The use of microencapsulated feeds to replace live food organisms in shrimp hatcheries

Abstract only.An adequate supply of hatchery produced shrimp fry is the major constraint to the intensification and growth of shrimp culture practices. If even 20% of the more than 500,000 ha of the world's existing tropical and sub-tropical brackishwater ponds were to stock at the relatively low density of 50,000 fry/ha/year, it would take thousands of new hatcheries to produce the 25 billion fry required. The availability of artificially produced diets to replace cultured live food organisms would alleviate many of the problems currently limiting shrimp hatchery production by: (i) reducing the level of technical skill required to operate a hatchery; (ii) assuring a reliable supply of a nutritionally balanced larval feed; (iii) reducing sources of contamination and larval disease; and (iv) simplifying hatchery design and capital cost requirements, thereby facilitating small scale hatchery development. Aquatic farms has been working with the Mars Microencapsulation Research Group (MMRG) to develop techniques for adapting current shrimp hatchery technology and design so that MMRG feeds can be used in existing hatcheries as a live feed replacement. Feeding trials have been conducted in commercial hatcheries in Hawaii, Malaysia and Thailand. The results of these trials and the techniques employed are discussed. Growth and survival of larvae fed microencapsulated diets as total or partial replacement of live foods was comparable to larvae cultured in control tanks using the standard operating procedures of the hatchery in which the trials were conducted. In trials to date, larval survival from nauplii to postlarvae has been as high as 70%

Macondo-1 well oil-derived polycyclic aromatic hydrocarbons in mesozooplankton from the northern Gulf of Mexico

Copyright 2012 by the American Geophysical UnionMesozooplankton (>200 μm) collected in August and September of 2010 from the northern Gulf of Mexico show evidence of exposure to polycyclic aromatic hydrocarbons (PAHs). Multivariate statistical analysis revealed that distributions of PAHs extracted from mesozooplankton were related to the oil released from the ruptured British Petroleum Macondo-1 (M-1) well associated with the R/VDeepwater Horizon blowout. Mesozooplankton contained 0.03–97.9 ng g−1 of total PAHs and ratios of fluoranthene to fluoranthene + pyrene less than 0.44, indicating a liquid fossil fuel source. The distribution of PAHs isolated from mesozooplankton extracted in this study shows that the 2010 Deepwater Horizon spill may have contributed to contamination in the northern Gulf of Mexico ecosystem

An improved strategy for building brackishwater culture ponds with iron pyrite soils in mangrove swamps

Abstract only.The problems associated with acid sulfate soil limit the potential utilization of vast coastal areas of mangrove swamps for brackishwater aquaculture. There is an estimated 4.8 million ha of mangrove area in the ASEAN countries alone. Until recently, most attempts to build earthen ponds in these areas have yielded poor results. Aquatic Farms, as technical consultants for a 250 ha-prawn farm in Johore Peninsula, Malaysia, developed a construction technique that utilized the volcano-like burrow mounds of the mud lobster (Thalassina anomala) to cover and seal pond embankments that has minimized the culture problems usually experienced with iron pyrite soil. The strategy, pond design and construction technique are described. Pond dynamics and performance are discussed since the commencement of culture operations and these are compared with a nearby prawn farm that was constructed using conventional techniques. A cost benefit analysis is given in conclusion

UV-induced Hyperphosphorylation of Replication Protein A Depends on DNA Replication and Expression of ATM Protein

Exposure to DNA-damaging agents triggers signal transduction pathways that are thought to play a role in maintenance of genomic stability. A key protein in the cellular processes of nucleotide excision repair, DNA recombination, and DNA double-strand break repair is the single-stranded DNA binding protein, RPA. We showed previously that the p34 subunit of RPA becomes hyperphosphorylated as a delayed response (4–8 h) to UV radiation (10–30 J/m(2)). Here we show that UV-induced RPA-p34 hyperphosphorylation depends on expression of ATM, the product of the gene mutated in the human genetic disorder ataxia telangiectasia (A-T). UV-induced RPA-p34 hyperphosphorylation was not observed in A-T cells, but this response was restored by ATM expression. Furthermore, purified ATM kinase phosphorylates the p34 subunit of RPA complex in vitro at many of the same sites that are phosphorylated in vivo after UV radiation. Induction of this DNA damage response was also dependent on DNA replication; inhibition of DNA replication by aphidicolin prevented induction of RPA-p34 hyperphosphorylation by UV radiation. We postulate that this pathway is triggered by the accumulation of aberrant DNA replication intermediates, resulting from DNA replication fork blockage by UV photoproducts. Further, we suggest that RPA-p34 is hyperphosphorylated as a participant in the recombinational postreplication repair of these replication products. Successful resolution of these replication intermediates reduces the accumulation of chromosomal aberrations that would otherwise occur as a consequence of UV radiation