319 research outputs found
The Role of Pathology in Small Renal Mass Laparoscopic Cryoablation
Objective. We evaluated histological outcome of intraoperative biopsies at laparoscopic renal mass cryoablation (LCA), prevalence of peritumoral fat tissue invasion, and risk of tract seeding. Methods. Patients were biopsied 3–5 times (16-gauge). Histology was analyzed by general pathologists and reviewed. Peritumoral fat was histologically examined. The trocar used for biopsy-guidance was examined by cytology. Records were studied for reporting tract metastasis. Results. 77 biopsied renal masses with mean ± SD diameter 30 ± 7.4 mm were histologically classified by primary and review pathology revealing 64 and 62 malignancies, 13 and 15 benign lesions, respectively. In 30/34, the fat covered a carcinoma but revealed no malignancy. Cytology showed no malignant cells but was inconclusive in 1 case. No tract metastasis occurred. Conclusions. The use of an intraoperative biopsy protocol provides histological diagnosis of all renal masses. No existence of peritumoral fat tissue invasion or tract seeding was found
Effect of bone decalcification procedures on DNA in situ hybridization and comparative genomic hybridization. EDTA is highly preferable to a routinely used acid decalcifier
Decalcification is routinely performed for histological studies of
bone-containing tissue. Although DNA in situ hybridization (ISH) and
comparative genomic hybridization (CGH) have been successfully employed on
archival material, little has been reported on the use of these techniques
on archival decalcified bony material. In this study we compared the
effects of two commonly used decalcifiers, i.e. , one proprietary,
acid-based agent (RDO) and one chelating agent (EDTA), in relation to
subsequent DNA ISH and CGH to bony tissues (two normal vertebrae, six
prostate tumor bone metastases with one sample decalcified by both EDTA
and RDO). We found that RDO-decalcified tissue was not suited for DNA ISH
in tissue sections with centromere-specific probes, whereas we were able
to adequately determine the chromosomal status of EDTA-decalcified
material of both control and tumor material. Gel electrophoresis revealed
that no DNA could be successfully retrieved from RDO-treated material.
Moreover, in contrast to RDO-decalcified tumor material, we detected
several chromosomal imbalances in the EDTA-decalcified tumor tissue by CGH
analysis. Furthermore, it was possible to determine the DNA ploidy status
of EDTA- but not of RDO-decalcified material by DNA flow cytometry.
Decalcification of bony samples by EDTA is highly recommended for
application in DNA ISH and CGH techniques
Interphase cytogenetics and comparative genomic hybridization of human epithelial cancers and precursor lesions
Pancreatic fibrosis correlates with exocrine pancreatic insufficiency after pancreatoduodenectomy
Molecular cytogenetic evaluation of gastric cardia adenocarcinoma and precursor lesions
Analyses of cancer incidence data in the United States and Western Europe
revealed steadily rising rates over the past decades of adenocarcinomas of
the esophagus and gastric cardia. Genetic information on gastric cardia
adenocarcinoma and its preneoplasias is sparse. We have used comparative
genomic hybridization to obtain a genome-wide overview of 20 archival
gastric cardia adenocarcinomas and 10 adjacent preneoplastic lesions (
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