Inter-relação entre a doença periodontal e a doença de Crohn

The aim of this review was to assess the possible relationship between periodontal disease and Crohn‘s disease. The tissue destruction in periodontitis, a specific host reaction against the local accumulation of bacteria, is determined by the nature and the balance of celular and humoral immune responses. Theories have been postulated to explain the progression of gingivitis to periodontitis in the context of the Th1/Th2 paradigm. Crohn‘s disease has a Th1 profile and a number of inflammatory mediators are present in the inflamed intestinal mucosa. In conclusion, it is possible periodontitis is more prevalent in patients with Crohn‘s disease because these two diseases share pathogenic pathways, probably, due to the same T cells profile.O objetivo desta revisão foi avaliar os fatores envolvidos na possível relação entre a doença periodontal e a doença de Crohn.  A destruição tecidual na periodontite, considerada uma reação específica do hospedeiro ao acúmulo do biofilme na margem gengival, é determinada pela natureza e pelo controle das respostas imune inata e adaptativa. Teorias têm sido postuladas a fim de explicar a progressão da gengivite para a periodontite no contexto do paradigma Th1/Th2.  A doença de Crohn possui um perfil de suscetibilidade Th1 e vários mediadores inflamatórios estão induzidos na mucosa intestinal inflamada. Concluindo, é possível que haja uma maior prevalência de periodontite nos portadores de doença de Crohn, pelo fato de as duas doenças possuírem mecanismos patogênicos em comum, provavelmente, devido ao mesmo perfil linfocitário

Higher expression of Th1/Th2‐related cytokines in the intestine of Wistar rats with ligature‐induced periodontitis

Objective: This study aimed to investigate the impact of ligature-induced periodontitis (LIP) on histopathological and immunological outcomes in the colon of Wistar rats. Background: It has been repeatedly shown that inflammatory bowel disease (IBD) patients are at higher risk of developing periodontitis and presenting worse oral health than non-IBD patients. However, whether the chronic inflammatory process around teeth contributes to the pathophysiology of IBD needs to be further explored. Materials and Methods: Thirteen Wistar rats were allocated into LIP (n = 7) and controls (n = 6). Half of the colon was processed for histopathological analyses and immunohistochemical (CD45); the other half was homogenized for immunological analyses. Periodontal destruction was confirmed by measuring the distance from the cementum-enamel junction to the mandible's apical position of the mesial interproximal bone. The immunological analyses were performed with the Bio-Plex Th1/Th2 assay. Results: There was a significantly higher interproximal bone loss in LIP compared to controls. The LIP group showed a moderate infiltrate of inflammatory cells, predominantly mononucleated cells in the intestinal tissues. There was significantly higher expression of GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12 (p70), IL-13, and TNF-α in the intestinal tissues of LIP group compared to controls. Conclusion: Ligature-induced periodontitis was associated with an overexpression of Th1/Th2-related cytokines in the colon of Wistar rats

A PERIODONTITE COMO POSSÍVEL FATOR DE RISCO PARA O NASCIMENTO DE CRIANÇAS PREMATURAS DE BAIXO PESO.

O parto prematuro ocorre num período gestacional inferior a 37 semanas, uma vez que a gestação normal para humanos está em torno de 40 semanas. Crianças que ao nascimento possuem peso inferior a 2500g, são consideradas de baixo peso. A periodontite é uma alteração de origem multifatorial que acomete as estruturas que envolvem e suportam os dentes. O objetivo do trabalho foi realizar através de uma revisão de literatura uma análise sobre a importância da periodontite como fator de risco para o nascimento prematuro de crianças de baixo peso. A colonização da vagina por bactérias especificas tem sido associada com um aumento de 60% do riso de parto prematuro, e infecções no trato geniturinário tem sido associadas à complicações na gravidez durante muitos anos. Estudos recentes consideraram que mediadores inflamatórios presentes nas doenças periodontais tais como interleucina-1, fator de necrose tumoral e prostaglandina, possuem importante participação no inicio do trabalho de parto. Há evidencias que ligam a infecção materna com o parto prematuro. O presente trabalho pode concluir que a periodontite faz parte do grupo de fatores de risco de grande valia para o mecanismo de indução ao parto prematuro de crianças de baixo peso

The Resolution of Periodontal Inflammation Promotes Changes in Cytokine Expression in the Intestine and Gingival Tissues of Aged Rats with DSS-Induced Colitis

Our research aimed to explore how resolving periodontal inflammation impacts cytokine expression in the colons of aged Wistar rats. Research studies involving animals have been conducted to investigate the two-way relationship between periodontitis and inflammatory bowel disease (IBD), where chronic inflammation in either the mouth or intestines can negatively affect the other. We allocated seventeen male Wistar rats aged between 8 and 11 months to one of four groups: (1) ligature-induced periodontitis (LIP) without the resolution of periodontal inflammation (RPI) (LIP; n = 4), (2) LIP + RPI (n = 4), (3) LIP + dextran-sulphate-sodium-induced colitis (DIC) without RPI (n = 4), and LIP + DIC + RPI (n = 5). We performed histopathological and immunological analyses on periodontal and intestinal tissues and analysed cytokine expressions using a Rat Cytokine 23-Plex Immunoassay. Our findings showed that animals with and without DIC who underwent RPI showed significantly lower levels of IL-2, IL-4, IL-5, IL-10, IL-13, IL-17, IL-18, and TNF-α in the intestine compared to those without treatment. The RPI effectively reduced the number of inflammatory cells in the lamina propria and restored the epithelial barrier in the intestine in animals with DIC. The resolution of periodontal inflammation significantly reduced the levels of pro-inflammatory cytokines and chemokines in the intestines of aged rats with and without DSS-induced colitis

The international platform of registered systematic review and meta-analysis protocols (INPLASY) at 3 years: an analysis of 4,658 registered protocols on inplasy.com, platform features, and website statistics

Background: INPLASY® is an international platform for registering systematic reviews and meta-analysis protocols that was launched in March 2020. INPLASY® provides an online database in which the protocols are maintained as permanent public records and can be accessed on its website (www.inplasy.com). Methods: We described the database features and registered information of all records published since the launch of the registry on March 31, 2023. Additionally, we analyzed the website statistics dataset to explore user experience and promote data transparency. Results: Four thousand six hundred fifty-eight records were registered in INPLASY®, and more than 94% of the protocols were published within 24 h. Most of the submissions were from China, followed by Portugal, Taiwan, Malaysia, and Brazil. The INPLASY® website received 386,395 page views from 64,568 visitors during the first three years. The accesses were obtained from 170 countries. Most of the accesses were from China, followed by the US, the UK, and Portugal. The review status “completed and published” was observed in 898 protocols, and these studies were published in 372 different scientific peer-reviewed journals. The features of INPLASY® include the following: (i) INPLASY® identifier, a unique protocol number; (ii) the digital object identifier (DOI) number, the URL of the protocol linked to a specific DOI; (iii) ORCID update, INPLASY® automatically updates authors' ORCID page, including their protocol; and (iv) search tools, the protocols are freely accessible on www.inplasy.com. Conclusions: INPLASY® has several practical and useful features that should be considered when planning the registration of a systematic review protocol. Furthermore, the sharp increase in the number of protocols registered in INPLASY® in the first three years and the database statistics demonstrate that INPLASY® has become an important source of systematic review protocols. Therefore, authors should access INPLASY® before planning a future review study to avoid unintended duplication of efforts and to obtain timely registration.publishedVersio

Forward-central two-particle correlations in p-Pb collisions at root s(NN)=5.02 TeV

Two-particle angular correlations between trigger particles in the forward pseudorapidity range (2.5 2GeV/c. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B. V.Peer reviewe

Event-shape engineering for inclusive spectra and elliptic flow in Pb-Pb collisions at root(NN)-N-S=2.76 TeV

Peer reviewe

Production of He-4 and (4) in Pb-Pb collisions at root(NN)-N-S=2.76 TeV at the LHC

Results on the production of He-4 and (4) nuclei in Pb-Pb collisions at root(NN)-N-S = 2.76 TeV in the rapidity range vertical bar y vertical bar <1, using the ALICE detector, are presented in this paper. The rapidity densities corresponding to 0-10% central events are found to be dN/dy4(He) = (0.8 +/- 0.4 (stat) +/- 0.3 (syst)) x 10(-6) and dN/dy4 = (1.1 +/- 0.4 (stat) +/- 0.2 (syst)) x 10(-6), respectively. This is in agreement with the statistical thermal model expectation assuming the same chemical freeze-out temperature (T-chem = 156 MeV) as for light hadrons. The measured ratio of (4)/He-4 is 1.4 +/- 0.8 (stat) +/- 0.5 (syst). (C) 2018 Published by Elsevier B.V.Peer reviewe