Estudios meióticos en Agalinis fiebrigii y Agalinis genistifolia (Orobanchaceae)

Páez, Valeria de los A.; Aldo R. Andrada; Graciela E. Ruiz de Bigliardo. 2015. “Estudios meióticos en Agalinis fiebrigii y Agalinis genistifolia (Orobanchaceae)”. Lilloa 52 (2). Agalinis es un género americano y sus escasos antecedentes citogenéticos coinciden en que las especies de Norteamérica presentan números básicos x = 13, 14 mientras que las especies sudamericanas x = 16. En este trabajo se analiza la meiosis y se estima la viabilidad del polen de A. fiebrigii y A. genistifolia, las únicas dos especies que se distribuyen en el Noroeste Argentino. El número gametofítico observado en ambas especies de n = 16 es consistente con el número básico x = 16 presente en los taxones de Sudamérica. Durante la meiosis se observaron irregularidades y cuerpos extranucleares, que son reportados por primera vez en Agalinis

Comparative cytogenetics of Auchenorrhyncha (Hemiptera, Homoptera): a review

A comprehensive review of cytogenetic features is provided for the large hemipteran suborder Auche norrhyncha, which currently contains approximately 42,000 valid species. This review is based on the analysis of 819 species, 483 genera, and 31 families representing all presently recognized Auchenorrhyn cha superfamilies, e.i. Cicadoidea (cicadas), Cercopoidea (spittle bugs), Membracoidea (leafhoppers and treehoppers), Myerslopioidea (ground-dwelling leafhoppers), and Fulgoroidea (planthoppers). History and present status of chromosome studies are described, as well as the structure of chromosomes, chro mosome counts, trends and mechanisms of evolution of karyotypes and sex determining systems, their variation at different taxonomic levels and most characteristic (modal) states, occurrence of partheno genesis, polyploidy, B-chromosomes and chromosome rearrangements, and methods used for cytoge netic analysis of Auchenorrhyncha.info:eu-repo/semantics/publishedVersio

Estudios citogenéticos en Steirastoma brevis Sulcer (Coleoptera, Cerambycidae)

Se investigan por primera vez los cromosomas meióticos de Steirastoma brevis Sulcer. Los resultados muestran que el diploide es 2n = 28 y el haploide n = 13 AA + Xyp. El bivalente sexual presenta heteropicnosis positiva durante las diferentes etapas meióticas. El cromocentro grande suele estar asociado con la heterocromatina de los autosomas durante la interfase premeiótica hasta su completa separación. Los datos sobre la frecuencia del quiasma sugieren que es baja en esta especie

Karyotype and male pre-reductional meiosis of the sharpshooter Tapajosa rubromarginata (Hemiptera: Cicadellidae)

Cicadellidae in one of the best represented families in the Neotropical Region, and the tribe Proconiini comprises most of the xylem-feeding insects, including the majority of the known vectors of xylem-born phytopathogenic organisms. The cytogenetics of the Proconiini remains largely unexplored. We studied males of Tapajosa rubromarginata (Signoret) collected at El Manantial (Tucumán, Argentina) on native spontaneous vegetation where Sorghum halepense predominates. Conventional cytogenetic techniques were used in order to describe the karyotype and male meiosis of this sharpshooter. T. rubromarginata has a male karyological formula of 2n=21 and a sex chromosome system XO:XX (♂:♀). The chromosomes do not have a primary constriction, being holokinetic and the meiosis is pre-reductional, showing similar behavior both for autosomes and sex chromosomes during anaphase I. For this stage, chromosomes are parallel to the acromatic spindle with kinetic activities in the telomeres. They segregate reductionally in the anaphase I, and towards the equator during the second division of the meiosis. This is the first contribution to cytogenetic aspects on proconines sharpshooters, particularly on this economic relevant Auchenorrhyncha species. Rev. Biol. Trop. 59 (1): 309-314. Epub 2011 March 01

Análisis de la meiosis y viabilidad del polen en morfotipos intraespecíficos de Cuscuta paradiana (Cuscutaceae)

En la familia Cuscutaceae, el género Cuscuta está representado por plantas holoparásitas con distribución cosmopolita. El taxón incluye tres subgéneros: Cuscuta, Mono- gyna y Grammica ; este último muy representado en América. En este trabajo se exponen la morfología floral, estudios meióticos y viabilidad del polen de ejemplares colectados en cuatro (4) poblaciones de Cuscuta parodiana del subgénero Grammica del Noroeste Argentino. El análisis de la morfología floral permitió diferenciar 4 morfotipos en base a los caracteres de la corola y el gineceo. De las entidades estudiadas tres fueron tetraploides (n = 30) y uno de ellos diploide (n = 15). Durante la meiosis se han observado configuraciones trivalentes y tetravalentes, puentes de cromatina, cromosomas fuera de placa metafásica, citomixis y cuerpos redondeados extranucleares

Chromosome numbers in insects of Argentina I. Cytogenetic characterization of 15 species with economic importance.

In the present study, the somatic and gametic chromosome numbers in fifteen species of insects from Argentina are reported. Said species are characterized for their economic importance as pest of crops or as agricultural pest controllers. The species belonged to the following orders and families: Neuroptera, Chrysopidae: Ceraeochrysa cincta (n = 5 + XY), Ceraeochrysa paraguaria (n = 5 + XY), Chrysoperla argentina (n = 5 + XY), Chrysoperla asoralis (n = 5 + XY), Chrysoperla externa (n = 5 + XY), Leucochrysa cruentata (n = 7 + XY) y Plesiochrysa elongate (n = 5 + XY), Coleoptera, Coccinellidae: Cyclonela sanguinea (n = 9 + XYp), Eriopis connexa (n = 9 + XYp), Harmonia axyridis (n = 7 + XYp), Hippodamia convergens (n = 9 + XYp), y Melyridae: Astylus atromaculatus (n = 8 + XYp), Dermaptera, Forficulidae: Doru lineare (2n = 20) and D. luteipes (2n = 20), Diptera, Ulidiidae: Euxesta eluta (n = 5 + XY). Chromosomal information for six species and additional gametic chromosome number for Euxesta eluta are established for the first time. In addition, a new chromosome number is cited for Astylus atromaculatus that is different to the one previously reported

Molecular characterization of wdr68 gene in embryonic development of Xenopus laevis

WDR68, also known as DCAF7, is a WD40 repeated domain protein highly conserved in eukaryotic organisms in both plants and animals. This protein participates in numerous cellular processes and exerts its function through interactionwith other proteins. In the present work, we isolated, sequenced and characterized cDNA corresponding to the wdr68 gene in embryos of the amphibian Xenopus laevis. Syntenic analysis revealed high conservation of the genomicregion containing the WDR68 locus in amniotes. Nevertheless, in fishes and amphibians, we observed that the tandem genes surrounding wdr68 undergoes certain rearrangements with respect to the organization found in amniotes. We also defined the temporal and spatial expression pattern of the wdr68 gene in the development of Xenopus laevis through whole mount in situ hybridization and RT-PCR techniques. We observed that wdr68 is ubiquitously expressed during early embryonic development but, during the neurula stage, it undergoes a strong expression in the neural tube and in the migratory cephalic streams of the neural crest. At the tailbud stages, it is strongly expressed in the cephalic region, particularly in otic and optic vesicles, in addition to branchial arches. In contrast, wdr68 transcripts are localized in the somitic mesoderm in the trunk. The expression area that includes the migratory neural crest of the head and the branchial arches suggest that this gene would be involved in jaws formation, probably through a hierarchical relationship with the component genes of the endothelin-1/endothelin receptor type A cell signaling pathway.Fil: Bonano, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Martín, Eduardo. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Moreno Ruiz Holgado, Maria Macarena. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; ArgentinaFil: Silenzi Usandivaras, Gabriela María. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ruiz de Bigliardo, Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Aybar, Manuel Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentin

Números cromosómicos en insectos de Argentina. I. Caracterización citogenética en 15 especies de importancia económica

In the present study, the somatic and gametic chromosome numbers in fifteen species of insects from Argentina are reported. Said species are characterized for their economic importance as pest of crops or as agricultural pest controllers. The species belonged to the following orders and families: Neuroptera, Chrysopidae: Ceraeochrysa cincta (n = 5 + XY), Ceraeochrysa paraguaria (n = 5 + XY), Chrysoperla argentina (n = 5 + XY), Chrysoperla asoralis (n = 5 + XY), Chrysoperla externa (n = 5 + XY), Leucochrysa cruentata (n = 7 + XY) y Plesiochrysa elongate (n = 5 + XY), Coleoptera, Coccinellidae: Cyclonela sanguinea (n = 9 + XYp), Eriopis connexa (n = 9 + XYp), Harmonia axyridis (n = 7 + XYp), Hippodamia convergens (n = 9 + XYp), y Melyridae: Astylus atromaculatus (n = 8 + XYp), Dermaptera, Forficulidae: Doru lineare (2n = 20) and D. luteipes (2n = 20), Diptera, Ulidiidae: Euxesta eluta (n = 5 + XY). Chromosomal information for six species and additional gametic chromosome number for Euxesta eluta are established for the first time. In addition, a new chromosome number is cited for Astylus atromaculatus that is different to the one previously reported.En el presente trabajo se informan los números cromosómicos somáticos y/o gaméticos de 15 especies de insectos presentes en Argentina, que se caracterizan por su importancia económica como plagas de cultivos o controladores de plagas agrícolas, pertenecientes a los siguientes órdenes y familias: Neuroptera, Chrysopidae: Ceraeochrysa cincta (n = 5 + XY), Ceraeochrysa paraguaria (n = 5 + XY), Chrysoperla argentina (n = 5 + XY), Chrysoperla asoralis (n = 5 + XY) , Chrysoperla externa (n = 5 + XY), Leucochrysa cruentata (n = 7 + XY) y Plesiochrysa elongata (n = 5 + XY), Coleoptera, Coccinellidae: Cycloneda sanguinea (n = 9 + XYp), Eriopis connexa (n = 9 + XYp), Harmonia axyridis (n = 7 + XYp), Hippodamia convergens (n = 9 + XYp) y Melyridae: Astylus atromaculatus (n = 8 + XYp), Dermaptera, Forficulidae: Doru lineare (2n = 20), D. luteipes (2n = 20) y Diptera, Ulidiidae: Euxesta eluta (n = 5 + XY). Por primera vez, se cita información cromosómica para seis especies, incluyendo un número cromosómico gamético adicional para Euxesta eluta. Además, se da a conocer en Astylus atromaculatus un número cromosómico diferente a los recuentos previamente reportados

Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)

During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.Fil: Silenzi Usandivaras, Gabriela Maria. Fundación Miguel Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Álvarez, R. E.. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Andrada, Aldo Rubén. Fundación Miguel Lillo; ArgentinaFil: Oviedo, Andrea Verónica de Fátima. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Moreno Ruiz Holgado, Maria Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ruiz de Bigliardo, Graciela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina. Fundación Miguel Lillo; ArgentinaFil: Romero Sueldo, Gladys Mabel. Fundación Miguel Lillo; ArgentinaXXXV Annual Scientific Meeting of the Tucuman Biology AssociationSan Miguel de TucumánArgentinaAsociación de Biología de Tucumá