Haemophilus paraphrophilus brain abscess in a 9-year-old boy: Case report and literature review

Human diseases due to A. paraphrophilus aren’t usual. The following case report describes the first isolated case of A. paraphrophilus brain abscess in our laboratory. A 9-year-old boy presented to pediatric emergencies for frontal headache, vomiting, blurred vision and left hemiparesis. Radiological diagnosis consists with a frontal abscess. Gram staining of purulent samples showed abundant neutrophils with gram negative bacilli. Culture was made on blood agar, chocolate agar, Chapman’s agar and incubated in 5% CO2 at 37°C for 24 hours. Positive culture was detected only on chocolate agar. It was monomicrobial with small yellowish non-hemolytic colonies. Gram stain of colonies showed pleomorphic gram-negative coccobacilli. The strain required V factor for growth. The isolated strain was sensitive to all antibiotics tested.  The interest of this case is that it shows the emergence of A. paraphrophilus as a causative agent of brain abscesses on pediatric population without associated congenital heart disease. It may also help identifying risk factors of these infections and how to prevent them

Haemophilus paraphrophilus brain abscess in a 9-year-old boy: Case report and literature review

Human diseases due to A. paraphrophilus aren’t usual. The following case report describes the first isolated case of A. paraphrophilus brain abscess in our laboratory. A 9-year-old boy presented to pediatric emergencies for frontal headache, vomiting, blurred vision and left hemiparesis. Radiological diagnosis consists with a frontal abscess. Gram staining of purulent samples showed abundant neutrophils with gram negative bacilli. Culture was made on blood agar, chocolate agar, Chapman’s agar and incubated in 5% CO2 at 37°C for 24 hours. Positive culture was detected only on chocolate agar. It was monomicrobial with small yellowish non-hemolytic colonies. Gram stain of colonies showed pleomorphic gram-negative coccobacilli. The strain required V factor for growth. The isolated strain was sensitive to all antibiotics tested.  The interest of this case is that it shows the emergence of A. paraphrophilus as a causative agent of brain abscesses on pediatric population without associated congenital heart disease. It may also help identifying risk factors of these infections and how to prevent them

Antibiotic resistance and serotype distribution of nasopharyngeal isolates of Streptococcus pneumoniae from children in Marrakech region (Morocco)

Summary: Monitoring of Streptococcus pneumoniae antibiotic resistance is of great importance due to the frequency of strains becoming increasingly resistant to antibiotics. In this study, we report the antibiotic susceptibility of the serotypes of S. pneumoniae strains isolated from healthy children aged 1–24 months in the Marrakech region of Morocco. Resistance to penicillin (38.7%) was frequently associated with resistance to other antibiotics. The highest rates of resistance were to cotrimoxazole (trimethoprim/sulfamethoxazole) (49.3%), erythromycin (48.7%), tetracycline (37.3%), lincomycin (35.3%), chloramphenicol (32.7%) and ciprofloxacin (24%). Prisitinamycin and vancomycin were effective against all isolated pneumococcal strains (100% sensitive strains). Gentamycin demonstrated good efficacy on S. pneumoniae, with 98.7% of strains being sensitive. Multidrug resistance characterized 43.33% of all studied strains. Of the multidrug-resistant strains, 36.92% were resistant to erythromycin (E), tetracycline (T) and cotrimoxazole (Co: sulfamethoxazole-trimethoprim) (phenotype ETCo, n = 24), and 20% had decreased susceptibility to beta-lactams, erythromycin and cotrimoxazole (phenotype PECo, n = 13). A total of 29.23% of S. pneumoniae strains exhibited combined resistance to four antibiotics (phenotype PETCo, n = 19). This study reports the status of resistance and multiresistance of S. pneumoniae strains in the Marrakech region of Morocco. Keywords: S. pneumoniae, Antibiotic resistance, Serogroup, Multiresistanc