2 research outputs found
AJICAP-M: Traceless Affinity Peptide Mediated Conjugation Technology for Site-Selective Antibody–Drug Conjugate Synthesis
A traceless
site-selective conjugation method, “AJICAP-M”,
was developed for native antibodies at sites using Fc-affinity peptides,
focusing on Lys248 or Lys288. It produces antibody–drug conjugates
(ADCs) with consistent drug-to-antibody ratios, enhanced stability,
and simplified manufacturing. Comparative in vivo assessment demonstrated
AJICAP-M’s superior stability over traditional ADCs. This technology
has been successfully applied to continuous-flow manufacturing, marking
the first achievement in site-selective ADC production. This manuscript
outlines AJICAP-M’s methodology and its effectiveness in ADC
production
Feasibility Study on Continuous Flow Controlled/Living Anionic Polymerization Processes
A practical microchemical
reaction system for keeping process flow
at defined conditions, which is one of the key issues of industrial
production, was developed. Controlled/living anionic polymerization
was chosen as a test reaction because the molecular weight and molecular
weight distribution of polymer products are quite sensitive to the
relative flow rate of an initiator solution and that of a monomer
solution. The polymerization of styrene in THF/hexane was carried
out using a flow microreactor system consisting of two T-shaped micromixers
and two microtube reactors using Smoothflow pumps at 0 °C. PolyÂ(styrene)
with higher molecular weight such as Mn > 10000 could be synthesized
using <i>s</i>-BuLi (Mn = 14 000, <i>M</i><sub>w</sub>/<i>M</i><sub>n</sub> = 1.11). <i>n</i>-BuLi could also be used as an initiator. The continuous operation
was performed for 3 h without any problems to obtain ca. 1 kg of the
polymer, indicating the feasibility of continuous flow processes for
controlled/living anionic polymerization on a relatively large scale