Electrochemical Methods in Clinical Immunoassays and Nucleic Acid Analyses

文介绍电化学方法在免疫检测及核酸分析中的应用. 鉴于有关方面的文献综述已有多篇，这里着重于已经商品化和临床应用的电化学分析技术. 希望从这些成功地应用于体外诊断的方法中得到一些启示，并提出作者个人对于该领域研究前景的看法.The applications of electrochemical methods in immunoassays and nucleic acid analyses are discussed. Instead of a comprehensive literature review, this paper focuses on the techniques that have already been commercialized and used in clinical diagnoses. Learning from the success of these methods will help us to further understand the strengths and limitations of electroanalytical technology. A personal perspective of the research and development in these fields has been presented.作者联系地址：新加坡科技研究局生物工程与纳米科技研究院，新加坡 138669Author's Address: Institute of Bioengineering and Nanotechnology, A*STAR, Singapore 13866

Study on the Hydrophilicity of Nafion Membrane Surface

Ｎａｆｉｏｎ膜在电化学体系中的应用越来越广泛，而其表面亲水性往往直接影响着使用性能．本文介绍一种适宜于表征Ｎａｆｉｏｎ膜表面亲水性的接触角测量方法，即束缚气泡法，并讨论了该膜与水溶液接触角的滞后现象、溶液表面张力的影响，简介了用全氟表面活性剂改善Ｎａｆｉｏｎ膜表面亲水性的尝试．Due to its high chemical stability and ion selective permeability, Nafion membrane has been applied in many electrochemical systems. The surface hydrophilicity is one of the main factors affecting its performance. In this paper, the bound bubble method is introduced as a suitable way to measure the contact angles between Nafion membrane and various aqueous solutions. During the measurement, Nafion membrane is saturated with given electrolyt solution. The hysteresis of the contact angles observed in the measurement is attributed to the co existance of hydrophobic and hydrophilic domains at the membrane surface. The effect of the solution surface tension on the contact angle is demonstrated. It is shown that Nafion membrane can be rendered more hydrophilic by modification with sulfonic acid type perfluorinated surfactant.作者联系地址：武汉大学化学系Author's Address: Dept. of chem., Wuhan Univ., Wuhan 43007