ORY-1001 Suppresses Cell Growth and Induces Apoptosis in Lung Cancer Through Triggering HK2 Mediated Warburg Effect

ORY-1001, an inhibitor of covalent lysine (K)-specific demethylase 1A (KDM1A), has been used as a therapy for the treatment of acute leukemia. However, the underlying mechanisms of anticancer are still not fully elucidated. Here, we report that KDM1A is highly expressed in lung cancers, where it appears to drive aggressive growth. Furthermore, lung cancer patients with higher KDM1A levels have worse survival outcomes than patients with lower KDM1A levels. Interestingly, ORY-1001significantly inhibited the cell proliferation, colony formation, cell cycle, and induced apoptosis, by regulating the Warburg effect through controlling Hexokinases 2 (HK2) expression. In summary, these results indicate that ORY-1001 could inhibit the growth of lung cancer cells via regulating the Warburg effect by controlling HK2

Increase of available soil silicon by Si-rich manure for sustainable rice production

Depletion of bioavailable silicon, Si, in paddy soils can decrease the yields of rice. A potential solution is to amend soil with Si-rich organic wastes such as manure from animals fed with rice crop residues. Here, we studied Si in soils from 2000 to 2010 field experiments without manure, with 5 and 10 years of manure, in Eastern China. Results showed that available Si in soils increased from 130 to 270 mg kg-1 after 10 years of manure amendment. This finding is explained either by direct input of available Si or by Si produced by mineralization of Si minerals. To conclude, our results show that amending soil with Si-rich manure in the long term is a solution for sustainable rice production.</p

Radiofrequency ablation of metastatic chondrosarcoma-associated refractory ventricular tachycardia originating from the right ventricular outflow tract: A case report and literature review

Detail, lower left angels bearing the Virgin; In 1703 the façade of Santa Maria dell’Umilta was rebuilt by Carlo Fontana; this included the Felici relief. The facade was greatly altered when rebuilt in 1859 by Andrea Busiri Vici, architect of Pius IX; only the relief and flanking pilasters remain of Fontana's design

Role of Progressive Widening of the Temporal Excitable Gap for Perpetuation of Atrial Fibrillation in the Goat

Background: Previous studies suggest that a short temporal excitable gap exists between the fibrillation waves during atrial fibrillation (AF). The aim of this study was to investigate the role of that gap in the development of sustained AF in goats. Methods and Results: Eight female goats were instrumented with left atrium (LA) electrodes, and sustained AF (>24 h) was induced by intermittent rapid atrial pacing for 9.3 +/- 4.6 days. In the process of sustained AF development, the atrial effective refractory period (AERP), refractory period during AF (RPAF), mean AF cycle length (AFCL), temporal excitable gap during AF (EG(AF)=AFCL-RPAF) and degree of fractionation of fibrillation electrograms at LA were studied. When the induced AF lasted for 3-10min, AFCL, RPAF and EG(AF) were 98.3 +/- 11.0 ms, 90.5 +/- 13.2 ms and 7.8 +/- 2.4 ms, respectively. During sustained AF, the values were 84.9 +/- 5.2 ms, 63.0 +/- 4.8 ms and 21.9 +/- 3.5ms, respectively (

HIB/SPOP inhibits Ci/Gli-mediated tumorigenesis by modulating the RNA Polymerase II components stabilities

Summary: Hedgehog (Hh) signaling mediated by transcription factor Ci/Gli plays a vital role in embryonic development and adult tissue homeostasis in invertebrates and vertebrates, whose dysregulation leads to many human disorders, including cancer. However, till now, cofactors of Ci/Gli which can affect tumorigenesis are not well known. Here, through genetic screen, we find overexpression of active Ci alone is not sufficient to generate tumor-like eye phenotype in Drosophila, however, its overexpression combined with knockdown of hib causes a striking tumor-like big eye phenotype. Mechanistically, HIB/SPOP inhibits Ci/Gli-mediated tumorigenesis by modulating the RNA polymerase II (RNAPII) components Rpb3/Rpb7 stabilities in E3 ligase dependent manner. In addition, Ci/Gli can promote HIB/SPOP-mediated Rpb7/Rpb3 degradation. Taken together, our results indicate Ci/Gli needs to hook up with suitable RNAPII together to achieve the tumor-like eye phenotype and HIB/SPOP plays dual roles through controlling Ci/Gli and Rpb3/Rpb7 protein stabilities to temper Ci/Gli/RNAPII-mediated tumorigenesis

Additional file 1: of Increased CD160 expression on circulating natural killer cells in atherogenesis

Figure S1. The representative dot plots showing the purity of freshly isolated NK cells from a donor. The percentage of CD3-CD56 + NK cells before and after sorting was shown in each graph

Additional file 3: of Increased CD160 expression on circulating natural killer cells in atherogenesis

Figure S3. A-C. The correlation analyses between the percentage of circulating CD160+ NK cells and serum levels of IFN-γ, TNF-ι and IL-6 in HC population. D. The correlation analyses between the percentage of circulating CD160+ NK cells and peripheral NK cell percentage in HC population. Spearman correlation test (A-D)