14 research outputs found
Analyses phytochimiques et propriĂ©tĂ©s des composĂ©s bioactifs: amĂ©lioration dâune huile vĂ©gĂ©tale par incorporation des extraits de plantes steppiques
La multitude de structures phĂ©noliques qui pourraient ĂȘtre contenus dans un matĂ©riel vĂ©gĂ©tal donnĂ©, ainsi que leur teneur
élevée en polyphénols totaux, permet leur utilisation en tant que facteurs antioxydants efficaces pour les corps gras. Le but
de cette recherche est d'évaluer l'efficacité des extraits de deux plantes médicinales préparés à partir de trois solvants
diffĂ©rents (EtOH, EthyacĂ©tate et lâeau) dans une huile d'olive algĂ©rienne pauvre en antioxydants naturels exposĂ©e Ă une
oxydation forcée au rancimat. Les extraits ont été ajoutés à 300, 500 et 900 ppm de niveau d'équivalence phénolique.
AprĂšs oxydation on rĂ©Ă©value les paramĂštres physico-chimiques pour suivre la dĂ©gradation des Ă©chantillons dâhuiles
sujettes Ă la thermo-oxydation. Tandis quâune Ă©tape primordiale du dosage des antioxydants tant quâune Ă©valuation de leur
potentiel antioxydant a également été réalisés comme premiÚre étape dans les extraits de plantes et de l'huile avant le
chauffage, et seulement sur l'huile aprĂšs le chauffage pour Ă©valuer les la perte en antioxydants intervenant dans le
processus antioxydant. Ainsi une teneur élevée en polyphénols totaux (en mg eq AG/g MS) et flavonoïdes (en mg eq
CAT/gMS) a Ă©tait respectivement notĂ©e pour lâextrait EtOH/H2O (456,05±8,54, 30,21±0,28) suivi de lâextrait aqueux
(424,23±7,79, 46,45±0,11) et lâEthylacĂ©tate (229,76±4,76, 9,13±0,19). Ce qui permet tout de mĂȘme Ă lâextrait EtOH/H2O
dâavoir le potentiel antioxydant le plus remarquable (IC50= 88,687ÎŒg/ml). Les rĂ©sultats du Rancimat conduisent Ă
conclure quâune concentration moyenne en extrait pourra avoir des temps dâinduction les plus Ă©levĂ©s (15h +/- 0,5). Sur la
base des analyses statistiques obtenus, il est irrĂ©futable que l'utilisation dâadditifs naturels d'origine vĂ©gĂ©tale pour
l'enrichissement de lipides (graisses et huiles) et les aliments contenant des lipides est une perspective a considéré afin de
prolonger la durée de vie des matiÚres grasses.info:eu-repo/semantics/publishedVersio
Bioactive properties of medicinal plants from the Algerian flora: selecting the species with the highest potential in view of application purposes
The Algerian flora contains a wide variety of plant species with potential to be used in medicinal applications. Herein, the bioactive properties of medicinal plants from Algeria were evaluated to select the species with highest suitability to be used under specific purposes, while scientifically validating their health claims. The antioxidant activity of the infusions was screened by using several tests and cytotoxic properties were evaluated against human tumor cell lines (as also against non-tumor cells). Different hydrophilic bioactive compounds were also quantified. The results were analyzed considering individual variations in each parameter (ANOVA), but also in an aggregated approach by applying principal component analysis to acquire a comprehensive knowledge regarding the overall bioactive potential of the studied species. Limoniastrum guyonianum and Thymus pallescens showed the highest antioxidant activity (EC50 values ranging from 29 to 229 ÎŒg/mL and 54 to 240 ÎŒg/mL, respectively), whilst Asteriscus graveolens and L. guyonianum gave the best cytotoxicity against human tumor cell lines (GI50 values ranging from 11 to 29 ÎŒg/mL and 22 to 70 ÎŒg/mL, respectively). T. pallescens stood out as the species with highest bioactive compounds contents (phenols: 463 mg GAE, flavonoids: 194 mg CE, esters; 186 mg CAE; flavonols: 85 mg QE, considering g of lyophilized infusion basis). From a global point of view, T. pallescens, Saccocalyx satureioides and Ptychotis verticillata proved to be the preferable choices as high potential sources of bioactive compounds, while Haloxylon scoparium, L. guyonianum and A. graveolens would be the most suitable matrices considering the bioactivity (especially cytotoxicity) criterion, as inferred from the PCA outputs.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support to CIMO (PEst-OE/AGR/UI0690/2014), R.C. Calhelha (SFRH/BPD/68344/2010), J.C.M. Barreira (SFRH/BPD/72802/2010) and L. Barros (âCompromisso para a CiĂȘncia 2008â contract)
Phenolic compounds characterization by LC-DAD- ESI/MSn and bioactive properties of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne
Scientific research has been focused on finding natural occurring molecules from plant origin. Herein, infusion,
decoction and hydroethanolic extracts of Thymus algeriensis Boiss. & Reut. and Ephedra alata Decne. from Algeria
were phytochemically characterized by LC-DAD-ESI-MSn, and evaluated regarding bioactive properties (antioxidant
and antibacterial). Flavonol and flavone glycoside derivatives and phenolic acids, specially rosmarinic
acid and kaempferol-O-glucuronide were the major compounds in T. algeriensis extracts. Otherwise, E. alata
presented isoflavones and flavonol derivatives as main compounds, being hydroxypuerarin isomer 1 the major
molecule. Aqueous extracts had significantly higher antioxidant activity, being this activity correlated with the
amount of phenolic compounds. Antimicrobial activity of the extracts was tested against multi-resistant bacteria
strains from clinical isolates. The obtained MIC values indicate that the hydroethanolic extracts revealed the
highest effect, especially the one of T. algeriensis against Gram-positive bacteria. Hence, these plant extracts
could be used in the development of bioactive ingredients.The authors are grateful to the Foundation for Science and
Technology (FCT, Portugal) and FEDER under Programme PT2020 for
financial support to CIMO (UID/AGR/00690/2013), S. Heleno grant
(SFRH/BPD/101413/2014) and L. Barros contract. The authors are also
grateful to the Interreg España-Portugal for financial support through
the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
Infusions obtained from two medicinal plants as a source of polyphenols with antibacterial activity
Several researches are now oriented towards studying natural molecules of plant origin. In Algeria, many
herbs are used as traditional remedies to treat different diseases. In this context, the phenolic compounds of
the aqueous extracts, obtained by infusion, of two plant species, Saccocalyx satureioides Coss.& Dur and
Limoniastrum guyonianum Boiss., from the Algerian flora, were identified and quantified by HPLC-DADESI/
MS, and their antibacterial activity was evaluated against clinical isolates. The results indicate the
presence of several phenolic acids, in particular, derivatives of caffeic acid with glycosylated flavonoids.
While for S. satureioides 13 phenolic compounds were identified and the rosmarinic acid was the most
abundant phenolic acid, L. guyonianum showed a high abundance of myricetin and derivatives such as
myricetin-3-O-glucoside and myricetin-O-rhamnoside among the eight molecules detected. The antibacterial
activity of the infusions was significant with MIC values between 2.5 and 20 mg/mL, however, L.
guyonianum had the highest activity on all the tested bacteria. Staphylococcus aureus and Pseudomonas
aeruginosa were the most sensitive and the most resistant strains, respectively. Consequently, the studied
plant species are sources of natural antibacterial substances that can be used to fight against pathogenic
microorganisms.info:eu-repo/semantics/publishedVersio
Profiling polyphenol composition by HPLC-DAD-ESI/MSn and the antibacterial activity of infusion preparations obtained from four medicinal plants
The infusions of Thymus pallescens Noë, Saccocalyx satureioides Coss. et Dur., Ptychotis verticillata Briq.
and Limoniastrum guyonianum Boiss. have been used as medicinal remedies for many diseases in Algerian
folk medicine. These species have also been well documented as rich sources of phytochemicals, such as
phenolic compounds with wide diversified chemical structures, which exhibit far-ranging biological activities.
Thus, the phenolic compound profile of the aqueous extracts, obtained by infusing, of the mentioned
species was obtained by HPLC-DAD-ESI/MS, and their antibacterial activity was evaluated against clinical
isolates. Several phenolic acids were identified and quantified, particularly caffeic acid derivatives along with
glycosylated flavonoids. T. pallescens and S. satureioides contain 13 phenolic compounds, where rosmarinic
acid was the most abundant phenolic acid present, while L. guyonianum presented myricetin-3-O-glucoside
and myricetin-O-rhamnoside as the main compounds among the eight detected molecules.
P. verticillata presented a profile of ten phenolic compounds, where 5-O-caffeoylquinic acid was the most
abundant phenolic acid, followed by the flavone luteolin-3-O-glucoside. The antibacterial activity of the
infusions ranged between 2.5 and 20 mg mLâ1 (MIC values), and L. guyonianum showed the highest activity
against all of the tested bacteria, Staphylococcus aureus and Pseudomonas aeruginosa being the most
sensitive and resistant strains, respectively. Thus, the studied plant species are sources of natural antibacterial
substances that can be used to fight against pathogenic microorganisms.The authors are grateful to the Foundation for Science and
Technology (FCT, Portugal) and FEDER under Programme
PT2020 for financial support to CIMO (UID/AGR/00690/2013),
S. Heleno (SFRH/BPD/101413/2014) grant and L. Barros contract.
The authors are also grateful to the Interreg España-
Portugal for financial support through the project
0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
Actividad antimicrobiana de Acacia tortilis en patĂłgenos comensales y microbiota vaginal no patĂłgena
Enquadramento: a vaginose Bacteriana (VB) é a doença infeciosa mais
comum do trato vaginal, afeta muitas mulheres em todo o mundo e
ocorre quando hĂĄ um desequilĂbrio na microbiota vaginal, ocorrendo
diminuição de Lactobacillus 1-4. A Acacia tortilis é conhecida pelas suas
propriedades medicinais de entre as quais se destacam a sua atividade
antibacteriana e antifĂșngica.
Objetivo: avaliar a atividade antimicrobiana do extrato hidroetanĂłlico de
Acacia tortilis em patogénicos vaginais e em microbiota vaginal humana.
Metodologia: estudo experimental. O extrato hidroetanĂłlico de A. tortilis foi
obtido por um método de maceração com agitação utilizando etanol/
ĂĄgua (80:20 v/v) Ă temperatura ambiente. A atividade antimicrobiana foi
avaliada pelo método da microdiluição e ensaio colorimétrico com
cloreto de p-iodonitrotetrazĂłlio (INT).
Resultados: o extrato hidroetanólico apresentou valores de concentraçÔes
mĂnimas inibitĂłrias (CMIs) bastante promissoras em microrganismos
patogénicos como Escherichia coli, Proteus mirabilis e Gardenerella
vaginalis (1,25 mg/mL). JĂĄ para Neisseria gonorrhoeae, a CMI aumentou
para 5 mg/mL. Relativamente à microbiota vaginal não-patogénica, os
trĂȘs Lactobacillus testados apresentaram CMIs de 5 mg/mL. Comparando
as CMIs dos patogénicos comensais com a microbiota vaginal não
patogénica comensal, verificamos que para uma concentração de 2,5
mg/mL de extrato, foram inibidos alguns patogénicos comensais como
Gardenerella vaginalis, Proteus mirabilis e Escherichia coli, no entanto, os
Lactobacillus (casei, plantarum , delbrueckii subs. bulgaricus LMG) testados
não sofreram inibição.
ConclusĂŁo: o extrato hidroetanĂłlico de Acacia tortilis inibiu o crescimento
de alguns patogénicos comensais sem afetar o crescimento da
microbiota vaginal não patogénica (Lactobacillus).info:eu-repo/semantics/publishedVersio
Phenolic profiling, biological activities and in silico studies of Acacia tortilis (Forssk.) Hayne ssp. raddiana extracts
The authors are grateful to the Foundation for Science and
Technology (FCT, Portugal) for financial support through national
funds FCT/MCTES to CIMO (UIDB/00690/2020). L. Barros and R. C.
Calhelha thank the national funding by the FCT, P.I., through the institutional
scientific employment program-contract for their contracts.
M. Carocho also thanks the project ValorNatural for his research contract.
The authors are also grateful to the FEDER-Interreg España-
Portugal programme for financial support through the project
0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
HPLC-DAD-ESI-MS/MS screening of phytochemical compounds and the bioactive properties of different plant parts of: Zizyphus lotus (L.) Desf.
Zizyphus lotus L. (Desf.) has been widely used as a homemade ingredient to treat numerous diseases in Algerian folk medicine. In this context, decoction, infusion and hydroethanolic extracts were prepared from the branches, leaves, roots, and stem barks. The extracts were characterized regarding their phytochemical composition by HPLC-DAD-ESI/MSn and were assessed for in vitro bioactivity (cytotoxicity, anti-inflammatory activity, and antibacterial activity). A total of 29 compounds (flavonoid derivatives and secoiridoids) were identified in Z. lotus, 15 being detected in leaves, 10 in branches, 5 in roots and 7 in stem barks. The highest contents of these compounds, especially secoiridoids, were detected in branches followed by leaves, oleuropein being the main compound detected in leaves and oleoside in branches. The aqueous preparations of the leaves and root barks revealed the highest cytotoxicity and anti-inflammatory potential. On the other hand, as for the antibacterial activity, the hydroethanolic extract of the branches showed the highest potential, especially against the MSSA strain. Hence, the chemical diversity found in Z. lotus makes it a source of bioactive ingredients that can be applied in the formulations of different ingredients in the food and pharmaceutical industries.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Program PT2020 for financial support to CIMO (UID/AGR/00690/2019); to the national funding by FCT, P. I., through the institutional scientific employment program-contract for L. Barros and R. Calhelha and through the individual scientific employment program-contract for S. A. Heleno; to the University of Mostaganem and MESRS of Algeria for the financial support that allowed to achieve this work; and also to the Interreg España-Portugal for financial support through the project 0377_Iberphenol_6_E.info:eu-repo/semantics/publishedVersio
Food preservative extracts from pumpkin by-products
Pumpkin is a vegetable widely consumed around the world, popularly known for its nutritional
and pharmacological properties. Some reports in the literature reveal the rich profile of nutrients
and bioactive compounds of pumpkin varieties [1,2]. However, the seeds, peels, and fibers
resulting from pumpkin processing are still poorly explored by food industry. Considering the
alarming scenario of losses and waste during all the food supply chain worldwide [3] and
seeking to promote a more sustainable food system, the present study purposed to investigate
the by-products of different varieties of pumpkin as a source of extracts with preservative
capacity for food application. For that purpose, hydroethanolic extracts from the varieties
âButternut squashâ, âCommon pumpkinâ, and âKabocha squashâ from Portugal, and âButternut
squashâ, âCommon pumpkinâ, and âMusquĂ©e de provenceâ from Algeria, were evaluated in
terms of their bioactivities, more specifically the antioxidant, antimicrobial, and cytotoxic
capacities. All the samples presented great antioxidant capacity through two based-cell assays,
namely the lipid peroxidation inhibition capacity (TBARS) and the anti-hemolytic activity
(OxHLIA). Interestingly, the seeds of the common pumpkin from Algeria did not present antihemolytic
properties, despite showing the strongest lipid peroxidation inhibition capacity. In
fact, the seeds stood out for all the pumpkin varieties in the TBARS assay, while in the OxHLIA
assay, the results were quite similar between the type of bioresidues and between the varieties.
Moreover, the samples from Portugal showed greater antioxidant capacity than the Algerian
ones. Regarding cytotoxicity, the effect of inhibiting non-tumor cell growth was not observed,
even at the highest tested concentration of 400 ÎŒg/mL. This first validation is of great
importance to prevent food safety issues. These preliminary results are the basis for future
studies aiming at the valorization of these bioresidues from food industry as a great source of
preservative compounds toward the replacement of synthetic additives with natural alternatives
allied to health benefits, as also the promotion of a circular economyThe authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support
through national funds FCT/MCTES to CIMO (UIDB/00690/2020); national funding by FCT, P.I., through the
institutional scientific employment program-contract for C. Pereira and L. Barros contracts and A.K. Molina and
M.G. Leichtweis PhD grants (2020.06231.BD and 2020.06706.BD, respectively). To FCT, P.I., within the scope
of the Project PRIMA Section 2 - Multi-topic 2019: PulpIng (PRIMA/0007/2019).info:eu-repo/semantics/publishedVersio
Hemi-synthesis of novel (S)-carvone hydrazone from Carum carvi L. essential oils: structural and crystal characterization, targeted bioassays and molecular docking on human protein kinase (CK2) and Epidermal Growth factor Kinase (EGFK)
Polyfunctional N,O,O,N-type ligands such as the oxalyl dihydrazide (ODH) may induce formation of mono- , di-, and polynuclear complexes with natural monoterpene ketones, involving ligand bridging and Oxo- bridging. In this context, a novel chiral dihydrazone is designed through hemi-synthesis process by re- acting oxalyldihydrazide (ODH) with ( s )-carvone, the major compound of carawayâs seeds essential oil. The C = N imine bi-condensation is performed without prior isolation of the natural ( s )-carvone and the resulting ( s )-carvone dihydrazone (s-CHD) is structurally characterized by Single-crystal X-ray diffrac- tion, 2D-NMR spectroscopy and chiral LCMS analysis to confirm the formation of a single pure enan- tiomer. In -vitro cell-based assays were conducted on normal fibroblast (L929) using a presBlue (PB) flu- orescence quantification method of cell-viability and by sulforhodamine B calorimetric cytotoxicity as- says to determine the anti-proliferative effect on four human tumoral lines (NCI-H460, Hela, HepG2 and MCF-7) and normal PLP2. Anti-inflammatory assays were determined through NO production by Maurine LPS-stimulated macrophages (RAW 264.7). The ( s )-CHD has no effect on normal cells viability ( > 88%) and PLP2 (GI50 = 326 ug/mL), while a moderate ( âŒ55%) to significant ( âŒ63%) antigrowth potential was recorded against HepG2, Hela and MCF-7 tumor cell lines, where RAW 264.7 was feebly sensitive. A molecular docking was performed using Autodock vina software on the protein kinase CK2 and Epi- dermal Growth factor Kinase proteins EGFK and the dock scores allowed to identify significant bind- ing affinities (lower G and Ki values) and potential hydrophilic/hydrophobic interactions with ( s )-CHD comparing to the clinical ellipticine as potential ligands. Molecular docking suggests that ( s )-CHD pos- sesses high affinity towards the kinase domain receptors CK2 and EGFR, being able to bind to the ATP region.Thanks are due to the Research Center Scientific and Technical in Analyzes Physico-Chimiques CRAPC Algerian Directorate for research DGRSDT for the financial support. The authors thanks Fundação para a CiĂȘncia e a Tecnologia (FC&T, Lisbon) for financial support through projects PTDC/MECâONC/29327/2017 and PTDC/EQU-EQU/32473/2017. We are thankful to NOVA University of Lisbon (FCT/UNL) for the financial support from Erasmus + EU international credit mobility 2017â2019. the laboratory for Green Chemistry LAQV-REQUIMTE FCT/MCTES (UID/QUI/50006/2019) is co-financed by the ERDF and the chemistry department for providing the instruments support.info:eu-repo/semantics/publishedVersio