24 research outputs found

    Hazai ny√°rak popul√°ci√≥genetikai √©s √©lettani vizsg√°lata az erd√©szeti c√©l√ļ felhaszn√°l√°s fejleszt√©se c√©lj√°b√≥l = Population genetic and physiological investigation on native poplars for development of silvicultural application

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    A p√°ly√°zati munka sor√°n 11 t√°jegys√©g 32 popul√°ci√≥j√°ban jel√∂lt√ľnk ki feh√©r, rezgŇĎ √©s sz√ľrke ny√°r mintaf√°kat. A mint√°zott 931 egyedbŇĎl 409-et vontunk be a genetikai vizsg√°latokba. Az elv√©gzett RAPD, PCR-RFLP √©s SSR vizsg√°latok sor√°n meg√°llap√≠tottuk, hogy a kiv√°lasztott markerek alkalmasak az alapfajok egyedeinek taxon√≥mia elk√ľl√∂n√≠t√©s√©re, az SSR vizsg√°lat pedig a hibridiz√°lts√°g m√©rt√©k√©nek meg√°llap√≠t√°s√°ra a sz√ľrke ny√°r eset√©ben. Az egyes popul√°ci√≥k genetikai diverzit√°s√°t kifejezŇĎ mutat√≥inak √∂sszevet√©se sor√°n meg√°llap√≠tottuk, hogy az alapfajok azon popul√°ci√≥i mutatj√°k a legmagasabb v√°ltozatoss√°got, amelyek az √∂kol√≥giai szempontb√≥l a faj optim√°lishoz k√∂zelebb √°ll√≥ termŇĎhelyein tal√°lhat√≥k. A molekul√°ris variancia forr√°sa valamennyi vizsg√°lati metodika eset√©ben a popul√°ci√≥kon bel√ľli v√°ltozatoss√°gra volt d√∂ntŇĎ m√©rt√©kben visszavezethetŇĎ. Az √∂sszehasonl√≠t√≥ n√∂v√©ny√©lettani vizsg√°latok c√©lja n√©gy ny√°r faj elt√©rŇĎ gy√∂keresed√©si hajlam√°nak a h√°tter√©ben √°ll√≥ okok √©s azok lehets√©ges befoly√°sol√°s√°nak a meg√°llap√≠t√°sa volt. A kl√≥nok k√∂z√∂tt a nem-struktur√°lis sz√©nhidr√°ttartalomban elt√©r√©s nem mutatkozott, nem befoly√°solta a gy√∂keresed√©st a dugv√°nyok eredeti helyzete √©s vastags√°ga sem. Az alkalmazott auxin kezel√©sek a gy√∂keresed√©st nem fokozt√°k, az auxin transzport g√°tl√°sa viszont jelentŇĎsen cs√∂kkentette. Pozit√≠v hat√°st az etil√©nszint√©zis fokoz√°s√°val √©rt√ľnk el. Az eredm√©nyek alapj√°n a ny√°r fajok k√∂z√∂tti gy√∂keresed√©si k√ľl√∂nbs√©g az elt√©rŇĎ etil√©n szintre vezethetŇĎ vissza. | In the frame of the research project 32 populations in 11 regions of Hungary were mapped during the last 4 years in order to inventory the genetic diversity of white poplar, trembling aspen and grey poplar. 409 individual out of the 931 trees sampled were analysed using RAPD, PCR-RFLP and SSR markers. We were able to distinguish between the three species with all the three marker types and the proportion of parent species in grey poplar individuals were also detected. Diversity indices showed the highest values in the populations which grow on ecologically optimal habitats. The Analysis of Molecular Variance (AMOVA) signed moderate variance among regions and relatively high proportion of diversity within populations. The objectives of our comparative physiological investigation on four poplar species were to determine the reasons for their different adventitious rooting abilities and to try to improve them. There were no significant differences in non-structural carbohydrate contents among the clones, and had no effect of the original position and diameter of cuttings on rooting. Root formation could not be enhanced by the applied auxin treatments however it was remarkably reduced by the inhibition of auxin transport. Enhancement in rooting could be only achieved by the increase of ethylene synthesis, accordingly the difference in rooting ability of poplar species can be attributed to the different ethylene level of cuttings

    Nemesny√°r kl√≥nok faanyagtani jellemzŇĎkh√∂z k√∂thetŇĎ g√©njeinek genetikai v√°ltozatoss√°ga = Allelic variation in candidate genes associated with wood properties of cultivated poplars

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    A nemesny√°rak kiemelkedŇĎ gazdas√°gi jelentŇĎs√©ggel b√≠rnak. A bemutatott vizsg√°lat legfŇĎbb c√©lja egy olyan kutat√°si metodika ismertet√©se, amely a faanyag tulajdons√°gai√©rt felelŇĎs kulcsenzimek k√≥dol√≥ r√©gi√≥inak azonos√≠t√°s√°b√≥l indul ki, bemutatva a genomikai alapokra helyezett nemes√≠t√©si technol√≥gi√°kban rejlŇĎ lehetŇĎs√©geket. A vizsg√°latunk elsŇĎ szakasz√°ban 24 k√ľl√∂nb√∂zŇĎ, a faanyagk√©pzŇĎd√©s szempontj√°b√≥l relev√°ns enzim k√≥dol√≥ r√©gi√≥j√°ra tervezt√ľnk primerp√°rokat. √Ėsszesen 55 saj√°t fejleszt√©sŇĪ primerp√°rt tesztelt√ľnk, 47,27%-os sikeress√©ggel. Ezut√°n nyolc enzimet v√°lasztottunk ki r√©szletesebb elemz√©sre h√©t ny√°rfaj √©s 11 hibrid kl√≥n bevon√°s√°val, √∂sszesen 23 ny√°r genot√≠pus vizsg√°lata r√©v√©n. A kiv√°lasztott enzimek egy r√©sze a lignifik√°ci√≥ folyamat√°ban vesz r√©szt (COMT, CCoAOMT, SAMS), egy m√°sik csoport a K+-f√ľggŇĎ xylogenezis sor√°n t√∂lt be kulcsszerepet (Kt, ptk2, SKOR), m√≠g a harmadik csoport (endo-1,4-b-xylanase, Araf-ase) a mikrofibrilla sz√∂g alakul√°s√°hoz k√∂thetŇĎ. A sikeresen amplifik√°lt √©s azonos√≠tott 13 markerr√©gi√≥ r√©v√©n √∂sszesen 188 szekvenci√°t elemezt√ľnk √©s 90 SNP-t azonos√≠tottunk. √Črt√©kelt√ľk a polimorf helyek sz√°m√°t, a nukleotid diverzit√°st, az inszerci√≥k/del√©ci√≥k sz√°m√°t, az SNP-k t√≠pus√°t, a rekombin√°ci√≥s esem√©nyek minim√°lis sz√°m√°t, illetve azonos√≠tottuk a konzervat√≠v szakaszokat. Eredm√©nyeink bemutat√°sa sor√°n r√©szletesen t√°rgyaljuk a vizsg√°latban rejlŇĎ alkalmaz√°si lehetŇĎs√©geket

    Review of investigation of variability of nad1 gene intron B/C of mitochondrial genome in Scots pine (Pinus sylvestris L.)

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    Background and Purpose: Scots pine (Pinus sylvestris L.) is the most extensively distributed pine, covering the whole Eurasian continent. The detection of genetic variability of Scots pine is of greate importance from the evolutionary point of view as well as for genetic conservation. Mitochondrial genome in Scots pine is maternally inherited and dispersed through seeds. Molecular studies have indicated high genetic diversity in Scots pine genome, allowing the detection of variation between and within population. Mitochondrial genome possesses variable regions in the gene encoded NADH dehydrogenase. The variable region is nad1 B/C intron. The different haplotypes of nad1 B/C intron allowed confirming the Iberian refugia in Holoecen and confirming the genetic diversity between different stands of Scots pine in Poland. The purpose of this study was to find out the genetic variability of nad1 B/C intron in two International Scots pine provenance experiments and native Scots pine provenance in Croatia in order to detect multiple origin and haplotype variation between provenances. Material and Methods: The samples of Scots pine were taken from two International experiments of provenances, one established in Croatia (22 provenances) and the other established in Hungary (20 provenances) and Croatian native provenance (Forest office Vrhovine, Forest administration Gospińá). The samples of needles were collected from randomly chosen trees of each provenance. Eight samples per provenance were collected for haplotype analyses. DNA was extracted and amplified with specific primers for nad1 B/C intron of the mitochondrial DNA. PCR products were analyzed using agarose gel electrophoresis and capillary electrophoresis on the bioanalyzer Agilent 2100. Results and Conclusion: We investigated samples from 42 provenances of Scots pine that originated from a broad range in Eurasia and were included in two international provenance experiments, and samples from native Croatian population. A total of 344 individual trees of Scots pine were analyzed. The datawere analyzed by agarose gel electrophoresis and by capillary electrophoresis. We did not detect variability in mitochondrial nad1 B/C region in analyzed samples. All analyzed samples were haplotype a. Based on our data, we could conclude that none of the tested samples originated from the Iberian glacial refugia. All tested samples could have the origin in glacial refugia placed in Mediterranean, Balkan region or in the northeastern Europe. More variable region of the nuclear DNA and themitochondrial DNA should be tested on a greater number of samples to obtain more informative data

    Review of investigation of variability of nad1 gene intron B/C of mitochondrial genome in Scots pine (Pinus sylvestris L.)

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    Background and Purpose: Scots pine (Pinus sylvestris L.) is the most extensively distributed pine, covering the whole Eurasian continent. The detection of genetic variability of Scots pine is of greate importance from the evolutionary point of view as well as for genetic conservation. Mitochondrial genome in Scots pine is maternally inherited and dispersed through seeds. Molecular studies have indicated high genetic diversity in Scots pine genome, allowing the detection of variation between and within population. Mitochondrial genome possesses variable regions in the gene encoded NADH dehydrogenase. The variable region is nad1 B/C intron. The different haplotypes of nad1 B/C intron allowed confirming the Iberian refugia in Holoecen and confirming the genetic diversity between different stands of Scots pine in Poland. The purpose of this study was to find out the genetic variability of nad1 B/C intron in two International Scots pine provenance experiments and native Scots pine provenance in Croatia in order to detect multiple origin and haplotype variation between provenances. Material and Methods: The samples of Scots pine were taken from two International experiments of provenances, one established in Croatia (22 provenances) and the other established in Hungary (20 provenances) and Croatian native provenance (Forest office Vrhovine, Forest administration Gospińá). The samples of needles were collected from randomly chosen trees of each provenance. Eight samples per provenance were collected for haplotype analyses. DNA was extracted and amplified with specific primers for nad1 B/C intron of the mitochondrial DNA. PCR products were analyzed using agarose gel electrophoresis and capillary electrophoresis on the bioanalyzer Agilent 2100. Results and Conclusion: We investigated samples from 42 provenances of Scots pine that originated from a broad range in Eurasia and were included in two international provenance experiments, and samples from native Croatian population. A total of 344 individual trees of Scots pine were analyzed. The datawere analyzed by agarose gel electrophoresis and by capillary electrophoresis. We did not detect variability in mitochondrial nad1 B/C region in analyzed samples. All analyzed samples were haplotype a. Based on our data, we could conclude that none of the tested samples originated from the Iberian glacial refugia. All tested samples could have the origin in glacial refugia placed in Mediterranean, Balkan region or in the northeastern Europe. More variable region of the nuclear DNA and themitochondrial DNA should be tested on a greater number of samples to obtain more informative data

    A faipari folyamatok szénlábnyom-elemzéséhez kapcsolódó logisztikai és energiafelhasználási konverziós faktorok megadása

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    A kl√≠mav√°ltoz√°s napjaink egyik kulcsfontoss√°g√ļ probl√©mak√∂re. Kialakul√°s√°nak egyik fŇĎ oka a l√©gk√∂rbe kibocs√°tott √ľvegh√°zhat√°s√ļ g√°zok mennyis√©ge, ami jelentŇĎs r√©szben ipari-logisztikai tev√©kenys√©gbŇĎl sz√°rmazik. Az √ľvegh√°zhat√°s√ļ g√°zok k√∂z√ľl a legfontosabb √©s egyben az egyik leggyakoribb a sz√©n-dioxid. A kl√≠mav√°ltoz√°s hat√°sainak cs√∂kkent√©se √©rdek√©ben, teh√°t ismerni kell a sz√©n-dioxid keletkez√©s√©nek helyeit, mennyis√©g√©t √©s a k√∂rnyezetre gyakorolt hat√°sait. A kutat√°s c√©lja a faipari folyamatok sz√©nl√°bnyom-meghat√°roz√°s√°t szolg√°l√≥ √ļn. konverzi√≥s faktorok megad√°sa, majd egy egys√©gesen alkalmazhat√≥ sz√°m√≠t√°si m√≥dszertan kidolgoz√°sa. A szerzŇĎk az irodalmi kutat√≥munka sor√°n vizsg√°lt√°k a k√∂z√ļti sz√°ll√≠t√°sb√≥l √©s a faipari v√°llalatok villamos-, √©s hŇĎfelhaszn√°l√°s√°b√≥l sz√°rmaz√≥ sz√©n-dioxid-kibocs√°t√°sokat, majd megadt√°k az ezeket jellemzŇĎ konverzi√≥s faktorokat sz√©n-dioxid-egyenr√©t√©kben (CO2e) kifejezve. A bemutatott m√≥dszertan a gyakorlat sz√°m√°ra adapt√°lhat√≥ √©s hozz√°j√°rul a v√°llalati sz√©n-dioxidkibocs√°t√°s szisztematikus √©s tudom√°nyos alapokon nyugv√≥ meghat√°roz√°s√°hoz

    Sz√°razs√°gtŇĪr√©sben szerepet j√°tsz√≥ SNP-k azonos√≠t√°sa kocs√°nytalan t√∂lgy popul√°ci√≥kban : alapkutat√°si eredm√©nyek a fenntarthat√≥ t√∂lgygazd√°lkod√°s√©rt = Identification of SNP markers responsible for drought tolerance in sessile oak populations : results of basic research for sustainable oak management

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    √Āllom√°nyalkot√≥ fafajaink genetikai √°llom√°nyban fellelhetŇĎek az alkalmazkod√°si folyamatok lenyomatai, amelyek fontos ismereteket szolg√°ltathatnak a kl√≠maadapt√°ci√≥t seg√≠tŇĎ erdŇĎmŇĪvel√©si strat√©gi√°k meghat√°roz√°s√°hoz. Jelen kutat√°sunk sor√°n 18 k√∂z√©p- √©s d√©lkelet-eur√≥pai kocs√°nytalan t√∂lgy [Quercus petraea (Matt.) Liebl.] popul√°ci√≥ egyedeiben kimutatott SNP-k (egypontos nukleotid elt√©r√©sek) √©s z√°rvatermŇĎ n√∂v√©nyek genomj√°ban meghat√°rozott, sz√°razs√°gtŇĪr√©ssel kapcsolatba hozhat√≥ szekvenci√°k kocs√°nyos t√∂lgy (Quercus robur L.) referencia genomra t√∂rt√©nŇĎ t√©rk√©pez√©s√©t v√©gezt√ľk el, meghat√°rozva √≠gy azon SNP-k csoportj√°t, amelyek kapcsolatba hozhat√≥k a sz√°razs√°gi stresszv√°laszban szerepet j√°tsz√≥ folyamatokkal. A kiugr√≥ FST √©rt√©kkel b√≠r√≥ marker l√≥kuszok √©s √∂sszesen 94 klimatikus v√°ltoz√≥ bevon√°s√°val v√©gzett regresszi√≥anal√≠zis sor√°n 16 erŇĎsen szignifik√°ns kapcsolatot mutattunk ki egyes g√©nhelyek √©s n√©h√°ny, csapad√©k vagy hŇĎm√©rs√©klet alap√ļ k√∂rnyezeti v√°ltoz√≥ k√∂z√∂tt. A sz√°razs√°g szelekci√≥ alatt √°ll√≥ SNP-k kimutat√°sa sz√°mos tov√°bbi, a fenntarthat√≥ t√∂lgygazd√°lkod√°st megalapoz√≥ kutat√°s alapj√°t k√©pezheti a j√∂vŇĎben. = The genetic information concerning the adaptation of main tree species to different environmental conditions could provide considerable knowledge to determine forest management responses to climate change. In the present study, we carried out a parallel mapping of SNP markers revealed in 18 Middle- and Southeast-European sessile oak [Quercus petraea (Matt.) Liebl.] populations and EST sequences of stress-responsive loci downloaded from an EST repository to determine the group of those SNPs, which are associated with the genetic background of adaptation processes in oaks. Regression analysis revealed 16 significant correlations between four outlier SNP loci representing high FST values and 94 climatic variables. All variables with significant correlations were found to be related to precipitation or temperature. The stress-responsive loci identified in this study may serve as a basis for common research to support future sustainable management of sessile oak in Hungary
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