L-Karnitin ve Magnezyumun Deneysel Diyabet Oluşturulan Ratların Dokularında Oksidatif Stres ile Sitokinler Üzerine Etkilerinin Araştırılması

ÖZET:Buçalışmada, deneysel diyabet oluşturulmuş ratlarda L-karnitin ve magnezyumun(Mg) doku malondialdehit (MDA), 8-OHdG ve sitokin (TNFα, IL-6) düzeyleriüzerine etkilerinin belirlenmesi amaçlanmaktadır. Seksen adet Wistar albinoerkek rattan elde edilen karaciğer ve böbrek dokuları kullanılmıştır.Hayvanlar, her biri 10’ar adetten oluşan 8 çalışma grubuna ayrılmıştır: 1. grupkontrol olarak; 2. grup 50 mg/kg (canlı ağırlık) i.p. olarak STZ verilen; 3.grup 125 mg/kg (canlı ağırlık)/gün MgSO4 şeklinde Mg verilen; 4.grup 300 mg/kg (canlı ağırlık)/gün L-karnitin verilen; 5. grup; 125 mg/kg(canlı ağırlık)/gün Mg + 300 mg/kg (canlı ağırlık)/gün L-karnitin verilen; 6.grup 50 mg/kg i.p. olarak STZ + 125 mg/kg (canlı ağırlık)/gün Mg verilen; 7.grup 50 mg/kg i.p. olarak STZ + 300 mg/kg (canlı ağırlık)/gün Lkarnitinverilen; 8. grup 50 mg/kg i.p. olarak STZ + 125 mg/kg (canlı ağırlık)/gün Mg +300 mg/kg (canlı ağırlık)/gün L-karnitin dört hafta verilmiştir. Karaciğer veböbrek MDA, 8- OHdG, TNF-α ve IL-6 düzeyleri yönünden kontrol grubu ile Mg,karnitin ve Mg+karnitin grupları arasında istatistiki önemde bir farkbelirlenmemiştir. En yüksek MDA, 8-OHdG, TNF-α ve IL-6 düzeyleri sadece diyabetoluşturulan grupta saptanmıştır. Diyabetli hayvanlara hem Mg’un hem dekarnitin’in ayrı ayrı veya kombine verilmesi ile diyabete bağlı artan lipidperoksidasyonu, DNA harabiyeti ve sitokin düzeyleri istatistiki önemde azalmagöstermiştir. Sonuç olarak Mg ve karnitinin, özellikle kombine şekilde,antidiyabetik etkilere sahip olabileceği kanaatine varılmıştır.ABSTRACT: In thisstudy evaluated the effects of L-carnitine and magnesium (Mg) on tissuemalondialdehide (MDA), 8-OHdG and cytokines (TNFα, IL-6) levels in experimentaldiabetes in rats. Eighty Wistar albino rats were divided into eight groups often. The groups received the following treatment: group 1: control group; 2 mldistilled water (by gavage), group 2: 50 mg/kg (b.w.) i.p. STZ, group 3: 125mg/kg (b.w.) Mg, group 4: 300 mg/kg (b.w.) L-carnitine, group 5: 125 mg/kg(b.w.) Mg +300 mg/kg (b.w.) L-carnitine, group 6: 50 mg/kg (b.w.) STZ +125mg/kg (b.w.) Mg, group 7: 50 mg/kg (b.w.) STZ +300 mg/kg (b.w.) L-carnitine andgroup 8: 50 mg/kg (b.w.) STZ +125 mg/kg (b.w.) Mg+300 mg/kg (b.w.) L-carnitinewas administered for four weeks. There was no statistically significant differencebetween control group and Mg, carnitine and Mg + carnitine groups in terms ofliver and kidney MDA, 8- OHdG, TNF-α and IL-6 levels. The highest levels ofMDA, 8-OHdG, TNF-α and IL-6 were detected only in the diabetic group. Increasedlipid peroxidation, DNA damage and cytokine levels due to diabetes, bothindividually or in combination with both Mg and carnitine, were significantlyreduced in diabetic animals. As a result, it has been concluded that Mg andLcarnitine may have antidiabetic effects, especially in combination.</p

The Effect of Boron on Some Biochemical Parameters in Experimental Diabetic Rats

In this study, we evaluated the effect of boron (B) as boric acid (BA) on body weight (b.w.); blood glucose; plasma insulin; lipase and paraoxonase (PON1) activities; and serum triglyceride, total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, lipid peroxidation (MDA), and total antioxidant capacity (TAC) in streptozotocin (STZ)-induced experimental diabetes in rats. Sixty Wistar albino rats (200-250 g) were divided into six groups of ten. The groups received the following treatment: group 1, control group; group 2, 50 mg/kg (b.w.) i.p. STZ-induced diabetes; group 3, 5 mg/kg (b.w.) B; group 4, 10 mg/kg (b.w.) B; group 5, diabetes + 5 mg/kg (b.w.) B; and group 6, diabetes + 10 mg/kg (b.w.) B. The experiment lasted 4 weeks. Increased serum MDA levels with diabetes were significantly reduced and although it is not statistically significant, serum TAC levels approached to values of control group; also, insignificant increases were observed in HDL cholesterol levels in experimental diabetic rats with treatment 5 and 10 mg/kg B. Furthermore, body weight, plasma insulin, and lipase activities increased insignificantly, blood glucose and serum LDL cholesterol decreased significantly, and total cholesterol levels decreased insignificantly in the diabetes + 10 mg/kg B group. There was no difference between the groups in terms of plasma PON1 activities and serum triglyceride levels. In conclusion, B may have beneficial effects on some biochemical parameters changes in experimental diabetes, and in order to determine the full effect of this element on the metabolism, further studies are required which use various dosages and compounds of B

The effects of magnesium and L-carnitine on some biochemical parameters in experimental diabetic rats

In this study, the effects of magnesium (Mg) and L-carnitine on body weight, blood glucose, plasma lipase and paraoxonase activities, serum lipids, lipid peroxidation (MDA) and total antioxidant capacity (TAC) were determined in rats with experimental diabetes mellitus induced by streptozotocin. Eighty Wistar albino male rats (200-250 g) were divided into eight groups of ten. First group (control) received 2 ml distilled water; group 2 received 50 mg/kg (b.w., i.p.) STZ; Group 3 received 125 mg/kg (b.w.) Mg; group 4 received 300 mg/kg (b.w.) L-carnitine; group 5 received 125 mg/kg (b.w.) Mg+300 mg/kg (b.w.) L-carnitine; group 6 received 50 mg/kg (b.w.) STZ+125 mg/kg (b.w.) Mg; Group 7 received 50 mg/kg (b.w.) STZ+300 mg/kg (b.w.) L-carnitine; Group 8 received 50 mg/kg (b.w.) STZ+125 mg/kg (b.w.) Mg+300 mg/kg (b.w.) L-carnitine for four weeks. In rats with diabetes, oral administration of 125 mg/kg Mg and 300 mg/kg L-carnitine separately, was found to have no effect on body weight, blood glucose, serum total cholesterol, HDL and LDL-cholesterol and TAC. In diabetic rats, serum MDA levels decreased with the administration of both substances separately and/or in combination, and triglyceride levels decreased with only L-carnitine and Mg+L-carnitine administration. Especially with the combined application of Mg and L-carnitine, the high blood glucose levels determined in rats with diabetes decreased significantly. As a result, it was concluded that Mg and L-carnitine may have antidiabetic effects, especially in combination