Spatial Single-Cell Sequencing of Meiosis I Arrested Oocytes Indicates Acquisition of Maternal Transcripts From the Soma

Maternal RNAs are stored from minutes to decades in oocytes throughout meiosis I arrest in a transcriptionally quiescent state. Recent reports, however, propose a role for nascent transcription in arrested oocytes. Whether arrested oocytes launch nascent transcription in response to environmental or hormonal signals while maintaining the meiosis I arrest remains undetermined. We test this by integrating single-cell RNA sequencing, RNA velocity, and RNA fluorescence in situ hybridization on C. elegans meiosis I arrested oocytes. We identify transcripts that increase as the arrested meiosis I oocyte ages, but rule out extracellular signaling through ERK MAPK and nascent transcription as a mechanism for this increase. We report transcript acquisition from neighboring somatic cells as a mechanism of transcript increase during meiosis I arrest. These analyses provide a deeper view at single-cell resolution of the RNA landscape of a meiosis I arrested oocyte and as it prepares for oocyte maturation and fertilization

Effect of post-peak cyclic load on mechanics and seepage characteristics of sandstone under different confining pressures

The mining disturbance causes the rock mass in a certain range of the coal seam floor to be in a post-peak state. Due to the excavation of adjacent roadways and the mining of coal seams, the post-peak rock mass undergoes a cyclic loading-unloading process. In order to explore the influence of post-peak cyclic loading on rock mass structure under different in-situ stress environments and clarify the gestation process of disasters such as water inrush from coal seam floor, post-peak cyclic loading tests of sandstone under 5,10,15,20,25 MPa confining pressure were carried out based on Rock Top multi-field coupling tester. The results show that: ① Before the post-peak cyclic loading, the unit permeability of rock shows a rapid decline-tend to be stable-sudden rise-sudden decline-tend to be stable. In the post-peak cycle stage, the unit permeability of rock is almost inverted with the axial load. ② Rock elastic modulus, crack closure stress, crack initiation stress, damage stress, peak stress and residual stress are positively correlated with confining pressure, while Poisson 's ratio increases first and then decreases with the increase of confining pressure. ③ Under the confining pressure of 5 and 10 MPa, the intermittent failure of rock occurs, and the brittle failure characteristics are weakened. Under the confining pressure of 15, 20 and 25 MPa, the brittle failure characteristics of rock are obvious, and the failure characteristics of rock are determined by the properties of rock itself. ④ The post-peak axial load mainly promotes the increase of rock permeability, but the promotion effect is weaker than that of confining pressure on rock permeability, and confining pressure is the dominant factor affecting the post-peak permeability change of rock. ⑤ Under different confining pressures, a through-shear crack occurs in all rocks, but with the increase of confining pressure, the degree of rock failure gradually weakens and the failure mode tends to be simple

Optimasi Portofolio Resiko Menggunakan Model Markowitz MVO Dikaitkan dengan Keterbatasan Manusia dalam Memprediksi Masa Depan dalam Perspektif Al-Qur`an

Risk portfolio on modern finance has become increasingly technical, requiring the use of sophisticated mathematical tools in both research and practice. Since companies cannot insure themselves completely against risk, as human incompetence in predicting the future precisely that written in Al-Quran surah Luqman verse 34, they have to manage it to yield an optimal portfolio. The objective here is to minimize the variance among all portfolios, or alternatively, to maximize expected return among all portfolios that has at least a certain expected return. Furthermore, this study focuses on optimizing risk portfolio so called Markowitz MVO (Mean-Variance Optimization). Some theoretical frameworks for analysis are arithmetic mean, geometric mean, variance, covariance, linear programming, and quadratic programming. Moreover, finding a minimum variance portfolio produces a convex quadratic programming, that is minimizing the objective function ðð¥with constraintsð ð 𥠥 ðandð´ð¥ = ð. The outcome of this research is the solution of optimal risk portofolio in some investments that could be finished smoothly using MATLAB R2007b software together with its graphic analysis

Measurement of t(t)over-bar normalised multi-differential cross sections in pp collisions at root s=13 TeV, and simultaneous determination of the strong coupling strength, top quark pole mass, and parton distribution functions

Peer reviewe

Search for supersymmetry in events with one lepton and multiple jets in proton-proton collisions at root s=13 TeV

Peer reviewe

An embedding technique to determine ττ backgrounds in proton-proton collision data

An embedding technique is presented to estimate standard model tau tau backgrounds from data with minimal simulation input. In the data, the muons are removed from reconstructed mu mu events and replaced with simulated tau leptons with the same kinematic properties. In this way, a set of hybrid events is obtained that does not rely on simulation except for the decay of the tau leptons. The challenges in describing the underlying event or the production of associated jets in the simulation are avoided. The technique described in this paper was developed for CMS. Its validation and the inherent uncertainties are also discussed. The demonstration of the performance of the technique is based on a sample of proton-proton collisions collected by CMS in 2017 at root s = 13 TeV corresponding to an integrated luminosity of 41.5 fb(-1).Peer reviewe

Measurement of the Splitting Function in &ITpp &ITand Pb-Pb Collisions at root&ITsNN&IT=5.02 TeV

Data from heavy ion collisions suggest that the evolution of a parton shower is modified by interactions with the color charges in the dense partonic medium created in these collisions, but it is not known where in the shower evolution the modifications occur. The momentum ratio of the two leading partons, resolved as subjets, provides information about the parton shower evolution. This substructure observable, known as the splitting function, reflects the process of a parton splitting into two other partons and has been measured for jets with transverse momentum between 140 and 500 GeV, in pp and PbPb collisions at a center-of-mass energy of 5.02 TeV per nucleon pair. In central PbPb collisions, the splitting function indicates a more unbalanced momentum ratio, compared to peripheral PbPb and pp collisions.. The measurements are compared to various predictions from event generators and analytical calculations.Peer reviewe