An oxygen pool from YBaCo4O7-based oxides for soot combustion

Acknowledgements This work is financially supported by the National Natural Science Foundation of China (no. 21477046, 21277060 and 21547007). Open Access via RSC Gold4GoldPeer reviewe

Сильные и слабые теоремы сходимости для общих задач смешанного равновесия и общих задач вариационного неравенства и задач с фиксированной точкой для двух нерасширяющихся полугрупп в гильбертовых пространствах

Вводятся некоторые итерационные алгоритмы нахождения общего элемента множества решений общей задачи смешанного равновесия и множества решений общего вариационного неравенства для двух коэрцитивных отображений и множества общих неподвижных точек двух нерасширяющихся полугрупп в гильбертовом пространстве. Получены как сильные, так и слабые теоремы сходимости для последовательностей, порожденных этими итерационными процессами в гильбертовых пространствах. Результаты настоящего исследования улучшают и расширяют данные многих других авторов

MicroRNAs control mRNA fate by compartmentalization based on 3 ' UTR length in male germ cells

Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying post-transcriptional regulation in vivo because it involves a large number of transcripts that are physically sequestered in ribonucleoprotein particles (RNPs) and thus subjected to delayed translation. To explore how small RNAs regulate mRNA fate, we conducted RNA-Seq analyses to determine not only the levels of both mRNAs and small noncoding RNAs, but also their cytoplasmic compartmentalization during spermiogenesis. Result: Among all small noncoding RNAs studied, miRNAs displayed the most dynamic changes in both abundance and subcytoplasmic localization. mRNAs with shorter 3' UTRs became increasingly enriched in RNPs from pachytene spermatocytes to round spermatids, and the enrichment of shorter 3' UTR mRNAs in RNPs coincided with newly synthesized miRNAs that target these mRNAs at sites closer to the stop codon. In contrast, the translocation of longer 3' UTR mRNAs from RNPs to polysomes correlated with the production of new miRNAs that target these mRNAs at sites distal to the stop codon. Conclusions: miRNAs appear to control cytoplasmic compartmentalization of mRNAs based on 3' UTR length. Our data suggest that transcripts with longer 3' UTRs tend to contain distal miRNA binding sites and are thus targeted to polysomes for translation followed by degradation. In contrast, those with shorter 3' UTRs only possess proximal miRNA binding sites, which, therefore, are targeted into RNPs for enrichment and delayed translation

Adrenaline inhibited cell proliferation and regulated expression of TGF-beta1 and bFGF in cultured human hypertrophic scar fibroblasts via alpha-receptor

Adrenaline has been shown to modulate proliferation of mouse fibroblasts, adventitial fibroblasts and synovial B (fibroblasts-like) cells. However, little is known about the response of cultured human hypertrophic scar fibroblasts to adrenaline. In this study, we investigated cell proliferation and involved mechanisms in hypertrophic scar fibroblasts in response to adrenaline. Population doubling time (PDT) assay and MTT assay were performed to determine the cell proliferation and cell viability, respectively. The expression of bFGF and TGF- ß1 was measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results showed that adrenaline inhibited proliferation of normal and hypertrophic scar fibroblasts in a dose-dependent manner. Moreover, adrenaline up-regulated the expression of bFGF and down-regulated the expression of TGF- ß1 in normal and hypertrophic scar fibroblasts. Interestingly incubation with the a receptor antagonist regitine indicated that adrenaline mediated inhibition of cell proliferation and regulation of TGF-ß1 and bFGF in cultured normal and hypertrophic scar fibroblasts were mediated by the a receptor. These studies suggest that adrenaline inhibits proliferation and alters the expression of TGF-ß1 and bFGF in human hypertrophic scar fibroblast involving an a receptor mediated pathway

Few-shot image classification : current status and research trends

Conventional image classification methods usually require a large number of training samples for the training model. However, in practical scenarios, the amount of available sample data is often insufficient, which easily leads to overfitting in network construction. Few-shot learning provides an effective solution to this problem and has been a hot research topic. This paper provides an intensive survey on the state-of-the-art techniques in image classification based on few-shot learning. According to the different deep learning mechanisms, the existing algorithms are di-vided into four categories: transfer learning based, meta-learning based, data augmentation based, and multimodal based methods. Transfer learning based methods transfer useful prior knowledge from the source domain to the target domain. Meta-learning based methods employ past prior knowledge to guide the learning of new tasks. Data augmentation based methods expand the amount of sample data with auxiliary information. Multimodal based methods use the information of the auxiliary modal to facilitate the implementation of image classification tasks. This paper also summarizes the few-shot image datasets available in the literature, and experimental results tested by some representative algorithms are provided to compare their performance and analyze their pros and cons. In addition, the application of existing research outcomes on few-shot image classification in different practical fields are discussed. Finally, a few future research directions are iden-tified. © 2022 by the authors. Licensee MDPI, Basel, Switzerland

Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry

<p>Abstract</p> <p>Background</p> <p><it>Da Chengqi Tang </it>(DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from <it>Fructus Aurantii Immaturus</it>. The present study developed an LC-MS/MS method to characterize two pairs of flavanone 7-glycoside isomers, i.e., hesperidin versus neohesperidin and naringin versus isonaringin.</p> <p>Methods</p> <p>After solid phase purification, components in sample were separated on a Agilent zorbax SB-C18 (5 μm, 250 mm × 4.6 mm) analytical column. ESI-MS and quasi-MSⁿwere performed in negative ion mode to obtain structural data of these two pairs of flavanone 7-glycoside isomers. Moreover, UV absorption was measured.</p> <p>Results</p> <p>There was no intra-pairs difference in the UV-Vis and MS/MS spectra of the two pairs of 7-glycoside isomers, whereas the mass spectrometry fragmentation pathways between pairs were different.</p> <p>Conclusion</p> <p>The present study developed a LC-MS/MS method to explore the inter- and intra-pair difference of two pairs of flavanone 7-glycoside isomers.</p

Involvement of microRNA-93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer cells

AbstractThe mechanisms underlying ovarian cancer cell resistance to cisplatin (CDDP) are not fully understood. MicroRNAs (miRNAs) play important roles in tumorigenesis and drug resistance. In this paper, we utilized microRNA array and real-time PCR to show that miR-93 is significantly up-regulated in cisplatin-resistant ovarian cancer cells. In vitro assays show that over-expression and knock-down of miR-93 regulate apoptotic activity, and thereby cisplatin chemosensitivity, in ovarian cells. Furthermore, we found that miR-93 can directly target PTEN, and participates in the regulation of the AKT signaling pathway. MiR-93 inversely correlates with PTEN expression in CDDP-resistant and sensitive human ovarian cancer tissues. These results may have implications for therapeutic strategies aiming to overcome ovarian cancer cell resistance to cisplatin