167 research outputs found

    Influence of chemical and physical conditions on the production of bacteriocin by Aeromonas hydrophila

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    Aeromonas hydrophila have been isolated as a cause of a cute gastroenteritis in 23 (5.6%) of 410 patients. Other bacterial enteropathogens have been isolated from 387 patients with diarrhea, were 19 different strains. A. hydrophila occurred more commonly in children with acute diarrhea, the results showed that 18(78.26%) isolates of A. hydrophila found in children under 10 years old ,distributed to 10(43.47%) in male and 8(34.78%) in female ,and in adults with diarrhea 5 (21.73%). In the other hand, we noticed frequency of isolation was higher in male 14(60.86%) when compared with 9(39.14%) in female. Six strains of A. hydrophila have been observed to have bacteriocin activity against 12 of 23 different A. hydrophila ,as well as Staphylococcus aureas, Pseudomonas aeruginosa, Escherichia coli, Enterobacter cloacea and Shigella dysenteria. The results showed Bacteriocin-like substances (BLS11) had isoinhibitory activity on 10 same A. hydrophila species and heteroinhibitory activity effects on all pathogenic bacterial strains used, while BLS5 showed isoinhibitory activity on 2 same A. hydrophila species and heteroinhibitory activity by effecting on gram negative only, and BLS3& BLS12 showed activity on E. coli isolates only, and none of BLS1& BLS10(isoinhibitory activity on 1 A.hydrophila respectively) had effect on all pathogenic bacteria. Among the standard laboratory media used Brain Heart Infusion broth (BHI) showed the maximum production and poor yields resulted from growth in Peptone Glyserol (PG) and Nutrient broth. We selected BLS11 to their wide range effect on same species and enteric pathogenic strains, to study the Influence of chemical and physical conditions on the production of BLS by A.hydrophila. The BLS11 preparations from A.hydrophila11 strains of A. hydrophila were tolerant to all three treatments of surfactant. In the other hand, effect of organic acid on BLS production BLS11 has been studied and showed no remarkable difference in zone of inhibition when used acetone as affecter element, while both of isopropanol and ethanol have narrow inhibition zone range when compared with control strain. These results indicated that most A. hydrophila might be harboring plasmid mediated bacteriocin like substance, and there are no relation between BLS production and number of plasmid bands present in bacteria

    Synthesizing, Structuring, and Characterizing Bioactivities of .Cr(III), La(III), and Ce(III) .Complexes with Nitrogen, Oxygen .and Sulpher donor bidentate Schiff base ligands

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    تم أستخدام قاعدتا شف ، وهما: 3- (بنزيلدين أمينو)-6-مثيل -2-ثايوكسو-2,5-داي هايدرو بيرميدين-4-(3H)-أون(LS), و 3- (بنزيلدين أمينو)-6-مثيل بيرميدين-4-3H),(5 H داي أون(LA),كاليكاندات مخلبية لتحضير بعض المعقدات مع أيونات Cr(III) و La(III) وCe(III).معقدات العناصر الثلاثية مع قواعد شف(LA) و(LS) تم تشخيصها عن طريق الطرائق الفيزيائية الكيميائية(تحليل المعادنF. A.A,والتحليل الدقيق للعناصرC.H.N.Sوأطياف الأشعة تحت الحمراءFTIRوالأشعة الفوق البنفسجية-المرئيةUV–visوقياسات الحساسية المغناطيسية و التوصلية). و وفقًا لهذه النتائج ، تم اقتراح شكل ثماني السطوح مع معقدCr(III)في حين أعطت المعقدات La(III) و Ce(III)أشكالا متشابهة و ذات عدد تناسقي سبعة. تم فحص قواعد شف ومعقداتها المخلبية المعدنية في المختبرin vitro لتقييم قدرتها على التثبيط ضد (Pseudonomousaerugionosa) سالبة الصبغة و (Staphylococcus aureus) كبكتيريا موجبة الصبغة. كما تم دراسة أقل تركيز يحدث عنده التثبيط (MIC) لتحديد التركيز المنخفض للتثبيط. تم اختيار المضادات الحيوية (Amoxcillin و Ampicillin) لمقارنة فعاليتها مع المركبات الجديدة. كما تم دراسة الفعالية المضادة للفطريات ضد نوعين من الفطريات (PenicillumSpp.) و (Aspergillus flavus) لجميع المركبات. أظهرت نتائج المركبات المحضرة نشاطًا مضادًا للبكتيريا أفضل من الأدوية.Two Schiff bases, namely, 3-(benzylidene amino) -2-thioxo-6-methyl 2,5-dihydropyrimidine-4(3H)-one (LS])and 3-(benzylidene amino)-6-methyl pyrimidine 4(3H, 5H)-dione(LA)as chelating ligands), were used to prepare some complexes of Cr(III), La(III), and Ce(III)] ions. Standard physico-chemical procedures including metal analysis M%, element microanalysis (C.H.N.S) , magnetic susceptibility, conductometric measurements, FT-IR and UV-visible Spectra were used to identify Metal (III) complexes and  Schiff bases (LS) and (LA). According to findings, a [Cr(III) complex] showed six coordinated octahedral geometry, while [La(III), and Ce(III) complexes]were structured with coordination number seven.  Schiff's bases and mineral complexes were examined in vitro to investigate potential inhibition against Gram-positive bacteria such as Pseudonomous aerugionosa and Gram-negative bacteria such as Staphylococcus aureus. The low concentration for inhibition has been also determined by studying the minimal inhibitory concentrations MIC .Antibiotics (Ampicillin, Amoxicillin) have been chosen to contrast their activity. Furthermore, Anti-fungal activity against two types of fungi ʺAspergillus flavusʺ and ʺPenicillum Spp.ʺ was studied for these compounds. The results of the antibacterial activity were better compared to the standard drugs

    Virtual data integration for a clinical decision support systems

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    Clinical decision support (CDS) supplies clinicians and their patients, and relevant staff with meaningful and timely information intelligently integrated or visualized to enhance health and the health sector. Data is the backbone of decision support systems, especially (clinical) ones. Data integration (either virtual or physical manner) is a powerful technique to manipulate a vast amount of heterogeneous data and prepare it as input for the decision-making process. The difficulties in manipulating data that have a physical data integration technique motivated the decision support developers to tend to data virtualization as a data integration technique. In this paper, a clinical decision support system was developed using the virtual data integration technique. The developed system was evaluated in terms of usability and its capability of providing clinical decision support. The evaluation findings indicate that the proposed system is highly usable and has a positive impact on supporting the clinical decision-making process

    In Situ Hybridization for Molecular Detection of Human Papilloma Viral 6 / 11 DNA in Adenoctomized Tissues from A group of Iraqi Pediatric Patients

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    هنالك أكثر من 200 من الأنماط الجينية لفايروس الورمي الحليمي البشري والتي تم التعرف عليها من خلال  الترابط مابين الانماط الجينية ذات الاختطار الواطئ للفايروس الحليمي البشري مع مجموعة متنوعة من الأورام الفموية والبلعوم والبلعوم الأنفي وكذلك الأورام الحليمية غير الخبيثة  وتضخم الغدة. هدفت هذه الدراسة  من نوع الاستقصائية- السيطرة إلى تحديد الحمض النووي للفيروس الورمي الحليمي البشري 6/11 في انسجة الغدد البلعومية الأنفية واللوزتين لمجموعة من المرضى الذين يخضعون لاستئصال اللوزتين. تم الحصول على 60عينة من أنسجة اللوزتين الأنفية البلعومية من الأطفال الذين يعانون من تضخم اللوزتين الغدي, تضمنت 40 من أنسجة اللوزتين الأنفية البلعومية للمرضى الذين يعانون من تضخم غدي ، و 20 عينة نسجية من الأطفال  بعد عمليات التشذيب للانسجة الأنفية السفلية بدون  تغييرات مرضية ملحوظة (كمجموعة  سيطرة). تم اجراء الكشف الجزيئي عن الحامض النووي للفيروس الورمي الحليمي البشري نمط 6\11 باستخدام تقنية التهجين الموضعي ذات الحساسية العالية في عينات الأنسجة الضخمة الغدية ز تم العثور على 8 من أصل 40 تحتوي على نتائج إيجابية للحمض النووي للفيروس الحليمي البشري 6\11 حيث شكلت 20 ٪ من إجمالي الأنسجة الغدية البلعومية الأنفية.بينما لاتوجد أي اشارة موجبة للحامض النووي للفايروس الحليمي البشري نمط 6\11 في مجموعة السيطرة. أظهر التحليل الإحصائي للنتائج في هذا البحث اختلافًا كبيرًا مقارنةً  مابين مجموعتي المرضى والسيطرة عند مستوى احتمالية اقل او يساوي 5%. يمكن أن يلعب فيروس الورم الحليمي البشري ذات الاختطار الواطئ في تلك الأنسجة الغدية المتضخمة دورًا في التسبب في الأمراض و / أو يشكل عامل  لانتشار العدوى الفيروسية للانسجة الاخرىAmong more than 200 different human papilloma viral genotypes, the association of low oncogenic risk-HPV genotypes have been recognized with a variety of oral, oropharyngeal, nasopharyngeal benign tumors as well as non-neoplastic polyposis and papillomas and adenoid hypertrophy. This prospective case- control study aims to determine the rate of DNA detection of HPV genotype 6/11 in nasopharyngeal adeno- tonsillar tissues from a group of patients subjected to adenoctomy for adenoid hypertrophy . A total number of nasopharyngeal adeno-tonsillar tissue specimens from pediatric patients with adenoid hypertrophy were enrolled; 40 nasopharyngeal adeno-tonsillar tissues from patients with adenoid hypertrophy, and 20 normal nasal tissue specimens were obtained from pediatric patients following trimming operations of their inferior nasal turbinates' with unremarkable pathological changes (as an apparently healthy control group). The molecular detection methods for HPV detection were performed by using DNA probes via a recent version of chromogenic in situ hybridization specified for low- risk HPV genotypes.  Among total adenoid hypertrophied tissue specimens group, 8 out of 40 were found to contain positive results for DNA of HPV 6 / 11 genotype, constituting 20% of the total screened nasopharyngeal adenotonsillar tissues. No positive– CISH reactions were detected in the control nasal tissues. The statistical analysis of results in this research showed significant difference when compared to the control apparently healthy tissues.   The significant rate of low- oncogenic HPV genotypes detection in those adenoid hypertrophied tissues could play, in part, a role in their pathogenesis and / or constituting a herald focus for the spread of such important virally transmitted infection

    Enhancing Heat Transfer Performance In Simulated Fischer–Tropsch Fluidized Bed Reactor Through Tubes Ends Modifications

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    Fluidized bed reactors are essential in a wide range of industrial applications, encompassing processes such as Fischer–Tropsch synthesis and catalytic cracking. The optimization of performance and reduction in energy consumption in these reactors necessitate the use of efficient heat transfer mechanisms. The present work examines the considerable impact of tube end geometries, superficial gas velocity, and radial position on heat transfer coefficients within fluidized bed reactors. It was found that the tapered tube end configurations have been empirically proven to improve energy efficiency in fluidized bed reactors significantly. For example, at a superficial gas velocity of 0.4 m/s, the tapered end form\u27s local heat transfer coefficient (LHTC) demonstrated a significant 20% enhancement compared to the flat end shape. The results and findings of this work make a valuable contribution to the advancement of complex models, enhance the efficiency of fluidized bed reactor processes, and encourage further investigation into novel tube geometries

    The gamma half-Cauchy distribution: Properties and applications

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    A new distribution, namely, the Gamma-Half-Cauchy distribution is proposed. Various properties of the Gamma-Half-Cauchy distribution are studied in detail such as limiting behavior, moments, mean deviations and Shannon entropy. The model parameters are estimated by the method of maximum likelihood and the observed information matrix is obtained. Two data sets are used to illustrate the applications of Gamma-Half-Cauchy distribution

    Cell specific apoptosis by RLX is mediated by NFκB in human colon carcinoma HCT-116 cells

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    BACKGROUND: Resistance to chemotherapy represents a major obstacle in correcting colorectal carcinomas (CRC). Inspite of recent advances in the treatment of metastatic disease, the prognosis of the patients remains poor. RLX, a vasicinone analogue has been reported to possess potent bronchodilator, anti-asthmatic and anti-inflammatory properties. However, its anti-cancer activity is unknown. RESULTS: Here, we report for the first time that RLX has anti-cancer property against panel of human cancer cell lines and most potent activity was found against HCT-116 cells with IC(50) value of 12 μM and have further investigated the involvement of NFκB and caspase-3 in RLX action in CRC apoptosis. Following RLX and BEZ-235 treatment in HCT-116, we observed significant down-regulation of NFκB (1 to 0.1 fold) and up-regulation of caspase-3 (1 to 2 fold) protein expressions. Additionally, morphological studies revealed membrane blebbing, cell shrinkage, chromatin condensation and finally apoptosis in HCT-116 cells. CONCLUSIONS: Overall, these findings indicate that RLX is a potent small molecule which triggers apoptosis, and promising potential candidate to be a chemotherapeutic agent

    CO-INOCULATION WITH RHIZOBIUM AND BACILLUS SP TO IMPROVE THE PHOSPHORUS AVAILABILITY AND YIELD OF WHEAT (Triticum aestivum L.)

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    ABSTRACT Intensive cropping has resulted-in wide spread deficiency of nutrients in most of the soils and situation is becoming more serious because of a increase in the use of high priced chemical fertilizers and their negative influence on the environment. Exploitation of biological intervention mainly phosphate solublizing bacteria (PSB) has attracted great attention, as they have enormous potential in providing soil phosphorus for plant growth, by increasing the availability of accumulated phosphate through solubilization. A field experiment was conducted to investigate the effect of Rhizobium and Bacillus, alone and in combination on the yield parameters of wheat. Uniform dose of N and K (160 and 60 kg ha ), grain protein (11.84%) and 1000 grain weight (62 g) were higher in co-inoculation of Rhizobium and Bacillus. It was also recorded that coinoculation of Rhizobium and Bacillus improved the grain yield up to 17.5% as compared to control. In single inoculation Bacillus gave better result and showed an increase of 7.7% in grain yield. Phosphorus uptake by grains (25.29 kg ha -1 ) was maximized by co-inoculation followed by Bacillus inoculation. Available phosphorus in post harvest sample of soil was recorded (16.27 mg kg -1 ) which was significantly higher than all other treatments. Results clearly demonstrated that co-inoculation of Rhizobium and Bacillus sp enhanced the availability of phosphorus and exert positive effect on the growth and yield of crop
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