396 research outputs found

    Target and non-target approaches for food authenticity and traceability

    Get PDF
    The author acknowledges the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020).info:eu-repo/semantics/publishedVersio

    Identificação da origem botânica do mel por DNA barcoding

    Get PDF
    O mel e um produto natural muito apreciado pelas suas propriedades sensoriais, nutricionais e medicinais. Os méis monoflorais são produtos de valor acrescentado por serem considerados de elevada qualidade e com aroma e sabor bem definidos, sendo por isso susceptíveis a adulterações. Tal facto torna importante o desenvolvimento de novas metodologias para a avaliação da autenticidade e origem botânica. 0 método usado atualmente para a determinação da origem botânica baseia-se na analise melissopalinol6gica, que e morosa e requer técnicos especializados [1]. Os métodos de ADN apresentam-se como alternativas promissoras para a identificação de espécies em matrizes complexas e processadas.info:eu-repo/semantics/publishedVersio

    Hypericum species identification to assess the authenticity of plant food supplements

    Get PDF
    In the last years, medicinal plants and derived products have become increasingly available in the EU market as ingredients of formulations sold as food supplements. This type of products is legally considered as food under the Directive 2002/46/EC [l], thus with legal responsibility of its safety relying on business operators as they are not under the control of the European Medicines Agency (EMA).This work has been supported by FCT through grant PEst-C/EQB/LA0006/2013 and project EXPL/DTP-SAP/1438/2013 (Safety of PIant Food Supplements: searching for adulterant pharmaceutlcal drugs and plants)info:eu-repo/semantics/publishedVersio

    Autenticação de produtos cárneos com a designação Halal: Deteção e quantificação de derivados de suíno (Sus scrofa)

    Get PDF
    Devido aos recentes escândalos alimentares relacionados com adulterações em produtos cárneos, tem-se assistido a uma maior atenção por parte dos consumidores e autorida-des sobre a ocorrência de fraudes neste setor, especialmen-te no que respeita a substituição de carne de espécies ani-mais de valor elevado por proteínas musculares de mais baixo custo. Em particular, devido ao seu baixo preço e ele-vada disponibilidade, a carne de porco e/ou derivados de suíno (gordura, plasma, colagénio, entre outros) podem ser fraudulentamente adicionados em produtos cárneos, tendo por objetivo o aumento de lucros de fabricantes pouco es-crupulosos [1,2]. Para além destas práticas representarem uma fraude económica, a presença de espécies animais não declaradas na rotulagem é algo que causa elevada preocu-pação em certos grupos religiosos para os quais o consumo de determinadas espécies é proibido.Os autores agradecem o apoio financeiro da Fundação para a Ciência e a Tecnologia (FCT) através do projeto PEst-C/EQB/LA0006/2013.info:eu-repo/semantics/publishedVersio

    Towards authentication of Korean ginseng-containing foods: differentiation of five Panax species by a novel diagnostic tool

    Get PDF
    Panax ginseng C.A. Meyer (Korean ginseng) is one of the most valuable medicinal plants, recognised for its neuroprotection and other beneficial health effects. It is present in a wide range of food products, namely plant food supplements (PFS) and herbal infusions. However, other Panax species, having distinct therapeutic effects, are also known as ginseng, pointing out the need of authenticating such products. The present work aims at proposing a new high-resolution melting (HRM) method to differentiate five Panax species (P. ginseng, P. quinquefolius, P. notoginseng, P. japonicus and P. trifolius), targeting the gene encoding the dammarenediol synthase, involved in the biosynthesis pathway of ginsenosides. A Panax-specific real-time PCR assay was successfully developed with high analytical performance parameters (Efficiency = 100.5 %, R2 = 0.995, dynamic range 10 ng-1 pg of ginseng DNA). Panax DNA was detected in 17 out of 23 ginseng-containing commercial foods, including herbal infusions and PFS. For the first time, HRM analysis differentiated five Panax species with high level of confidence (>98 %), which corroborated sequencing data. Fourteen products were successfully clustered, being all except one in accordance with their labelling statements. Therefore, the present work proposes a reliable and high-throughput tool to authenticate ginseng products that could be useful for control laboratories.The authors acknowledge the support of project UIDB 00690/2020 funded by FCT/MCTES through national funds. The authors are grateful for the supply of leaves from the Botanical Garden of Edinburgh (Edinburgh, Scotland), Botanical Garden of University of Porto (Porto, Portugal), Botanical garden of UTAD (Vila Real, Portugal), as well as to the voucher seeds from the RBG (Kew, Ardingly, West Sussex, UK), USDA-ARS Germplasm (Beltsville, MD, USA) and NCRPIS/Iowa State university (Ames, IA, USA).info:eu-repo/semantics/publishedVersio

    Tracing Styphnolobium japonicum (syn: Sophora japonica) as a potential adulterant of ginkgo-containing foods by real-time PCR

    Get PDF
    The rising demand for ginkgo-containing products and their high economic value make them desirable targets for adulteration, particularly by the partial substitution with other plant species. Styphnolobium japonicum (plant rich in flavonol glycosides) is known as a potential adulterant of ginkgo-based foods. Therefore, this work aimed at developing a species-specific real-time polymerase chain reaction (qPCR) method for the identification/quantification of S. japonicum as an adulterant of ginkgo-containing products. The method used the EvaGreen dye, targeting the internal transcribed spacer 2 (ITS2) region of S. japonicum, providing acceptable performance parameters and a sensitivity down to 0.02 pg of DNA. Moreover, a qPCR assay was established using binary mixtures of S. japonicum in G. biloba, covering the dynamic range of 50−0.05% (w/w) of added adulterant. After trueness evaluation with blind samples, the approach was applied to 21 commercial herbal infusions, from which one was positive to S. japonicum, but below the limit of quantification (0.05 %), suggesting its inadvertent contamination rather than adulteration. To the best of our knowledge, for the first time, a specific method was proposed to quantify potential adulterations of G. biloba products with S. japonicum, providing an accurate and cost-effective tool to authenticate ginkgo-containing herbal foods.The work was supported through the projects UIDB/50006/2020 and UIDB/00690/2020, funded by FCT/MCTES (Fundação para a Ciˆencia e Tecnologia and Minist´erio da Ciˆencia, Tecnologia e Ensino Superior) through national funds. L. Grazina thanks FCT and ESF (European Social Fund) through POCH (Programa Operacional Capital Humano) for her PhD grant SFRH/BD/132462/2017. J. Costa thanks FCT for funding through program DL 57/2016 – Norma transitória (SFRH/BPD/102404/2014).info:eu-repo/semantics/publishedVersio

    Authentication of ginkgo biloba herbal products by a novel quantitative real-time PCR approach

    Get PDF
    Ginkgo biloba is a widely used medicinal plant. Due to its potential therapeutic effects, it is an ingredient in several herbal products, such as plant infusions and plant food supplements (PFS). Currently, ginkgo is one of the most popular botanicals used in PFS. Due to their popularity and high cost, ginkgo-containing products are prone to be fraudulently substituted by other plant species. Therefore, this work aimed at developing a method for G. biloba detection and quantification. A new internal transcribe spacer (ITS) marker was identified, allowing the development of a ginkgo-specific real-time polymerase chain reaction (PCR) assay targeting the ITS region, with high specificity and sensitivity, down to 0.02 pg of DNA. Additionally, a normalized real-time PCR approach using the delta cycle quantification (ΔCq) method was proposed for the effective quantification of ginkgo in plant mixtures. The method exhibited high performance parameters, namely PCR efficiency, coefficient of correlation and covered dynamic range (50-0.01%), achieving limits of detection and quantification of 0.01% (w/w) of ginkgo in tea plant (Camellia sinensis). The quantitative approach was successfully validated with blind mixtures and further applied to commercial ginkgo-containing herbal infusions. The estimated ginkgo contents of plant mixture samples suggest adulterations due to reduction or almost elimination of ginkgo. In this work, useful and robust tools were proposed to detect/quantify ginkgo in herbal products, which suggests the need for a more effective and stricter control of such products.This work was supported by FCT (Fundação para a Ciência e Tecnologia) under the Partnership Agreements UIDB 50006/2020 and UIDB 00690/2020. L. Grazina is grateful to FCT grant (SFRH/BD/132462/2017) financed by POPH-QREN (subsidised by FSE and MCTES).info:eu-repo/semantics/publishedVersio

    Adulteration of Dietary Supplements by the Illegal Addition of Synthetic Drugs: a review

    Get PDF
    In the last few years, the consumption of dietary supplements, especially those having plants as ingredients, has been increasing due to the common idea that they are natural products posing no risks to human health. In the European Union and the United States, dietary supplements are legally considered as foods/special category of foods, thus are not being submitted to any safety assessment prior to their commercialization. Among the issues that can affect safety, adulteration by the illegal addition of pharmaceutical substances or their analogs is of major concern since unscrupulous producers can falsify these products to provide for quick effects and to increase sales. This review discusses the various classes of synthetic drugs most frequently described as being illegally added to dietary supplements marketed for weight loss, muscle building/sport performance and sexual performance enhancement. Information regarding regulation and consumption is also presented. Finally, several conventional and advanced analytical techniques used to detect and identify different adulterants in dietary supplements and therefore also in foods, with particular emphasis on plant food supplements, are critically described. This review demonstrates that dietary supplement adulteration is an emerging food safety problem and that an effective control by food regulatory authorities is needed to safeguard consumers.This work was supported by European Union (FEDER funds through COMPETE) and Natl. Funds (FCT, Fundação para a Ciência e Tecnologia) through projects EXPL/DTP-SAP/1438/2013 (Safety of plant food supplements: searching for adulterant pharmaceutical drugs and plants) and LAQV UID/QUI/50006/2013.info:eu-repo/semantics/publishedVersio

    Food supplements adulteration with undeclared synthetic phosphodiesterase type-5 drugs (PDE-5) inhibitors

    Get PDF
    The use ofplant food supplements (PFS) in developed countries is becoming increasingly popular mostly due to the consumers' widespread idea that natural products are safer and healthier than conventional pharmaceutical drugs [l]. However, in the last years, several studies have been showing the existence of gaps in PFS regulation that can result in insuíficient quality control and intentional adulteration ofthese products [2]. Among the issues that can aíFect PFS safety, the illegal addition of pharmaceutical substances is of major concern, since unscrupulous producers can dope PFS to provide for quick effects. Supplements used for sexual performance improvement are among the most popular PFS used by males. One of the major concerns in this type of product is the possible adulteration with drugs used for the treatment of erectile dysfunction, namely synthetic phosphodiesterase type-5 (PDE-5) inhibitor drugs, such as sildenafil, vardenafil and tadalafil which are approved prescription drugs being marketed as Viagra (Pfizer, USA), Levitra (Bayer Pharmaceuticals Co., Germany) and Cialis (Elli Lilly, USA), respectively. Since these drugs can present side eífects, its illegal addition in PFS can seriously endanger consumers' health. In particular, individuais for whom conventional PDE-5 inhibitor drugs are contraindicated and consequently use food supplements as an alternative maybe at risk ifthe clandestine addition ofthese drugs occurs. In the last years, the presence ofthis type ofdrugs have been detected by FDA in the US, and reported in food supplements commercialized in Ásia, however studies regarding this issue in the EU are almost inexistent. For the presented reasons, an analytical methodology based on the use of high performance liquid chromatography coupled to a fluorescence detector in series with a photodiode array (HPLC-FL-PDA) is proposed for the detection ofPDE-5 inhibitors, namely sildenafil and its analogue acetildenafil, vardenafil and tadalaíil, illegally added to supplements used for aphrodisiac purposes.to FCT (PEst-C/EQB/LA0006/2013 and EXPL/DTP-SAP/1438/2013) and QREN (NORTE-07-0124-FEDER-000069-CIÊNCIA DO ALIMENTO).info:eu-repo/semantics/publishedVersio
    • …
    corecore