2 research outputs found

    Creation of a cell survival curve protocol for irradiated SiHa cells using a clinical linear accelerator

    No full text
    ilustraciones, fotograf铆as, gr谩ficasA finales del siglo XIX, poco despu茅s de la invenci贸n de los rayos X, se descubri贸 que la radiaci贸n ionizante pod铆a ser utilizada como tratamiento oncol贸gico. Durante el siguiente siglo se realizaron una serie de experimentos in vivo e in vitro, que buscaban explicar las propiedades de la interacci贸n radiaci贸n-c茅lula, creando el campo de la radiobiolog铆a. A partir de estas investigaciones se desarroll贸 el modelo lineal-cuadr谩tico, que sirve para cuantificar el da帽o letal y sub-letal de la radiaci贸n en la c茅lula, comparando la dosis recibida con la fracci贸n de supervivencia celular. Esto se conoce como una curva de supervivencia celular y es muy 煤til para comparar distintas caracter铆sticas de un tratamiento en radioterapia (como fraccionamiento, calidad de la radiaci贸n, entre otras). En este trabajo final de maestr铆a se busca crear un protocolo de construcci贸n de curvas de supervivencia celular utilizando haces de radiaci贸n de 6 MeV con un acelerador lineal para facilitar la realizaci贸n de experimentos radiobi贸logicos en el futuro. (Texto tomado de la fuente).At the end of the XIX century, right after the invention of xrays, it was discovered that ionizing radiation could be used as oncological treatment. During the following century a lot of advancements were made trying to understand that connection, in particular a lot of in vivo and in vitro experiments were made improving our understanding of the interaction of radiation and the cell, creating the field of Radiobiology. Using that research as a starting point the Lineal quadratic model was created, to attempt to quantify the lethal and sublethal damage produce to the cell by quantifying the cellular death with the dose prescribed. This is what is know as Cell Survival curve and it is very useful to define the reaction of tissue and cells after being expose to radiation. This study aims to develop a guide to create cell survival curves using what is known as an MTT assay for determining the impact of the radiation in the cell.Maestr铆aMag铆ster en F铆sica M茅dic

    Creation of a cell survival curve protocol for irradiated SiHa cells using a clinical linear accelerator

    No full text
    ilustraciones, fotograf铆as, gr谩ficasA finales del siglo XIX, poco despu茅s de la invenci贸n de los rayos X, se descubri贸 que la radiaci贸n ionizante pod铆a ser utilizada como tratamiento oncol贸gico. Durante el siguiente siglo se realizaron una serie de experimentos in vivo e in vitro, que buscaban explicar las propiedades de la interacci贸n radiaci贸n-c茅lula, creando el campo de la radiobiolog铆a. A partir de estas investigaciones se desarroll贸 el modelo lineal-cuadr谩tico, que sirve para cuantificar el da帽o letal y sub-letal de la radiaci贸n en la c茅lula, comparando la dosis recibida con la fracci贸n de supervivencia celular. Esto se conoce como una curva de supervivencia celular y es muy 煤til para comparar distintas caracter铆sticas de un tratamiento en radioterapia (como fraccionamiento, calidad de la radiaci贸n, entre otras). En este trabajo final de maestr铆a se busca crear un protocolo de construcci贸n de curvas de supervivencia celular utilizando haces de radiaci贸n de 6 MeV con un acelerador lineal para facilitar la realizaci贸n de experimentos radiobi贸logicos en el futuro. (Texto tomado de la fuente).At the end of the XIX century, right after the invention of xrays, it was discovered that ionizing radiation could be used as oncological treatment. During the following century a lot of advancements were made trying to understand that connection, in particular a lot of in vivo and in vitro experiments were made improving our understanding of the interaction of radiation and the cell, creating the field of Radiobiology. Using that research as a starting point the Lineal quadratic model was created, to attempt to quantify the lethal and sublethal damage produce to the cell by quantifying the cellular death with the dose prescribed. This is what is know as Cell Survival curve and it is very useful to define the reaction of tissue and cells after being expose to radiation. This study aims to develop a guide to create cell survival curves using what is known as an MTT assay for determining the impact of the radiation in the cell.Maestr铆aMag铆ster en F铆sica M茅dic
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