The use of a carbodiimide-containing fixative for the immunohistochemical demonstration of coagulation factor VIII in rat vascular tissue

Immunohistochemically, coagulation F-VIII-R:AG in vascular endothelial cells can be demonstrated with antihuman F-VIII-R:AG antisera after fixation with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide, using both peroxidase and fluorescence techniques. The generally used aldehyde containing fixatives, result in a loss of immunoreactivity. Carbodiimide is preferable as a fixative for the cellular localization of F-VIII-R:AG. Chemicals/CAS: blood clotting factor 8, 9001-27-8; cyanamide, 151-51-9, 420-04-2; Ethyldimethylaminopropyl Carbodiimide, 1892-57-5; Factor VIII, 9001-27-8; Fixative