2 research outputs found
Synthesis of Calcium Bisphosphonate/Calcium Polyacrylate Spheres for Gene Delivery
Calcium
bisphosphonate/calcium polyacrylate spheres were synthesized
by a facile method and applied for the first time as gene vectors
for transfection. The colloidal spheres of the PAA–Ca<sup>2+</sup>–H<sub>2</sub>O complex, formed by sodium polyacrylate and
calcium ions in the solution, were used as template to synthesize
a spherical PAA–Ca<sup>2+</sup>–BPMP composite (CaBPMP/CaPAA)
in the presence of 1,4-bisÂ(phosphomethyl)Âpiperazine (BPMP). The CaBPMP/CaPAA
composite exhibits uniform and well-dispersed spheres with a particle
size of about 200 nm as expected. The cytotoxicity assays confirm
that CaBPMP/CaPAA spheres are quite safe for different cells even
at a high concentration of 500 μg/mL. In vitro transfection
results show that CaBPMP/CaPAA spheres serving as gene vectors are
capable of transferring exogenous genes into different cells with
about 25% of transfection efficiency and good reproducibility. The
transfection capacity of CaBPMP/CaPAA spheres may be attributed to
the controllable sphere morphology, low cytotoxicity, moderate DNA
loading capacity, and bioresorbable property. The application of calcium
phosphonates with adjustable surface properties derived from the different
organic groups of phosphonic acid in gene delivery provides a new
design idea for gene vectors
Construction of a ferroptosis and hypoxia-related gene signature in cervical cancer to assess tumour immune microenvironment and predict prognosis
This study aimed to investigate the potential role of ferroptosis/hypoxia-related genes in cervical cancer to improve early management and treatment of cervical cancer. All data were downloaded from public databases. Ferroptosis/hypoxia-related genes associated with cervical cancer prognosis were selected to construct a risk score model. The relationship between risk score and clinical features, immune microenvironment and prognosis were analysed. Risk score model was constructed based on eight signature genes. Drug prediction analysis showed that bevacizumab and cisplatin were related to vascular endothelial growth factor A. Risk score, as an independent prognostic factor of cervical cancer, had a good survival prediction effect. The two groups differed significantly in degree of immune cell infiltration, gene expression, tumour mutation burden and somatic variation. We developed a novel prognostic gene signature combining ferroptosis/hypoxia-related genes, which provides new ideas for individual treatment of cervical cancer. Ferroptosis, hypoxia and immune regulation play important roles in cervical cancer progression. In this study, we developed a novel prognostic signature combining ferroptosis and hypoxia-related genes, which provides new ideas for individual treatment of cervical cancer patients. The risk score established by ferroptosis and hypoxia-related gene as an independent prognostic factor of cervical cancer has a good survival prediction effect. High and low risk groups showed significant differences in TIME, prognosis, biological metabolic pathway and tumour mutation burden. In addition, we found drugs associated with signature genes. In short, this study has laid a theoretical foundation for exploring the related molecular mechanisms and prognosis of cervical cancer. It also contributes to the exploration of clinical management and treatment.</p