Development Of Identification Technique By FTIR-PCA For Supercritically Extracted Metabolites From Parkia Speciosa (Hassk) Seeds [TP156.E8 S171 2007 f rb].

Analisis kualitatif dan kuantitatif terhadap hasil ekstrak bendalir lampau genting karbon dioksida (BLG-CO2) telah dilakukan pada beberapa keadaan pengekstrakan. The qualitative and quantitative analysis on the yield of Parkia speciosa seeds extracted by Supercritical Carbon Dioxide Extraction (SC-CO2 extraction) were performed under various extraction conditions

Development Of Identification Technique By Ftir-Pca For Supercritically Extracted Metabolites From Parkia Speciosa (Hassk) Seeds

Analisis kualitatif dan kuantitatif terhadap hasil ekstrak bendalir lampau genting karbon dioksida (BLG-CO2) telah dilakukan pada beberapa keadaan pengekstrakan. The qualitative and quantitative analysis on the yield of Parkia speciosa seeds extracted by Supercritical Carbon Dioxide Extraction (SC-CO2 extraction) were performed under various extraction conditions

Development of Polymeric Nanoparticles of Garcinia mangostana Xanthones in Eudragit RL100/RS100 for Anti-Colon Cancer Drug Delivery

Xanthones are a group of oxygenated heterocyclic compounds with anticancer properties, but poor aqueous solubility and low oral bioavailability hinder their therapeutic application. This study sought to prepare a xanthones extract (81

Profile of Parkia speciosa hassk metabolites extracted with SFE using FTIR- PCA method

A rapid identification, classification and discrimination tool, using Fourier Transform Infrared (FTIR) spectroscopy combined with Principal Component Analysis (PCA), was developed and applied to determine the profile of the Supercritical Fluid Extraction (SFE) of Parkia speciosa seeds under various temperature and pressure conditions (313, 323, 333, 343, 353 and 363 K and 20.68, 27.58, 34.47, 41.37, 48.26, and 55.16MPa). The separation and identification of the compounds was carried out by Gas Chromatography coupled with Time of Flight Mass Spectrometry (GC/TOF-MS). This technique has made it possible to detect the variability obtained under different SFE conditions and the separation of different chemical compounds in P. speciosa seeds. The FTIR-PCA results were verified by GC/TOF-MS, and the FTIR-PCA method successfully identified the unsaturated carboxylic acids with the highest percentage area under the different conditions

Antiangiogenic properties of Koetjapic acid, a natural triterpene isolated from Sandoricum koetjaoe Merr

Background: Angiogenesis, the formation of new blood vessels, has become an important target in cancer therapy. Angiogenesis plays an important role in tumor growth and metastasis. Koetjapic acid (KA) is a seco-A-ring oleanene triterpene isolated from S. koetjape. The solvent extract of this plant species was shown previously to have strong antiangiogenic activity; however the active ingredient(s) that conferred the biological activity and the mode of action was not established. Given the high concentration of KA in S. koetjape, an attempt has been made in this study to investigate the antiangiogenic properties of KA.Results: Treatment with 10-50 μg/ml KA resulted in dose dependent inhibition of new blood vessels growth in ex vivo rat aortic ring assay. KA was found to be non-cytotoxic against HUVECs with IC40.97 ± 0.37 μg/ml. KA inhibited major angiogenesis process steps, endothelial cell migration and differentiation as well as VEGF expression.Conclusions: The non-cytotoxic compound, KA, may be a potent antiangiogenic agent; its activity may be attributed to inhibition of endothelial cells migration and differentiation as well VEGF suppression

Extraction and identification of vitamin E from Pithecellobium Jiringan seeds using supercritical carbon dioxide

Pithecellobium Jiringan (P. Jiringan) is traditionally known as natural herb consists of several medicinal compounds (vitamin E). Supercritical carbon dioxide extraction (SC-CO2) has been proven as potential method to extract interest compound from herbs. By altering pressure and temperature, the specific compound can be extracted. In this study, the SC-CO2 operating conditions are pressure (20.68 MPa to 55.16 MPa) and temperature (40°C to 80°C) in one hour extraction regime was used to extract vitamin E from P. jiringan. The quantification of vitamin E was analysed with Gas Chromatography Time of Flight Mass Spectrometry (GC-TOF-MS). The responses are overall oil yield and vitamin E yield. The overall oil yield was obtained at the highest condition of 55.16 MPa and 80°C with asymptotic yield of 8.06%. In contrast, the highest amount of vitamin E obtained is 0.0458mg/g sample (80.14 ppm) at the lowest extraction condition of 20.68 MPa and 40ᵒ

The effects of solvents and extraction methods on the antioxidant activity of P. niruri

The effects of different types of solvents and extraction method were investigated to determine the presence of antioxidant contents and activity from the P. niruri plant. The aim of this study is to determine which extraction method will give higher natural antioxidant contents and antioxidant activity. The content of natural antioxidant and antioxidant activity were analysed by total phenolic content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The results showed that extracts from a supercritical fluid extraction (SFE) method without the addition of modifier showed the highest content of total phenolic (187.66 mg GAE/ g) and flavonoid (1100.93 mg QE/g) in P. niruri compared to the other methods of extraction with different type of solvents. The extract of P. niruri from different extraction methods showed antioxidant activity on DPPH radical scavenging assay. The soxhlet extraction method by methanol showed the lowest IC50 compared to the other methods of extraction. The results revealed that P. niruri extracts had different content of antioxidant and antioxidant activity. The solvent polarity and different methods of extraction play significant roles in determining the most suitable method for production of antioxidant contents and antioxidant activity from P. niruri extracts

Profile of parkia speciosa hassk metabolites extracted with sfe using FTIR- PCA method

A rapid identification, classification and discrimination tool, using Fourier Transform Infrared (FTIR) spectroscopy combined with Principal Component Analysis (PCA), was developed and applied to determine the profile of the Supercritical Fluid Extraction (SFE) of Parkia speciosa seeds under various temperature and pressure conditions (313, 323, 333, 343, 353 and 363 K and 20.68, 27.58, 34.47, 41.37, 48.26, and 55.16 MPa). The separation and identification of the compounds was carried out by Gas Chromatography coupled with Time of Flight Mass Spectrometry (GC-TOF‐MS). This technique has made it possible to detect the variability obtained under different SFE conditions and the separation of different chemical compounds in P. speciosa seeds. The FTIR‐PCA results were verified by GC-TOF‐MS, and the FTIR‐PCA method successfully identified the unsaturated carboxylic acids with the highest percentage area under the different conditions

Efficacy of biological activity of andrographis paniculata extracted by using supercritical Carbon dioxide (SC-CO2) extraction

Andrographis paniculata plant has been widely used for traditional medicine such as curing fever, diarrhoea, and inflammation. In this study, the main component (Andrographolide) is extracted from the leaves of A. paniculata using supercritical carbon dioxide (Sc-CO2) and Sc-CO2 assisted by methanol as a modifier solvent. The methanol soxhlet extraction as a standard method was used to compare with the Sc-CO2 extraction. This work also focuses on the determination of anti-oxidant activity of Sc-CO2 extraction extracts of A. paniculata plant. The Sc-CO2 extracts of A. paniculata for both Sc-CO2 extractions with and without modifier showed promising antioxidant activity. Free radical scavenging potential of both Sc-CO2 extractions of A. paniculata plant was evaluated by using reducing power. In this method, ascorbic acid was used as a standard for determining reducing power. Both Sc-CO2 extract of A. paniculata plant exhibited appreciable activity as compared to the methanol soxhlet extract, indicating that A. paniculata has promising free radical scavenging activity