Direct imaging of short-range order and its impact on deformation in Ti-6Al.

Chemical short-range order (SRO) within a nominally single-phase solid solution is known to affect the mechanical properties of alloys. While SRO has been indirectly related to deformation, direct observation of the SRO domain structure, and its effects on deformation mechanisms at the nanoscale, has remained elusive. Here, we report the direct observation of SRO in relation to deformation using energy-filtered imaging in a transmission electron microscope (TEM). The diffraction contrast is enhanced by reducing the inelastically scattered electrons, revealing subnanometer SRO-enhanced domains. The destruction of these domains by dislocation planar slip is observed after ex situ and in situ TEM mechanical testing. These results confirm the impact of SRO in Ti-Al alloys on the scale of angstroms. The direct confirmation of SRO in relationship to dislocation plasticity in metals can provide insight into how the mechanical behavior of concentrated solid solutions by the material's thermal history

Deformation and failure of the CrCoNi medium-entropy alloy subjected to extreme shock loading

The extraordinary work hardening ability and fracture toughness of the face-centered cubic (fcc) high-entropy alloys render them ideal candidates for many structural applications. Here, the deformation and failure mechanisms of an equiatomic CrCoNi medium-entropyalloy (MEA) were investigated by powerful laser-driven shock experiments. Multiscale characterization demonstrates that profuse planar defects including stacking faults, nanotwins, and hexagonal nanolamella were generated during shock compression, forming a three-dimensional network. During shock release, the MEA fractured by strong tensile deformation and numerous voids was observed in the vicinity of the fracture plane. High defect populations, nanorecrystallization, and amorphization were found adjacent to these areas of localized deformation. Molecular dynamics simulations corroborate the experimental results and suggest that deformation-induced defects formed before void nucleation govern the geometry of void growth and delay their coalescence. Our results indicate that the CrCoNi-based alloys are impact resistant, damage tolerant, and potentially suitable in applications under extreme conditions.Fil: Zhao, Shiteng. Beihang University; China. Tianmushan Laboratory; ChinaFil: Sheng, Yin. Lawrence Berkeley National Laboratory; Estados UnidosFil: Xiao, Liang. Beihang University; ChinaFil: Fuhua, Cao. Chinese Academy of Sciences; República de ChinaFil: Qin, Yu. Lawrence Berkeley National Laboratory; Estados UnidosFil: Ruopeng, Zhang. Lawrence Berkeley National Laboratory; Estados UnidosFil: Lanhong, Dai. Chinese Academy of Sciences; República de ChinaFil: Ruestes, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina. Instituto Madrileño de Estudios Avanzados; EspañaFil: Ritchie, Robert. Lawrence Berkeley National Laboratory; Estados Unidos. University of California at Berkeley; Estados UnidosFil: Minor, Andrew M.. Lawrence Berkeley National Laboratory; Estados Unidos. University of California at Berkeley; Estados Unido

py4DSTEM: a software package for multimodal analysis of four-dimensional scanning transmission electron microscopy datasets

Scanning transmission electron microscopy (STEM) allows for imaging, diffraction, and spectroscopy of materials on length scales ranging from microns to atoms. By using a high-speed, direct electron detector, it is now possible to record a full 2D image of the diffracted electron beam at each probe position, typically a 2D grid of probe positions. These 4D-STEM datasets are rich in information, including signatures of the local structure, orientation, deformation, electromagnetic fields and other sample-dependent properties. However, extracting this information requires complex analysis pipelines, from data wrangling to calibration to analysis to visualization, all while maintaining robustness against imaging distortions and artifacts. In this paper, we present py4DSTEM, an analysis toolkit for measuring material properties from 4D-STEM datasets, written in the Python language and released with an open source license. We describe the algorithmic steps for dataset calibration and various 4D-STEM property measurements in detail, and present results from several experimental datasets. We have also implemented a simple and universal file format appropriate for electron microscopy data in py4DSTEM, which uses the open source HDF5 standard. We hope this tool will benefit the research community, helps to move the developing standards for data and computational methods in electron microscopy, and invite the community to contribute to this ongoing, fully open-source project

Insights into the reduction of antibiotic-resistant bacteria and mobile antibiotic resistance genes by black soldier fly larvae in chicken manure

The increasing prevalence of antibiotic-resistant bacteria (ARB) from animal manure has raised concerns about the potential threats to public health. The bioconversion of animal manure with insect larvae, such as the black soldier fly larvae (BSFL, Hermetia illucens [L.]), is a promising technology for quickly attenuating ARB while also recycling waste. In this study, we investigated BSFL conversion systems for chicken manure. Using metagenomic analysis, we tracked ARB and evaluated the resistome dissemination risk by investigating the co-occurrence of antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and bacterial taxa in a genetic context. Our results indicated that BSFL treatment effectively mitigated the relative abundance of ARB, ARGs, and MGEs by 34.9%, 53.3%, and 37.9%, respectively, within 28 days. Notably, the transferable ARGs decreased by 30.9%, indicating that BSFL treatment could mitigate the likelihood of ARG horizontal transfer and thus reduce the risk of ARB occurrence. In addition, the significantly positive correlation links between antimicrobial concentration and relative abundance of ARB reduced by 44.4%. Moreover, using variance partition analysis (VPA), we identified other bacteria as the most important factor influencing ARB, explaining 20.6% of the ARB patterns. Further analysis suggested that antagonism of other bacteria on ARB increased by 1.4 times, while nutrient competition on both total nitrogen and crude fat increased by 2.8 times. Overall, these findings provide insight into the mechanistic understanding of ARB reduction during BSFL treatment of chicken manure and provide a strategy for rapidly mitigating ARB in animal manure.This work was funding by the National Natural Science Foundation of China (41977279), the Fundamental Research Funds for the Central Universities (2662020SKPY002 and 2662022SKYJ006), the Key Technology R & D Program of Hubei Province (2021BBA258) and the Major Project of Hubei Hongshan Laboratory (2022hszd013).Peer ReviewedPostprint (published version

Shock-Induced Amorphization in Covalently Bonded Solids

Pulsed lasers with a power of the order of terawatts, once deposited on a target surface, will launch a stress pulse that propagates into material. Owing to the ultrashort duration of the laser pulses, unprecedented experimental conditions which combine high pressures (and/or shear stresses), strain rates and temperatures can be generated in materials, yielding a yet unexplored regime of study: materials science at extremes. High-power, short-duration, laser-driven, shock compression and recovery experiments were carried out on four covalently bonded materials, namely, silicon (Si), germanium (Ge), boron carbide (B4C) and silicon carbide (SiC). These materials were chosen because of their high Peierls-Nabarro stress and negative Clapeyron slope. The profile of the shock waves was measured by a velocity interferometer system for any reflectors (VISAR). The shock deformation microstructure has been revealed by high resolution transmission electron microscopy and all the materials exhibit shock-induced amorphization. For Si and Ge with [001] orientation, two distinct amorphous regions were identified: (i) a bulk amorphous layer close to the surface and (ii) amorphous bands initially aligned with {111} slip planes. The VISAR measurements show that the estimated thresholds for such a crystalline-to-amorphous transition is estimated to be ~10 GPa (for silicon) and ~4 GPa (for germanium). Further increase of the shock stress leads to the crystallization of amorphous domain into nanocrystals with high density of nano-twins. For polycrystalline boron carbide, only amorphous bands inclined to the direction of shock wave propagation have been observed at a shock stress above ~45 GPa. At lower shock stress, planar faults have been seen below the shocked surface. For [0001] oriented monocrystalline silicon carbide, in addition to the amorphous bands inclined to the shock direction, some amorphous bands perpendicular to the direction of shock wave propagation were observed. We propose that the amorphization is produced by the combined effect of high magnitude hydrostatic and shear stresses under dynamic shock compression. This study reveals that amorphization is a general inelastic deformation mechanisms in covalently bonded elements and compounds subjected to shock compression. Their formation yields a decrease in the overall hydrostatic and deviatoric elastic energy. Shock-induced defects play a very important role in the onset of amorphization. Calculations of the free energy changes with pressure and shear, using the Patel-Cohen methodology, agree with the experimental results. Molecular dynamics simulation corroborates the amorphization, showing that it is initiated by the nucleation and propagation of partial dislocations. The nucleation of amorphization is analyzed by classical nucleation theory