11 research outputs found

    Collider Scenario Implications of ASST Operation Superconducting Super Collider Laboratory Collider Scenario Implications of ASST Operation * Collider Scenario Implications of ASST Operation

    No full text
    Abstract After the achievement of the SSCL's Congressionally mandated milestone, the Accelerator System String Test (ASST) half cell under went a series of power tests. These tests involved quenches induced in various configurations and in power levels up to the maximum operation point. These tests have produced data which has defined various parameters and requirements for the individual elements and system

    Enhancing Multistep DNA Processing by Solid-Phase Enzyme Catalysis on Polyethylene Glycol Coated Beads

    No full text
    Covalent immobilization of enzymes on solid supports provides an alternative approach to homogeneous biocatalysis by adding the benefits of simple enzyme removal, improved stability, and adaptability to automation and high-throughput applications. Nevertheless, immobilized (IM) enzymes generally suffer from reduced activity compared to their soluble counterparts. The nature and hydrophobicity of the supporting material surface can introduce enzyme conformational change, spatial confinement, and limited substrate accessibility, all of which will result in loss of the immobilized enzyme activity. In this work, we demonstrate through kinetic studies that flexible polyethylene glycol (PEG) moieties modifying the surface of magnetic beads improve the activity of covalently immobilized DNA replication enzymes. PEG-modified immobilized enzymes were utilized in library construction for Illumina next-generation sequencing (NGS) increasing the read coverage across AT-rich regions
    corecore