> (week 2): <i>p</i><0.05, UVB × vitamin D<sub>3</sub> (week 2): <i>p</i><0.05. Effect of vitamin D<sub>3</sub> (week 3 and 4): <i>p</i><0.01, UVB × vitamin D<sub>3</sub> (week 3 and 4): <i>p</i><0.10. (C) Effect of UVB (week 2): <i>p</i><0.05. Effect of UVB (week 3): <i>p</i><0.10, vitamin D<sub>3</sub> (week 3): <i>p</i><0.10. Effect of UVB (week 4): <i>p</i><0.01, vitamin D<sub>3</sub> (week 4): <i>p</i><0.05, UVB × vitamin D<sub>3</sub> (week 4): <i>p</i><0.05. Data were additionally analyzed by paired <i>t</i>-test, *significantly different from baseline.</p
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