58 research outputs found

    Ann Trop Med Parasitol.

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    The isoenzyme profiles of a group of five Trypanosoma cruzi stocks obtained from congenital cases and of a second group of seven stocks obtained from chagasic mothers who did not transmit their infection congenitally were studied by electrophoresis of nine enzymes. All the mothers became infected in Bahia State (Brazil). With the exception of a triple 'heterozygous' GPI pattern, all T. cruzi stocks were identified as zymodeme 2 (Z2). The heterozygous pattern has not been recorded previously in Bahia. This study shows that enzymically similar T. cruzi stocks can show different behaviour with respect to transplacental transmission, and also with respect to clinicopathological presentation and therapeutic response of the congenital cases

    Adult T-cell leukemia/lymphoma

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2015-06-11T14:21:04Z No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de c√©lulas T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2015-06-11T14:35:17Z (GMT) No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de c√©lulas T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5)Made available in DSpace on 2015-06-11T14:35:17Z (GMT). No. of bitstreams: 1 Bittencourt, AL. Leucemis linfoma de c√©lulas T....pdf: 5046133 bytes, checksum: 8a1fb41a0749f476753dfee90473c995 (MD5) Previous issue date: 2008Universidade Federal da Bahia. Hospital Universit√°rio Prof. Edgard Santos. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Centro de Pesquisas Gon√ßalo Moniz. Laborat√≥rio de Patologia Experimental. Salvador, BA, BrasilResumo: A leucemia/linfoma de c√©lulas T do adulto (ATL) √© tipo agressivo de doen√ßa linfoproliferativa causada pelo v√≠rus linfotr√≥pico para c√©lulas T humanas (HTLV-I), geralmente fatal e que n√£o responde a quimioterapia. Classifica-se em formas aguda, cr√īnica, linfomatosa e indolente (smoldering). Outra forma cl√≠nica, a tumoral prim√°ria de pele, com caracter√≠sticas diferentes, foi sugerida recentemente. As formas aguda, linfomatosa e tumoral prim√°ria de pele s√£o as de pior progn√≥stico. Os crit√©rios diagn√≥sticos de ATL s√£o: sorologia positiva para o HTLV-I; diagn√≥stico citol√≥gico ou histol√≥gico de leucemia/linfoma de c√©lulas T, CD4+/CD25+; presen√ßa de linf√≥citos T anormais em sangue perif√©rico; confirma√ß√£o de integra√ß√£o monoclonal do DNA proviral do HTLV-I. H√° les√Ķes de pele em cerca de 70% dos casos, que podem ser prim√°rios (formas indolente e tumoral prim√°ria da pele) ou secund√°rios. As les√Ķes cut√Ęneas s√£o m√ļltiplas, sendo as mais freq√ľentes a eritrodermia, as p√°pulas e as placas. A ATL n√£o tem aspecto histol√≥gico caracter√≠stico, podendo apresentar padr√Ķes superpon√≠veis ao linfoma perif√©rico T n√£o especificado, √† micose fung√≥ide ou ao linfoma anapl√°sico de grandes c√©lulas. O padr√£o imuno-histoqu√≠mico pode tamb√©m simular o de outros tipos de linfoma T. Por esse motivo, √© muito importante que no Brasil seja solicitada sorologia para o HTLV-I em todos os casos de leucemia e/ou linfoma de c√©lulas T maduras.Abstract: Adult T cell leukemia/lymphoma (ATL) is an aggressive type of lymphoproliferative disease associated with the human T-cell lymphotropic virus type I (HTLV-I) that is characterized by a short survival time and absence of response to chemotherapy. ATL is classified into four clinical types: acute, chronic, lymphoma, and smoldering. Another clinical form of ATL, the primary cutaneous tumoral, with diverse characteristics, has been recently suggested. Patients with acute, lymphoma and primary cutaneous tumoral types have a poor prognosis. The diagnostic criteria of ATL consist of: positive serology for HTLV-I; cytologic or histologic confirmation of CD4+/CD25+ T-cell leukemia/lymphoma; abnormal T lymphocytes in peripheral blood; and confirmation of monoclonal integration of HTLV-I proviral DNA. There is skin involvement in around 70% of ATL cases, which could be primary (smoldering and primary cutaneous tumoral) or secondary. The skin lesions are multiple, erythroderma, papules and plaques being the most common. ATL has no characteristic histological pattern, and may present patterns that could superimpose nonspecific peripheral T-cell lymphoma, mycosis fungoides or anaplastic large cell lymphoma. The immunohistochemistry pattern may also be similar to that of other T-cell lymphomas. Thus, it is very important that in Brazil HTLV-I infection be investigated in all mature T-cell leukemias/lymphoma

    Microsatellite alterations are also present in the less aggressive types of adult T-cell leukemia-lymphoma

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    Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2016-04-12T17:41:31Z No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2016-04-12T17:51:50Z (GMT) No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5)Made available in DSpace on 2016-04-12T17:51:50Z (GMT). No. of bitstreams: 1 Magalhaes, M. Microsatellite alterations....pdf: 320318 bytes, checksum: f7780b37b7d568b38ab42107147e4d61 (MD5) Previous issue date: 2015Funda√ß√£o Oswaldo Cruz. Centro de Pesquisas Gon√ßalo Moniz. Laborat√≥rio de Patologia Experimental. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universit√°rio Professor Edgard Santos. Departamento de Dermatologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universit√°rio Professor Edgard Santos. Departamento de Patologia. Salvador, BA, BrasilFunda√ß√£o Oswaldo Cruz. Centro de Pesquisas Gon√ßalo Moniz. Laborat√≥rio de Patologia Experimental. Salvador, BA, Brasil / National Institute of Science and Technology of Tropical Diseases. INCT. Salvador, BA, BrasilBACKGROUND: Adult T-cell leukemia/lymphoma (ATL) is a mature T-cell neoplasia etiologically linked to HTLV-1. Manifestations of ATL are diverse and different clinical types with different tissue involvement and aggressiveness have been described. The mechanisms that lead to the development of ATL clinical types have not yet been clarified. Considering that in ATL patients HTLV-1 infection generally occurs in childhood, a multistep carcinogenesis model has been proposed. Microsatellite alterations are important genetic events in cancer development and these alterations have been reported in the aggressive types of ATL. Little is known about oncogenesis of the less aggressive types. METHODOLOGY/PRINCIPAL FINDINGS: In this study we investigated the role of the microsatellite alterations in the pathogenesis mediated by HTLV-1 in the different types of ATL. We examined the presence of microsatellite instability (MSI) and loss of heterozigosity (LOH) in matched pair samples (tumoral and normal) of 24 patients with less aggressive types (smoldering and chronic) and in aggressive types (acute and lymphoma) of ATL. Four microsatellite markers D10S190, D10S191, D1391 and DCC were analyzed. MSI was found in four patients, three smoldering and one chronic, and LOH in four patients, three smoldering and one acute. None of the smoldering patients with microsatellite alterations progressed to aggressive ATL. CONCLUSIONS/SIGNIFICANCE: To our knowledge, this is the first report describing the presence of MSI and LOH in the less aggressive types of ATL. These results indicate that microsatellite alterations may participate in the development of the less aggressive types of AT
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