Réalité virtuelle et augmentée, un phénomène grandissant: meilleures utilisations touristiques internationales et recommandations pour le tourisme de montagne valaisan

Le sujet de cette thèse de Bachelor porte sur la réalité augmentée et le tourisme. Dans un premier temps, la réalité augmentée et son fonctionnement sont expliqués afin de mieux comprendre cette technologie. Ensuite, des applications de réalité augmentée dans le tourisme international sont détaillées et des critères de succès sont établis afin de pouvoir définir les meilleures utilisations de réalité augmentée dans le tourisme international. Puis, les actuels enjeux et défis de la réalité augmentée sont exposés et les évolutions futures probables de la réalité augmentée sont parcourues. Enfin, la dernière partie est plus ciblée et créative car elle est consacrée à des recommandations de réalité augmentée pour le tourisme Valaisan. Au sujet de la méthodologie, la première partie, étant surtout technique, est basée majoritairement sur des sources secondaires (ouvrages ou articles scientifiques). La seconde partie s’appuie principalement sur des ressources venant d’Internet. La troisième partie comprend des réflexions personnelles tout en étant aussi fondée sur des ouvrages scientifiques. Quant à la dernière partie, afin de pouvoir formuler des idées pertinentes, une interview d’expert et un questionnaire en ligne destiné aux offices du tourisme valaisan ont été réalisés. Il s’agit ensuite d’idées et de réflexions personnelles pour les recommandations de projets pour le tourisme valaisan

Neurons as a model system for cryo-electron tomography.

Cryo-electron tomography (Cryo-ET) provides unique opportunities to image cellular components at high resolution in their native state and environment. While many different cell types were investigated by cryo-ET, here we review application to neurons. We show that neurons are a versatile system that can be used to investigate general cellular components such as the cytoskeleton and membrane-bound organelles, in addition to neuron-specific processes such as synaptic transmission. Furthermore, the synapse provides a rich environment for the development of cryo-ET image processing tools suitable to elucidate the functional and spatial organization of compositionally and morphologically heterogeneous macromolecular complexes involved in biochemical signaling cascades, within their native, crowded cellular environments

Analyse d’un gourmet bag dans la restauration valaisanne et conditions pour sa mise en place

L’objectif de ce travail est de mener une étude sur le gourmet bag et sa possible introduction dans la restauration valaisanne. Cet emballage, offrant la possibilité aux clients d’emporter leurs restes de nourriture, a pour objectif de prévenir le gaspillage alimentaire et d’apporter divers bénéfices aux restaurateurs et aux consommateurs. En plus d’être un service supplémentaire au client, le gourmet bag peut endosser d’autres rôles, et notamment celui d’être un support de communication original pour une entreprise ou une destination touristique

Elaboration d’une stratégie marketing afin d’augmenter l’attractivité touristique de Vercorin

L’objectif principal de ce travail est d’apporter des solutions et d’émettre des recommandations aux acteurs touristiques de Vercorin dans le but d’augmenter l’attractivité touristique de la destination

Tetraphenylethylene–DNA conjugates: influence of sticky ends and DNA sequence length on the supramolecular assembly of AIE-active vesicles

The supramolecular assembly of DNA conjugates, functionalized with tetraphenylethylene (TPE) sticky ends, into vesicular structures is described. The aggregation-induced emission (AIE) active TPE units allow to monitor the assembly process by fluorescence spectroscopy. The number of TPE modifications in the overhangs of the conjugates influences the supramolecular assembly behavior. A minimum of two TPE residues on each end are required to ensure a well-defined assembly process. The design of the presented DNA-based nanostructures offers tailored functionalization with applications in DNA nanotechnology

Supramolecular assembly of pyrene-DNA conjugates: influence of pyrene substitution pattern and implications for artificial LHCs.

The supramolecular self-assembly of pyrene-DNA conjugates into nanostructures is presented. DNA functionalized with different types of pyrene isomers at the 3'-end self-assemble into nano-objects. The shape of the nanostructures is influenced by the type of pyrene isomer appended to the DNA. Multilamellar vesicles are observed with the 1,6- and 1,8-isomers, whereas conjugates of the 2,7-isomer exclusively assemble into spherical nanoparticles. Self-assembled nano-spheres obtained with the 2,7-dialkynyl pyrene isomer were used for the construction of an artificial light-harvesting complex (LHC) in combination with Cy3 as the energy acceptor

Supramolecular Assembly of Pyrene-DNA Conjugates into Columnar Vesicles

This poster describes the supramolecular assembly of DNA conjugates functionalized with pyrene sticky-ends. After hybridization, the 3’-end modified DNA single strands self-assembled into vesicles with diameters of 50–200 nm. Columnar packed aggregated and multilamellar vesicles were observed by cryo-EM

A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics

Ribosome-associated non-coding RNAs (rancRNAs) have been recognized as an emerging class of regulatory molecules capable of fine-tuning translation in all domains of life. RancRNAs are ideally suited for allowing a swift response to changing environments and are therefore considered pivotal during the first wave of stress adaptation. Previously, we identified an mRNA-derived 18 nucleotides long rancRNA (rancRNA_18) in Saccharomyces cerevisiae that rapidly downregulates protein synthesis during hyperosmotic stress. However, the molecular mechanism of action remained enigmatic. Here, we combine biochemical, genetic, transcriptome-wide and structural evidence, thus revealing rancRNA_18 as global translation inhibitor by targeting the E-site region of the large ribosomal subunit. Ribosomes carrying rancRNA_18 possess decreased affinity for A-site tRNA and impaired structural dynamics. Cumulatively, these discoveries reveal the mode of action of a rancRNA involved in modulating protein biosynthesis at a thus far unequalled precision

Characterization of two novel proteins involved in mitochondrial DNA anchoring in Trypanosoma brucei.

Trypanosoma brucei is a single celled eukaryotic parasite in the group of the Kinetoplastea. The parasite harbors a single mitochondrion with a singular mitochondrial genome that is known as the kinetoplast DNA (kDNA). The kDNA consists of a unique network of thousands of interlocked circular DNA molecules. To ensure proper inheritance of the kDNA to the daughter cells, the genome is physically linked to the basal body, the master organizer of the cell cycle in trypanosomes. The connection that spans, cytoplasm, mitochondrial membranes and the mitochondrial matrix is mediated by the Tripartite Attachment Complex (TAC). Using a combination of proteomics and RNAi we test the current model of hierarchical TAC assembly and identify TbmtHMG44 and TbKAP68 as novel candidates of a complex that connects the TAC to the kDNA. Depletion of TbmtHMG44 or TbKAP68 each leads to a strong kDNA loss but not missegregation phenotype as previously defined for TAC components. We demonstrate that the proteins rely on both the TAC and the kDNA for stable localization to the interface between these two structures. In vitro experiments suggest a direct interaction between TbmtHMG44 and TbKAP68 and that recombinant TbKAP68 is a DNA binding protein. We thus propose that TbmtHMG44 and TbKAP68 are part of a distinct complex connecting the kDNA to the TAC

Asymptotics of unitary and othogonal matrix integrals

In this paper, we prove that in small parameter regions, arbitrary unitary matrix integrals converge in the large $N$ limit and match their formal expansion. Secondly we give a combinatorial model for our matrix integral asymptotics and investigate examples related to free probability and the HCIZ integral. Our convergence result also leads us to new results of smoothness of microstates. We finally generalize our approach to integrals over the othogonal group.Comment: 41 pages, important modifications, new section about orthogonal integral