9,594 research outputs found

    Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo

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    The TGF-beta-related molecule Nodal plays an essential and conserved role in left-right patterning of the vertebrate embryo. Previous reports have shown that the zebrafish and mouse Cerberus-related proteins Charon and Cerberus-like-2 (Cerl-2), respectively, act in the node region to prevent the Nodal signal from crossing to the right side, whereas chick Cerberus (cCer) has an unclear function in the left-side mesoderm. In this study, we investigate the transcriptional regulation and function of cCer in left-right development. By analyzing the enhancer activity of cCer 5' genomic sequences in electroporated chick embryos, we identified a cCer left-side enhancer that contains two FoxH1 and one SMAD binding site. We show that these Nodal-responsive elements are necessary and sufficient for the activation of transcription in the left-side mesoderm. In transgenic mouse embryos, cCer regulatory sequences behave as in chick embryos, suggesting that the cis-regulatory sequences of Cerberus-related genes have diverged during vertebrate evolution. Moreover, our findings from cCer overexpression and knockdown experiments indicate that cCer is a negative-feedback regulator of Nodal asymmetric signaling. We propose that cCer and mouse Cerl-2 have evolved distinct regulatory mechanisms but retained a conserved function in left-right development, which is to restrict Nodal activity to the left side of the embryo.info:eu-repo/semantics/publishedVersio

    Idosos ativos, idosos saud√°veis

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    Mestrado em FisioterapiaIntrodu√ß√£o: O envelhecimento saud√°vel enfatiza uma abordagem que reconhece os direitos √† igualdade de oportunidades e de tratamento. A falta de atividade f√≠sica contribui para muitas doen√ßas cr√≥nicas que afetam os idosos. Objetivo: O objetivo geral deste projeto √© planear um programa de atividade f√≠sica segundo as suas necessidades e prefer√™ncias da popula√ß√£o dos Olivais Sul tendo em conta a qualidade de vida. M√©todo: Para concretizar este projeto foi desenhado um estudo observacional, descritivo e transversal. Apresenta como vari√°vel dependente a qualidade de vida e como vari√°vel independente a atividade f√≠sica. Foi ainda, elaborado um question√°rio e utilizada a escala de qualidade de vida SF-36 e os dados analisados atrav√©s do programa IBM SPSS Statistics 20. Resultados: A dimens√£o da qualidade de vida mais afetada √© a vitalidade, seguida da dor corporal, fun√ß√£o f√≠sica e sa√ļde geral, desempenhos f√≠sico e emocional, mudan√ßa de sa√ļde, sa√ļde mental e fun√ß√£o social. A maioria dos idosos expressa o desejo de praticar mais atividade f√≠sica. Conclus√£o: √Č poss√≠vel concluir que alguns dos problemas presentes na popula√ß√£o afetam a qualidade de vida e que, segundo diversos estudos, podem ser melhorados com a atividade f√≠sica. Este projeto torna-se pertinente pois a maioria dos idosos expressa desejo de praticar mais atividade f√≠sica e apresenta condi√ß√Ķes que podem ser melhoradas com a sua pr√°tica, nomeadamente problemas respirat√≥rios, card√≠acos e neurol√≥gicos; dores; diminui√ß√£o da for√ßa muscular, equil√≠brio e flexibilidade.ABSTRACT - Introduction: Healthy aging emphasizes an approach that recognizes the rights to equal opportunity and treatment. The lack of physical activity contributes to many chronic diseases that affect older people. Objetives: The goal of this project is to plan a program of physical activity according the needs, preferences and the quality of life of the population resident in Olivais Sul Methodology: To realize this project was designed an observational, descriptive and transversal study. The dependent variable is the quality of life and the independent variable is the physical activity. It also was designed a questionnaire and used a scale for measure the quality of life, SF-36- The data analyze was using the software PASW Statistics 20. Results: The dimension of quality of life more affected is the vitality, followed by body pain, physical function and general health, physical and emotional performance, changes in health, mental health and social function. Most seniors wants to practice more physical activity. Conclusion: It is possible to conclude that some of the problems present in this population affect the quality of life and, somes studies say, that they may be improved with physical activity. This project is pertinent because most seniors expressed desire to do more physical activity and provides conditions that can be improved with this practice, including respiratory problems, cardiac and neurological disorders, pain, decreased muscle strength, balance and flexibility

    Unfairness in consumer services: Outcomes of differential treatment of new and existing clients

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    In a consumption context, there is a growing interest in understanding unfair behaviour of firms towards customers. Our research focuses on unfairness perceptions driven by differential treatment, particularly through price discrimination, i.e. the practice of charging differential prices to different customers. Our purpose is to investigate the consequences of these practices for unfairness perceptions, satisfaction, trust and patronage, showing a dual perspective: the perceptions of new vs existing clients when they face the advantaged or disadvantaged conditions. A survey-based experimental design approach was used. We conclude that unfairness perception is stronger for existing than for new clients, prompting negative attitudinal and behavioural consequences when the former are exposed to disadvantaged conditions in relation to the latter. Our study aims to provide marketers with a perspective on the pitfalls related to differential treatment between present and prospective clients, with implications in terms of design and implementation of customer management strategies

    Avaliação da comunidade microbiana diazotrófica em solos sob cultura biológica por métodos moleculares

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    Dissertação mest., Biologia Marinha, Universidade do Algarve, 200

    Pessoas cegas no mercado de trabalho: da formação à integração

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    Disserta√ß√£o de mestrado integrado em PsicologiaO mercado de trabalho evoluiu ao longo dos tempos, lado a lado com a evolu√ß√£o tecnol√≥gica, legislativa e com uma aposta na forma√ß√£o profissional, abrindo as portas √† integra√ß√£o de pessoas cegas. Assim, este estudo tem como principal objetivo perceber como se processa a integra√ß√£o de indiv√≠duos cegos no mercado de trabalho, tanto na perspetiva dos pr√≥prios, como na das respetivas organiza√ß√Ķes. Para alcan√ßar os objetivos recorreu-se a um estudo com metodologia qualitativa, tendo sido entrevistados 13 elementos, 8 pessoas cegas integradas no mercado de trabalho, com licenciatura ou com forma√ß√£o profissional da Associa√ß√£o dos Cegos e Ambl√≠opes de Portugal (ACAPO) e 5 chefias. Com este estudo concluiu-se que os/as entrevistados/as consideram que atualmente a integra√ß√£o de pessoas cegas no mercado de trabalho √© positiva, havendo, contudo, ainda um longo caminho a percorrer, nomeadamente, melhorar a informa√ß√£o junto dos empregadores, acerca desta tem√°tica. Outra conclus√£o prende-se com o facto dos dados sugerirem que os conte√ļdos lecionados pelas associa√ß√Ķes formativas deveriam acompanhar as necessidades atuais do mercado de trabalho.The job market has evolved over time, as much as in their perspective as in that of the organizations side by side with the technological and legislative evolution and a commitment to the professional training, paving the way for the integration of blind people. Thus, the main goal of this study is to understand how blind people get integrated into the job market, as much in their perspective as in the organization‚Äôs. To reach this goal, it was developed a qualitative study in which 13 people were interviewed, 8 blind people integrated into the job market, with a university degree or a professional training from the Portuguese Association for the Blind and Visually Impaired (ACAPO) and 5 supervisors. With this study, it was concluded that the respondents consider that nowadays the integration of blind people into the job market is positive, even though there is still a long way to go, particularly to improve the information that the employers have about this topic. Another conclusion is related to the fact that the data suggests that the contents lectured by the training associations should keep up with the current needs of the job market

    Prevalence of the causes of anemia in dogs

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    Disserta√ß√£o de Mestrado Integrado em Medicina Veterin√°riaThis retrospective study aimed to assess the causes and severity of anemia in dogs and their relative significance and evaluate possible diagnostic insights. The veterinary medical database of the Professor M. Bra√ßo Forte Clinical Analysis Laboratory at the Faculty of Veterinary Medicine, University of Lisbon (FVM-ULisbon), Lisbon, Portugal, was searched. Dogs with anemia (hematocrit < 37%) were included and assigned to different groups. Dogs less than a year old and samples with lipemia, blood clots, hemolysis, and jaundice were excluded. The age and sex of the dog, the severity and regeneration of anemia, and the final diagnosis were all evaluated in the case reports. The case records of 465 dogs with low hematocrit were included. Cancer-related anemia accounted for 21,94% of the cases, and anemia of inflammatory disease for 15,27%. Specific causes of anemia, such as females with anemia of inflammatory disease (p<0,001) and young dogs with infectious disease-related anemia (p<0,001), were significantly associated with sex and age, respectively. Non-regenerative anemias were found to be mild (56,98%) to moderate (31,84%) and more likely to occur in dogs with renal disease-related anemia (p=0,001) rather than in dogs with infectious disease-related anemia (p<0,001). Overall, anemia severity tended to be mild to moderate (83,44%), except for immune-mediated anemia that was tendentially severe (60%). This study allowed the identification of the most common causes of anemia at the Veterinary Teaching Hospital of the FVM-ULisbon, where cancer-related anemia and anemia of inflammatory disease were the most frequently identified causes. In addition, it was also found that the severity of anemia may provide diagnostic information, in that severe anemia is more often linked to regenerative anemia such as immune-mediated anemia and mild to moderate anemia to non-regenerative anemia. However, a more extensive study should be conducted to understand whether the information gained in this study extends nationwide and better understand the pathophysiological mechanisms implicated in the different causes of anemia.RESUMO - Este estudo retrospetivo visou avaliar as causas e a gravidade da anemia em c√£es e o seu significado relativo e avaliar poss√≠veis hip√≥teses diagn√≥sticas. A base de dados m√©dico-veterin√°ria do Laborat√≥rio de An√°lises Cl√≠nicas do Professor M. Bra√ßo Forte da Faculdade de Medicina Veterin√°ria da Universidade de Lisboa (FMV-ULisboa), Lisboa, Portugal, foi pesquisada. Os c√£es com anemia (hemat√≥crito < 37%) foram inclu√≠dos e atribu√≠dos a diferentes grupos. Foram exclu√≠dos os c√£es com menos de um ano de idade e as amostras com lip√©mia, co√°gulos de sangue e icter√≠cia. A idade e sexo do c√£o, a gravidade e regenera√ß√£o da anemia, e o diagn√≥stico final foram todos avaliados nos relat√≥rios dos casos. Foram inclu√≠dos os registos dos casos de 465 c√£es com hemat√≥crito baixo. A anemia relacionada com o cancro representou 21,94% dos casos, e a anemia por doen√ßa inflamat√≥ria 15,27%. Causas espec√≠ficas de anemia, como a anemia por doen√ßa inflamat√≥ria em f√™meas (p<0,001) e a anemia relacionada com doen√ßa infeciosa em c√£es jovens (p<0,001), relacionam-se com o sexo e a idade, respetivamente. Verificou-se que as anemias n√£o-regenerativas eram leves (56,98%) a moderadas (31,84%) e mais suscet√≠veis de ocorrer em c√£es com anemia relacionada com doen√ßas renais (p=0,01) do que em c√£es com anemia relacionada com doen√ßa infeciosa (p<0,001). No geral, a gravidade da anemia tende a ser ligeira a moderada (83,44%), exceto no caso da anemia hemol√≠tica imunomediada que √© tendencialmente grave (60%). Este estudo permitiu a identifica√ß√£o das causas mais comuns de anemia no Hospital Escolar Veterin√°rio da FMV-ULisboa, onde a anemia relacionada com o cancro e a anemia devida a doen√ßas inflamat√≥rias foram as causas mais frequentemente identificadas. Al√©m disso, verificou-se tamb√©m que a gravidade da anemia pode fornecer informa√ß√Ķes de diagn√≥stico, na medida em que a anemia grave est√° mais frequentemente ligada √† anemia regenerativa, como a anemia hemol√≠tica imunomediada e a anemia ligeira a moderada √† anemia n√£o regenerativa. Contudo, deveria ser realizado um estudo mais extensivo para compreender se a informa√ß√£o obtida neste estudo se estende a todo o pa√≠s e compreender melhor os mecanismos fisiopatol√≥gicos implicados nas diferentes causas de anemia.N/

    what role for central banking and supervisors to build a climate and environmental resilient financial system

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    O conceito de ‚ÄėFinan√ßas Verdes‚Äô surgiu como uma resposta √† necessidade de compatibilizar a economia global com os esfor√ßos para combater as altera√ß√Ķes clim√°ticas e assim, prosseguir um desenvolvimento ambientalmente sustent√°vel. Para o sistema financeiro, esta abordagem implica tratar dos riscos financeiros relacionados com o clima, que resultam dos impactos adversos das altera√ß√Ķes clim√°ticas, assim como das externalidades negativas decorrentes da transi√ß√£o para uma economia neutra na emiss√£o de carbono. No entanto, os bancos centrais e os quadros regulamentares existentes negligenciaram at√© ent√£o estas fontes de risco, resultando numa aus√™ncia de informa√ß√£o financeira e n√£ofinanceira relativa √† exposi√ß√£o dos bancos a riscos clim√°ticos e numa potencial falta de credibilidade em produtos financeiros verdes e sustent√°veis. Esta pesquisa visa definir, caracterizar e analisar os riscos financeiros relacionados com o clima no sector banc√°rio, incluindo os desafios de adapta√ß√£o das carteiras de empr√©stimos aos fatores de transmiss√£o micro e macroecon√≥micos de riscos f√≠sicos e transit√≥rios. Em particular, centra-se no papel da regula√ß√£o e supervis√£o financeira, bem como dos bancos centrais no panorama internacional e, em particular, da Uni√£o Europeia, a fim de assegurar um sistema financeiro s√≥lido e est√°vel face aos crescentes impactos adversos das altera√ß√Ķes clim√°ticas, nomeadamente na transforma√ß√£o dos fundamentos econ√≥micos e na mudan√ßa das prefer√™ncias dos consumidores em dire√ß√£o a uma economia sustent√°vel. Esse papel poder√° implicar um alargamento dos mandatos dos supervisores financeiros e dos bancos centrais a fim de considerarem fatores de sustentabilidade nas suas tomadas de decis√£o. Seguindo uma abordagem tradicional baseada no risco, analisam-se os principais instrumentos micro e macroprudenciais √† luz dos tr√™s pilares dos Acordos de Basileia, bem como poss√≠veis altera√ß√Ķes √† pol√≠tica monet√°ria e aos seus instrumentos financeiros tradicionais.The concept of "Green Finance" emerged as a response to the need of aligning the global economy with the efforts to fight climate change and thus, pursuing an environmentally sustainable development. For the financial system, this includes addressing climate-related financial risks, which arise from the adverse impacts of climate change, and the negative externalities of the transition to a carbonneutral economy. However, central banks and the existing regulatory frameworks have traditionally neglected these sources of risk, resulting in a lack of financial and non-financial information on banks' exposure to climate risks and a lack of trust in green and sustainable financial products. This research aims to define, characterise and analyse climate-related financial risks in the banking sector, including the challenges in adapting lending portfolios to physical and transitional micro and macroeconomic transmission channels. In particular, it focuses on the role of financial regulation and supervision, as well as central banks, in the international landscape (especially in the context of the European Union) to ensure a sound and stable financial system in light of the rising negative effects of climate change, the changing economic fundamentals, and shifting consumer preferences towards a sustainable economy. Furthermore, supervisors and central banks may be persuaded to prioritise sustainability factors in their decision-making processes, which may entail an extension of their mandates. Using a traditional risk-based approach, key micro and macroprudential instruments are analysed in the light of the three pillars of the Basel Accords, as well as possible changes to monetary policy and its traditional financial instruments

    Online grocery shopping In Portugal: how store-based businesses can adapt to win

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    This paper examines the current state of online grocery shopping in Portugal, in order to help existing store-based grocers provide the best online offer that they can. Review on existing studies and an analysis of customer preferences in Portugal helped understand that Price and Convenience are the key factors to win in the online grocery channel. Given the early stage of adoption of this service, this means that retailers have improvement opportunities through which they can adapt their offers and increase sales, especially in what concerns order fulfilment
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