Enzymatic Glucose Based Bio batteries: Bioenergy to Fuel Next Generation Devices

[EN] This article consists of a review of the main concepts and paradigms established in the field of biological fuel cells or biofuel cells. The aim is to provide an overview of the current panorama, basic concepts, and methodologies used in the field of enzymatic biofuel cells, as well as the applications of these bio-systems in flexible electronics and implantable or portable devices. Finally, the challenges needing to be addressed in the development of biofuel cells capable of supplying power to small size devices with applications in areas related to health and well-being or next-generation portable devices are analyzed. The aim of this study is to contribute to biofuel cell technology development; this is a multidisciplinary topic about which review articles related to different scientific areas, from Materials Science to technology applications, can be found. With this article, the authors intend to reach a wide readership in order to spread biofuel cell technology for different scientific profiles and boost new contributions and developments to overcome future challenges.Financial support from the Spanish Ministry of Science, Innovation and University, through the State Program for Talent and Employability Promotion 2013-2016 by means of Torres Quevedo research contract in the framework of Bio2 project (PTQ-14-07145) and from the Instituto Valenciano de Competitividad Empresarial-IVACE-GVA (BioSensCell project)Buaki-Sogo, M.; García-Carmona, L.; Gil Agustí, MT.; Zubizarreta Saenz De Zaitegui, L.; García Pellicer, M.; Quijano-Lopez, A. (2020). Enzymatic Glucose Based Bio batteries: Bioenergy to Fuel Next Generation Devices. Topics in Current Chemistry (Online). 378(6):1-28. https://doi.org/10.1007/s41061-020-00312-8S1283786Schlögl R (2015) The revolution continues: Energiewende 2.0. 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The Role of Actin Turnover in Retrograde Actin Network Flow in Neuronal Growth Cones

The balance of actin filament polymerization and depolymerization maintains a steady state network treadmill in neuronal growth cones essential for motility and guidance. Here we have investigated the connection between depolymerization and treadmilling dynamics. We show that polymerization-competent barbed ends are concentrated at the leading edge and depolymerization is distributed throughout the peripheral domain. We found a high-to-low G-actin gradient between peripheral and central domains. Inhibiting turnover with jasplakinolide collapsed this gradient and lowered leading edge barbed end density. Ultrastructural analysis showed dramatic reduction of leading edge actin filament density and filament accumulation in central regions. Live cell imaging revealed that the leading edge retracted even as retrograde actin flow rate decreased exponentially. Inhibition of myosin II activity before jasplakinolide treatment lowered baseline retrograde flow rates and prevented leading edge retraction. Myosin II activity preferentially affected filopodial bundle disassembly distinct from the global effects of jasplakinolide on network turnover. We propose that growth cone retraction following turnover inhibition resulted from the persistence of myosin II contractility even as leading edge assembly rates decreased. The buildup of actin filaments in central regions combined with monomer depletion and reduced polymerization from barbed ends suggests a mechanism for the observed exponential decay in actin retrograde flow. Our results show that growth cone motility is critically dependent on continuous disassembly of the peripheral actin network

LIM kinases are required for invasive path generation by tumor and tumor-associated stromal cells

Leading cells require LIMK for matrix degradation and invadopodia formation during collective cell migration

Optimization of laccase adsorption-desorption behaviors on multi-walled carbon nanotubes for enzymatic biocathodes

Laccase adsorption-desorption behaviors on the surface of multi-walled carbon nanotubes (MWCNTs) were investigated using spectrophotometry and voltammetry. The optimum condition for laccase adsorption is 5.0 mg/mL of laccase in 0.01 M phosphate-buffered saline (PBS) at pH 5.0. Laccase adsorption is a reversible phenomenon that is dependent upon the nature of MWCNTs and the concentration of ionic strength in the laccase solution. Chitosan was functionalized as a nanoporous reservoir to minimize laccase desorption. Chitosan was found to protect approximately 97.2% of the adsorbed laccase from MWCNTs during the first six hours of observation. The three-dimensional (3D) biocathode, MWCNTs-laccase-chitosan with a 0.2 cm2 geometric area, was shown to have a stable open circuit potential (OCP) of 0.55 V, a current density of 0.33 mA cm-2 at 0.2 V vs. saturated calomel electrode (SCE), and a stable current for 20 hours of successive measurements. This report provides a new insight into the study of a high-performance laccase-based biocathode via optimization of adsorption and minimization of desorption phenomena