Some new constructions of orthogonal designs

In this paper we construct OD(4pqr(q+1); pqr, pqr, pqr, pqr, pqr+1, pqr+1, pqr+1, pqr+1) for each core order q ≡ 3(mod 4), r ≥ 0 or q = 1, p odd, p ≤ 21 and p ∈ {25, 49}, and COD(2qr(q + 1); qr, qr, qr+1, qr+1) for any prime power q ≡ 1 (mod 4) (including q = 1), r ≥ 0

Identification of a mutated BHK-21 cell line that became less susceptible to Japanese encephalitis virus infection

The pathogenesis of Japanese encephalitis virus (JEV) is not definitely elucidated as the initial interaction between virus and host cell receptors required for JEV infection is not clearly defined yet. Here, in order to discover those membrane proteins that may be involved in JEV attachment to or entry into virus permissive BHK-21 cells, a chemically mutated cell line (designated 3A10-3F) that became less susceptible to JEV infection was preliminarily established and selected by repeated low moi JEV challenges and RT-PCR detection for viral RNA E gene fragment. The susceptibility to JEV of 3A10-3F cells was significantly weakened compared with parental BHK-21 cells, verified by indirect immunofluorescence assay, virus plague formation assay, and flow cytometry. Finally, two-dimensional electrophoresis (2-DE) coupled with LC-MS/MS was utilized to recognize the most differentially expressed proteins from membrane protein extracts of 3A10-3F and BHK-21 cells respectively. The noted discrepancy of membrane proteins included calcium binding proteins (annexin A1, annexin A2), and voltage-dependent anion channels proteins (VDAC 1, VDAC 2), suggesting that these molecules may affect JEV attachment to and/or entry into BHK-21 cells and worthy of further investigation

Does Japanese encephalitis virus share the same cellular receptor with other mosquito-borne flaviviruses on the C6/36 mosquito cells?

Japanese encephalitis virus (JEV) is a member of mosquito-borne Flaviviridae. To date, the mechanisms of the early events of JEV infection remain poorly understood, and the cellular receptors are unidentified. There are evidences that the structure of the virus attachment proteins (VAP), envelope glycoprotein of mosquito-borne flaviviruses is very similar, and the vector-virus interaction of mosquito-borne flaviviruses is also very similar. Based on the studies previously demonstrated that the similar molecules present on the mosquito cells involved in the uptake process of JEV, West Nile virus (WNV) and Dengue virus (DV), it is proposed that the same receptor molecules for mosquito-borne flaviviruses (JEV, WNV and DV) may present on the surface of C6/36 mosquito cells. By co-immunoprecipitation assay, we investigated a 74-KDa protein on the C6/36 cells binds JEV, and the mass spectrometry results indicated it may be heat shock cognate protein 70(HSC70) from Aedes aegypti. Based upon some other viruses use of heat shock protein 70 (HSP70) family proteins as cell receptors, its possible HSC70's involvement in the fusion of the JEV E protein with the C6/36 cells membrane, and known form of cation channels in the interaction of HSC70 with the lipid bilayer, it will further be proposed that HSC70 as a penetration receptor mediates JEV entry into C6/36 cells

Some new constructions of orthogonal designs

In this paper we construct OD(4pq r (q + 1); pq r, pq r, pq r, pq r, pq r+1, pq r+1, pq r+1, pq r+1) for each core order q ≡ 3(mod 4), r ≥ 0 or q = 1, p odd, p ≤ 21 and p ∈ {25, 49}, and COD(2q r (q + 1); q r, q r, q r+1, q r+1) for any prime power q ≡ 1(mod 4) (including q = 1), r ≥ 0.

Perceived occupational stressors among nurses in a level-1 trauma center under normalized COVID-19 epidemic prevention and control in China: A qualitative study

Background: Nurses in trauma centers encounter emergency patients and are exposed to occupational stressors under normalized pandemic control and prevention. Objective: The objective of this study was to examine the occupational stressors that nurses perceived during the COVID-19 pandemic in a level-1 trauma center in China. Methods: From December 2020 to May 2021, a qualitative study was conducted, employing conventional content analysis as the research methodology. A total of twelve nurses were purposefully selected from a trauma center at a major hospital in Beijing to participate in semi-structured interviews (40–50 min’ duration). The data collected in this study were analyzed using the conventional content analysis approach proposed by Graneheim and Lundman. Results: After analyzing the data, two themes emerged: 1) Critical conditions under normalized pandemic prevention and control; 2) Concerns about loved ones and colleagues, along with financial problems. Both these themes further revealed four categories: the complexity and uncertainty of epidemic prevention and health hazards in the former, and concerns for and from families and colleagues, and financial stress in the latter. Conclusions: The results of this study provide valuable insights into the practical implementation of epidemic prevention and control measures in Chinese trauma centers and shed light on the psychological stress faced by nurses. Improving the tripartite support system of hospital, family, and society, and providing emotional support may help reduce stress among nurses, thereby improving their health and care quality

Performance study of piezoelectric energy harvester for vertical shaft hoisting system

For the characteristics of irregular low-frequency and broad-frequency vibration in the operation of the cage of vertical shaft hoisting system, a variable-section bending beam piezoelectric vibration harvester is proposed to broaden the operating band of the harvester and improve the energy collection efficiency of the harvester for irregular low-frequency and broad-frequency vibration in the vertical shaft hoisting system. The structural finite element analysis of the harvester was carried out, and the experimental test platform was built. Based on simulation and experiment, the difference of the power generation performance between the harvester and the trapezoidal beam and the traditional rectangular beam was explored, and the energy supply test was carried out with the energy harvester. The results show that the strain of the beam is more uniform and the overall output response of piezoelectric material is better. At the same time, the resonance frequency is low, the open circuit output voltage is high, and there are many obvious peak values, which can effectively realize the broadening of acquisition frequency band. When the end bending angle of the piezoelectric vibrator is 40°, and the additional mass is 2.14 g, the maximum load output voltage of the harvester is 34.4 V and the maximum output power can reach 14.7 mW under the standard energy interface circuit and the optimal load resistance is 80 kΩ. The power supply test of the wireless node verifies its energy conversion efficiency and feasibility of energy supply, which can meet the power supply requirements of the wireless low-power sensor for mining