Estudio de obra y propuesta de conservación de una pintura sobre tabla del S. XIX

[EN] The objective of this final work is the study and proposal of intervention, of a painting on wood. The theme of the work is a still life Dutch-inspired. It dates back to the work of the 19th century. It comes from a gallery located in the barrio del Carmen in Valencia. It presents a regular condition. It has hemming in the back, this being a system of structural reinforcement very common from the end of the 18th century, considered over a long period of time as a seal of quality of the Works. It presents a darkened varnish, applied with the intention of evoking an aged appearance of the age of this work as this also very common practice in the 19th century. Finally, the work has a Baroque gold frame. It have damage in the reliefs, as well as an inappropriate restraint system and work.El objetivo de este trabajo de fin de grado es el estudio y propuesta de intervención de una pintura sobre tabla. La temática de la obra es un bodegón inspirado en el estilo holandés, realizada en el s.XIX, y que proviene de una galería situada en el barrio del Carmen de Valencia. Presenta un estado de conservación aceptable. Posee un engatillado en la parte posterior, siendo este un sistema de refuerzo estructural muy común a partir del s. XVIII, considerado durante un largo periodo de tiempo como sello de calidad de las obras. Presenta un barniz oscurecido, aplicado con la intención de evocar un aspecto envejecido, impropio de la edad de esta obra, siendo esta práctica también muy habitual en el s. XIX. Para finalizar, la obra posee un marco de estilo barroco, dorado, con una pátina coloreada. Presenta desperfectos en los relieves, además de un inadecuado sistema sujeción de la obra.TFG

Climate pattern-scaling set for an ensemble of 22 GCMs – adding uncertainty to the IMOGEN version 2.0 impact system

Global circulation models (GCMs) are the best tool to understand climate change, as they attempt to represent all the important Earth system processes, including anthropogenic perturbation through fossil fuel burning. However, GCMs are computationally very expensive, which limits the number of simulations that can be made. Pattern scaling is an emulation technique that takes advantage of the fact that local and seasonal changes in surface climate are often approximately linear in the rate of warming over land and across the globe. This allows interpolation away from a limited number of available GCM simulations, to assess alternative future emissions scenarios. In this paper, we present a climate pattern-scaling set consisting of spatial climate change patterns along with parameters for an energy-balance model that calculates the amount of global warming. The set, available for download, is derived from 22 GCMs of the WCRP CMIP3 database, setting the basis for similar eventual pattern development for the CMIP5 and forthcoming CMIP6 ensemble. Critically, it extends the use of the IMOGEN (Integrated Model Of Global Effects of climatic aNomalies) framework to enable scanning across full uncertainty in GCMs for impact studies. Across models, the presented climate patterns represent consistent global mean trends, with a maximum of 4 (out of 22) GCMs exhibiting the opposite sign to the global trend per variable (relative humidity). The described new climate regimes are generally warmer, wetter (but with less snowfall), cloudier and windier, and have decreased relative humidity. Overall, when averaging individual performance across all variables, and without considering co-variance, the patterns explain one-third of regional change in decadal averages (mean percentage variance explained, PVE, 34.25 ± 5.21), but the signal in some models exhibits much more linearity (e.g. MIROC3.2(hires): 41.53) than in others (GISS_ER: 22.67). The two most often considered variables, near-surface temperature and precipitation, have a PVE of 85.44 ± 4.37 and 14.98 ± 4.61, respectively. We also provide an example assessment of a terrestrial impact (changes in mean runoff) and compare projections by the IMOGEN system, which has one land surface model, against direct GCM outputs, which all have alternative representations of land functioning. The latter is noted as an additional source of uncertainty. Finally, current and potential future applications of the IMOGEN version 2.0 modelling system in the areas of ecosystem modelling and climate change impact assessment are presented and discussed

Plantio comprobatório de canjarana (Cabralea canjerana subsp. canjerana).

bitstream/item/101394/1/PA-1999-Carvalho-PlantioComprobatorio.pd

The Usage of ROOT for Online Monitoring in the ALICE DATE System

Presentation for CHEP2000Data Acquisition systems for HEP applications need constant monitoring (online and offline) of their data streams to accomplish several tasks: quality checking, tuning, statistics, pre-analysis. Monitoring tasks can and should use the same tools as data analysis products (conventions, libraries, environments) to reduce training, installation, development and support efforts and -at the same time - to strengthen the liaison between the online and the offline worlds. The ALICE DATE Data Acquisition system available today for R&D and for test beams is fully integrated with the ROOT environment. A simple DAQ-oriented approach and a more complex OO-based model have been developed to allow a variety of programming paradigms and to validate the complete life cycle of monitoring tools, both for online and offline environments

The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding

Background: The WW domain containing protein WWOX has been postulated to behave as a tumor suppressor in breast and other cancers. Expression of this protein is lost in over 70% of ER negative tumors. This prompted us to investigate the phenotypic and gene expression effects of loss of WWOX expression in breast cells. Methods: Gene expression microarrays and standard in vitro assays were performed on stably silenced WWOX (shRNA) normal breast cells. Bioinformatic analyses were used to identify gene networks and transcriptional regulators affected by WWOX silencing. Co-immunoprecipitations and GST-pulldowns were used to demonstrate a direct interaction between WWOX and SMAD3. Reporter assays, ChIP, confocal microscopy and in silico analyses were employed to determine the effect of WWOX silencing on TGFβ-signaling. Results: WWOX silencing affected cell proliferation, motility, attachment and deregulated expression of genes involved in cell cycle, motility and DNA damage. Interestingly, we detected an enrichment of targets activated by the SMAD3 transcription factor, including significant upregulation of ANGPTL4, FST, PTHLH and SERPINE1 transcripts. Importantly, we demonstrate that the WWOX protein physically interacts with SMAD3 via WW domain 1. Furthermore, WWOX expression dramatically decreases SMAD3 occupancy at the ANGPTL4 and SERPINE1 promoters and significantly quenches activation of a TGFβ responsive reporter. Additionally, WWOX expression leads to redistribution of SMAD3 from the nuclear to the cytoplasmic compartment. Since the TGFβ target ANGPTL4 plays a key role in lung metastasis development, we performed a meta-analysis of ANGPTL4 expression relative to WWOX in microarray datasets from breast carcinomas. We observed a significant inverse correlation between WWOX and ANGPTL4. Furthermore, the WWOX lo/ANGPTL4hi cluster of breast tumors is enriched in triple-negative and basal-like sub-types. Tumors with this gene expression signature could represent candidates for anti-TGFβ targeted therapies. Conclusions: We show for the first time that WWOX modulates SMAD3 signaling in breast cells via direct WW-domain mediated binding and potential cytoplasmic sequestration of SMAD3 protein. Since loss of WWOX expression increases with breast cancer progression and it behaves as an inhibitor of SMAD3 transcriptional activity these observations may help explain, at least in part, the paradoxical pro-tumorigenic effects of TGFβ signaling in advanced breast cancer.Facultad de Ciencias MédicasCentro de Investigaciones Inmunológicas Básicas y Aplicada

The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding

Background: The WW domain containing protein WWOX has been postulated to behave as a tumor suppressor in breast and other cancers. Expression of this protein is lost in over 70% of ER negative tumors. This prompted us to investigate the phenotypic and gene expression effects of loss of WWOX expression in breast cells. Methods: Gene expression microarrays and standard in vitro assays were performed on stably silenced WWOX (shRNA) normal breast cells. Bioinformatic analyses were used to identify gene networks and transcriptional regulators affected by WWOX silencing. Co-immunoprecipitations and GST-pulldowns were used to demonstrate a direct interaction between WWOX and SMAD3. Reporter assays, ChIP, confocal microscopy and in silico analyses were employed to determine the effect of WWOX silencing on TGFβ-signaling. Results: WWOX silencing affected cell proliferation, motility, attachment and deregulated expression of genes involved in cell cycle, motility and DNA damage. Interestingly, we detected an enrichment of targets activated by the SMAD3 transcription factor, including significant upregulation of ANGPTL4, FST, PTHLH and SERPINE1 transcripts. Importantly, we demonstrate that the WWOX protein physically interacts with SMAD3 via WW domain 1. Furthermore, WWOX expression dramatically decreases SMAD3 occupancy at the ANGPTL4 and SERPINE1 promoters and significantly quenches activation of a TGFβ responsive reporter. Additionally, WWOX expression leads to redistribution of SMAD3 from the nuclear to the cytoplasmic compartment. Since the TGFβ target ANGPTL4 plays a key role in lung metastasis development, we performed a meta-analysis of ANGPTL4 expression relative to WWOX in microarray datasets from breast carcinomas. We observed a significant inverse correlation between WWOX and ANGPTL4. Furthermore, the WWOXlo/ANGPTL4hi cluster of breast tumors is enriched in triple-negative and basal-like sub-types. Tumors with this gene expression signature could represent candidates for anti-TGFβ targeted therapies. Conclusions: We show for the first time that WWOX modulates SMAD3 signaling in breast cells via direct WW-domain mediated binding and potential cytoplasmic sequestration of SMAD3 protein. Since loss of WWOX expression increases with breast cancer progression and it behaves as an inhibitor of SMAD3 transcriptional activity these observations may help explain, at least in part, the paradoxical pro-tumorigenic effects of TGFβ signaling in advanced breast cancer.Fil: Ferguson, Brent W.. University of Texas; Estados UnidosFil: Gao, Xinsheng. University of Texas; Estados UnidosFil: Zelazowski, Maciej J.. University of Texas; Estados UnidosFil: Lee, Jaeho. University of Texas; Estados UnidosFil: Jeter, Collene R.. University of Texas; Estados UnidosFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aldaz, Claudio Marcelo. University of Texas; Estados Unido

Comparação entre espécies arbóreas nativas e exóticas (Arboreto linear) em Foz do Iguaçu, PR.

bitstream/item/101390/1/PA-1999-Carvalho-ComparacaoEspecies.pd

Plantio de comprovação de arariba-rosa (Centrolobium robustum (Vellozo) Martius ex Bentham.

bitstream/item/101402/1/PA-1999-Carvalho-PlantioComprovacao.pd

Plantio comprobatório de sobrasil (Colubrina glandulosa Perkins var. Reitzii (M.C. Johnston) M.C. Johnston).

bitstream/item/101397/1/PA-1999-Carvalho-PlantioComprobatorio.pd

Comparação entre espécies nativas com one-tree-plot em Foz do Iguaçu, PR (II).

bitstream/item/101386/1/PA-1999-Carvalho-ComparacaoEspecies.pd